Endophytic bacteria promote the quality of Lyophyllum decastes by improving non-volatile taste components of mycelia

2021 ◽  
Vol 336 ◽  
pp. 127672
Author(s):  
Man Xu ◽  
Hong-Yan Bai ◽  
Wan-Qiu Fu ◽  
Kai Sun ◽  
Hong-Wei Wang ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Lyophyllum Decastes

scholarly journals Endophytic bacteria and plant immunity

2020 ◽  
Author(s):  
I. V. Maksimov ◽  
M. Yu. Shein ◽  
R. M. Khairullin
Keyword(s):  
Development Strategy ◽  
Host Defense ◽  
Endophytic Bacteria ◽  
Plant Protection ◽  
Plant Immunity ◽  
Plant Protection Products ◽  
Agricultural Products ◽  
The Development Strategy

Diseases annually cause significant crop losses and reduced quality of agricultural products. The development strategy of new environmentally friendly plant protection products should consider the role of the microbiome in host defense.

Endophytic bacteria enhanced growth, fruit yield and quality in Phalsa (Grewia asiatica L.)

2020 ◽  
pp. 41-46
Author(s):  
Hassan Ahmad ◽  
Muhammad Jafar Jaskani ◽  
Waqar Shafqat ◽  
Muhammad Naveed ◽  
Summar Abbas Naqvi ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Block Design ◽  
Insect Pest ◽  
Combined Treatment ◽  
Growth Yield ◽  
Reducing Sugar ◽  
Fruit Yield ◽  
Growth And Yield ◽  
Yield And Quality

There is an immense need to replace chemical fertilizers with biofertilizers to address the soil, environment, and health issues. Endophytic bacteria act as biocontrol agents and promote plant growth and yield. Present study was designed to evaluate the microbial effect of endophytic bacteria on growth, fruit yield and quality of phalsa (Grewia asiatica L.). Three years old healthy, disease and insect-pest free plants were selected for the study. Experiment was laid out in randomized complete block design with four treatments; control, two bacteria such as Burkholderia phytofirmans strain PsJN, Bacillus sp. strain MN54 and their combination (PsJN + MN54) with three replications. The treatments were applied after pruning (January) and flowering (March) as plants need nutrition for both vegetative and reproductive growth. Combined application of PsJN + MN54 resulted in greater vegetative and reproductive growths of plants i.e. number of shoots/plant (29.6), number of leaves/shoot (15.9), number of fruit clusters/shoot (14.0), number of fruits/cluster (14.1), fruit weight (13.7 g) and yield/plant (8.8 kg) than PsJN or MN54 alone and control. Fruit biochemical characteristic i.e. TSS (8.78 °Brix), TA (0.53%), ascorbic acid (44.44 mg 100 mL-1), total sugar (12.29%), reducing sugar (9.59%) and non-reducing sugar (4.92%) contents were also higher in plants treated with PsJN + MN54. All growth, yield and biochemical parameters correlated positively with each other except titratable acidity. Based on performance, the combined treatment (PsJN + MN54) can be applied at two stages, after pruning and at flowering, for better growth, yield and quality of phalsa crop.

scholarly journals ENDOPHYTIC BACTERIA USED AS BIOINOCULANTS IN MICROPROPAGATED BANANA SEEDLINGS

2017 ◽  
Vol 39 (2) ◽  
Author(s):  
GLEIKA LARISSE OLIVEIRA DORASIO DE SOUZA ◽  
DEIVISSON FERREIRA DA SILVA ◽  
SILVIA NIETSCHE ◽  
ADELICA APARECIDA XAVIER ◽  
MARLON CRISTIAN TOLEDO PEREIRA
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Microbial Inoculants ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

ABSTRACT The use of bio-fertilizers and microbial inoculants that promote plant growth and increased yield has been accepted as an alternative to reduce the use of chemical fertilizers. The objective of the present study was to evaluate the effect of plant growth promoting bacteria inoculation on growth and quality of micropropagated banana seedlings ‘Prata Anã’. The experimental design was completely randomized, with four replications and the treatments consisted of 24 isolates of endophytic bacteria. The isolates EB-50 (Bacillus sp.) and EB-133 (Bacillus amyloliquefaciens) characterized as diazotrophic, the EB-51 (Bacillus pumilus) indicate for inorganic phosphate solubilization and EB-55(Bacillus subtilis) and EB-40 (Bacillus sp.) indole-3-acetic acid producers have provided significant increases for length, pseudostem diameter, fresh masses and dry masses in ‘Prata Anã’ micropropagated banana seedlings.

scholarly journals Quality of Immature and Mature Pepper (Capsicum annuum L.) Seeds in Relation to Bio-Priming with Endophytic Pseudomonas and Bacillus spp.

