Spectrophotometric Quantification of Iron in Different Pharmaceuticals

Quantification of iron in different pharmaceuticals can be performed by different analytical methods. In this study, a rapid, sensitive and simple spectrophotometric method was used for the determination of iron (II) in different iron tablets. A spectrophotometric method is based on the reaction of iron (II) with 1,10-phenanthroline to form an orange-red chelating complex in an acidic medium. The maximum absorbance of the chelating complex was measured at 510 nm. The Beer Lambert’s law was found to be obeyed in the range of 0.4-4.0 mg/L of iron (II). Out of ten samples analyzed, the observed amount of iron (II) in nine samples were range from 105 to 96 mg whereas in one sample it was observed only 81 mg per 100 mg of the labeled amount of iron(II) by the manufacturer. Thus the results obtained by the spectrophotometric method using 1,10-phenanthroline as a color developing agent were nearly equal to the claimed values of iron (II) in different iron tablets from manufacturing companies.

Exploring the Complexation of Counterion in Novel Family of Polyelectrolytes with Unexpected Solubility Behaviour

In the present paper we study the effect of complexation in linear negatively charged polyelectrolytes with different alkali ions. With combination of IR-spectroscopy, X-ray diffraction and nanocalorimetry, we attempted to explain unusual solubility, crystallinity and thermal stability of these polymers. The increase of thermal stability and insolubility in water in series of semi-crystalline polysalts as K+ ≤ H+ <Na+ was explained by effectiveness of formation of chelating complex. Insoluble in water sodium salt shows the highest thermal stability of crystal phase up to . In contrast, well soluble in water amorphous lithium salt does not self-organize in chelating complex and is presented in ionic form.

Siderophore Cephalosporin Cefiderocol Utilizes Ferric Iron Transporter Systems for Antibacterial Activity againstPseudomonas aeruginosa

Cefiderocol (S-649266) is a novel parenteral siderophore cephalosporin conjugated with a catechol moiety at the 3rd-position side chain. Thein vitroactivity of cefiderocol againstPseudomonas aeruginosawas enhanced in the iron-depleted condition where that of ceftazidime was not affected. The monitoring of [Thiazole-14C]-cefiderocol revealed the increased intracellular accumulation of cefiderocol in theP. aeruginosacells incubated under iron-depleted condition compared with those incubated under iron-sufficient condition. Cefiderocol was shown to have potent chelating activity with ferric iron, and extracellular iron was efficiently transported intoP. aeruginosacells in the presence of cefiderocol as well as siderophores, while enhanced transport of extracellular ferric iron was not observed when one of the hydroxyl groups of catechol moiety of cefiderocol was substituted with a methoxy group. We conclude that cefiderocol forms a chelating complex with iron, which is actively transported intoP. aeruginosacells via iron transporters, resulting in potent antibacterial activity of cefiderocol againstP. aeruginosa.

Analysis of SNP markers for chicken blue-shelled gene using PCR-SSCP

The oocyan (O) gene determines blue shell pigmentation of domesticated chicken. The O locus is closely linked to ALEV1 on chromosome 1 with a genetic distance of 2.3 cM. Blue eggshells contain a protoporphyrin, biliverdin and zinc biliverdin chelating complex, which belongs to the porphyrin metabolic pathway. A bio-informatic approach was first used to BLAST for the genes of the enzymes in the porphyrin pathway; no gene was detected to specify the O locus. Due to a limited number of simple sequence repeat (SSR) markers around the O locus, polymerase chain reaction–single-strand conformational polymorphism (PCR-SSCP) was then employed to search for candidate single nucleotide polymorphism (SNP) markers. The sequence bearing a marker was found to consist of two transversions at Chr1:61754089T→A and Chr1:61754175A→T, respectively. Population screening showed that 81% of homozygous blue-shelled layers (OO) were classified as AA genotype at the SNP loci, while 89% of heterozygous blue-shelled layers (Oo) were AB genotype and 93% of tinted layers (oo) were BB genotype. The results indicated that there is a close association between O locus and SNP locus, suggesting that the marker locus could be used as a molecular marker in breeding for blue-shelled chickens.