Sturgeon (family Acipenseridae) are regarded as living fossils due to their ancient origin and exceptionally slow evolution. To extend our knowledge of fish cardiac excitability to a Chondrostei fish, we examined electrophysiological phenotype of the Siberian sturgeon ( Acipenser baerii) heart with recordings of epicardial ECG, intracellular action potentials (APs), and sarcolemmal ion currents. Epicardial ECG of A. baerii had the typical waveform of the vertebrate ECG with Q-T interval (average duration of ventricular AP) of 650 ± 30 ms and an intrinsic heart rate of 45.5 ± 5 beats min−1 at 20°C. Similar to other fish species, atrial AP was shorter in duration (402 ± 33 ms) than ventricular AP (585 ± 40) ( P < 0.05) at 20°C. Densities of atrial and ventricular Na+ currents were similar (−47.6 ± 4.5 and −53.2 ± 5.1 pA/pF, respectively) and close to the typical values of teleost hearts. Two major K+ currents, the inward rectifier K+ current ( IK1), and the delayed rectifier K+ current ( IKr) were found under basal conditions in sturgeon cardiomyocytes. The atrial IKr (3.3 ± 0.2 pA/pF) was about twice as large as the ventricular IKr (1.3 ± 0.4 pA/pF) ( P < 0.05) conforming to the typical pattern of teleost cardiac IKr. Divergent from other fishes, the ventricular IK1 was remarkably small (−2.5 ± 0.07 pA/pF) and not different from that of the atrial myocytes (−1.9 ± 0.06 pA/pF) ( P > 0.05). Two ligand-gated K+ currents were also found: ACh-activated inward rectifier ( IKACh) was present only in atrial cells, while ATP-sensitive K+ current ( IKATP) was activated by a mitochondrial blocker, CCCP, in both atrial and ventricular cells. The most striking difference to other fishes appeared in Ca2+ currents ( ICa). In atrial myocytes, ICa was predominated by nickel-sensitive and nifedipine-resistant T-type ICa, while ventricular myocytes had mainly nifedipine-sensitive and nickel-resistant L-type ICa. ICaT/ ICaL ratio of the sturgeon atrial myocytes (2.42) is the highest value ever measured for a vertebrate species. In ventricular myocytes, ICaT/ ICaL ratio was 0.09. With the exception of the large atrial ICaT and small ventricular IK1, electrical excitability of A. baerii heart is similar to that of teleost hearts.