ACE Inhibitory, Antitumor and Antioxidant Activities of Submerged Culture Materials of Three Medicinal Mushrooms

Mushrooms have been highly valued as tonic foods and herb remedies since ancient times, and have been increasingly concerned because of their biological and pharmacological activities recognized through recent scientific research. Antitumor, antioxidant and ACE inhibitory activities in vitro of liquid fermentation materials from Ramaria botrytoides, Lyophyllum decastes and Mycoleptodonoides aitchisonii were investigated in this study. Water extracts from fermented mycelia and supernatant materials were used to measure their antitumor effect by MTT assay, antioxidant capacity by kit, and ACE inhibitory activity by RP-HPLC. Results showed that mycelia extracts from Ramaria botrytoides has significantly higher anti-proliferative effect on human HCC cell line SMMC7721 (IC50=0.284±0.005mg/ml, p<0.001). Total antioxidant activity of mycelia extracts of Ramaria botrytoides (0.711±0.053U/mg), inhibitory effect of mycelia extracts of Mycoleptodonoidesaitchisonii on hydroxyl radical (86.153±1.763U/mg) and superoxide anion radical (7.363±0.280 U/g) were higher than others significantly (p<0.001). ACE inhibitory activity of mycelia extracts of Lyophyllum decastes (IC50=1.637±0.057mg/ml) was significantly higher than others (p<0.001). Results demonstrated that liquid fermented mycelium of Ramaria botrytoides was potent source of natural compound with antitumor and antioxidant potential. Mycelium of Lyophyllum decastes was potent source of natural compound with ACE inhibitory activity.