Horticulturae ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 75
Author(s):  
Kutay Coskun Yildirim ◽  
Didem Canik Orel ◽  
Hilal Okyay ◽  
Mukerrem Melis Gursan ◽  
Ibrahim Demir
Keyword(s):  
Endophytic Bacteria ◽  
Seedling Emergence ◽  
Control Group ◽  
Subtilis Strain ◽  
Bacillus Mojavensis ◽  
Immature Seeds ◽  
Bacillus Spp ◽  
Mock Treatment ◽  
Mature Seeds

Fruit maturity for seed production can occur at various times because of the continual flowering of pepper plants. Accordingly, seeds with different maturity are acquired as the fruits are collected in a single harvest. Immature seeds obtained in this harvest may lead to a decrease in the quality of seed lots. Therefore, this research aimed to evaluate the influence of four different endophytic bacteria strains (Pseudomonas fluorescens strain L5b, Pseudomonas gessardii strain L13, Bacillus subtilis strain Bs1 and Bacillus mojavensis strain ApBm) on germination and seedling vigor of immature and mature bell pepper seeds. To obtain seeds with different maturity levels, fruits were collected 45–49 days after flowering for immature seeds and 65–69 days for mature seeds. The effectiveness of these bacteria strains was examined by coating seeds with four different endophytic bacteria strains separately. Additionally, to see the activity of endophytic bacteria more clearly, a mock treatment with sterile water was added to the experiment as a control (+) group. Bio-priming (especially strain Bs1 and L13) improved germination and seedling emergence characteristics of both immature and mature seed lots compared to control groups (p < 0.05). The results demonstrate that bio-priming with beneficial endophytic bacteria can be used to stimulate the quality of both immature and mature seeds from the pepper.

Immunoferritin localization of intracellular antigens in positively stained frozen sections

1978 ◽  
Vol 36 (2) ◽  
pp. 168-169
Author(s):  
K. T. Tokuyasu
Keyword(s):  
Surface Tension ◽  
Water Surface ◽  
Thin Layers ◽  
The Other ◽  
Positive Staining ◽  
Frozen Sections ◽  
The Past ◽  
Ultrathin Frozen Sections ◽  
Definition Of

During the past investigations of immunoferritin localization of intracellular antigens in ultrathin frozen sections, we found that the degree of negative staining required to delineate u1trastructural details was often too dense for the recognition of ferritin particles. The quality of positive staining of ultrathin frozen sections, on the other hand, has generally been far inferior to that attainable in conventional plastic embedded sections, particularly in the definition of membranes. As we discussed before, a main cause of this difficulty seemed to be the vulnerability of frozen sections to the damaging effects of air-water surface tension at the time of drying of the sections.Indeed, we found that the quality of positive staining is greatly improved when positively stained frozen sections are protected against the effects of surface tension by embedding them in thin layers of mechanically stable materials at the time of drying (unpublished).

Lithography below 100 nm

1983 ◽  
Vol 41 ◽  
pp. 96-99
Author(s):  
L. D. Jackel
Keyword(s):  
Spatial Distribution ◽  
Electron Beam ◽  
Electron Scattering ◽  
Electron Beam Lithography ◽  
Substrate Material ◽  
Local Electron ◽  
Electron Dose ◽  
Positive Resist ◽  
Exposure Process

Most production electron beam lithography systems can pattern minimum features a few tenths of a micron across. Linewidth in these systems is usually limited by the quality of the exposing beam and by electron scattering in the resist and substrate. By using a smaller spot along with exposure techniques that minimize scattering and its effects, laboratory e-beam lithography systems can now make features hundredths of a micron wide on standard substrate material. This talk will outline sane of these high- resolution e-beam lithography techniques.We first consider parameters of the exposure process that limit resolution in organic resists. For concreteness suppose that we have a “positive” resist in which exposing electrons break bonds in the resist molecules thus increasing the exposed resist's solubility in a developer. Ihe attainable resolution is obviously limited by the overall width of the exposing beam, but the spatial distribution of the beam intensity, the beam “profile” , also contributes to the resolution. Depending on the local electron dose, more or less resist bonds are broken resulting in slower or faster dissolution in the developer.

Convergent Beam Electron Diffraction

1978 ◽  
Vol 36 (3) ◽  
pp. 304-315
Author(s):  
G. Lehmpfuhl
Keyword(s):  
Electron Diffraction ◽  
Diffraction Pattern ◽  
Crystal Surface ◽  
Diffraction Spot ◽  
Crystal Thickness ◽  
Large Area ◽  
Electron Microscopic ◽  
Additional Information ◽  
Microscopic Investigations

Introduction In electron microscopic investigations of crystalline specimens the direct observation of the electron diffraction pattern gives additional information about the specimen. The quality of this information depends on the quality of the crystals or the crystal area contributing to the diffraction pattern. By selected area diffraction in a conventional electron microscope, specimen areas as small as 1 µ in diameter can be investigated. It is well known that crystal areas of that size which must be thin enough (in the order of 1000 Å) for electron microscopic investigations are normally somewhat distorted by bending, or they are not homogeneous. Furthermore, the crystal surface is not well defined over such a large area. These are facts which cause reduction of information in the diffraction pattern. The intensity of a diffraction spot, for example, depends on the crystal thickness. If the thickness is not uniform over the investigated area, one observes an averaged intensity, so that the intensity distribution in the diffraction pattern cannot be used for an analysis unless additional information is available.

Observability of Very Thick Specimen by Using Transmission Scanning Electron Microscope

1971 ◽  
Vol 29 ◽  
pp. 26-27
Author(s):  
K. Shibatomi ◽  
T. Yamanoto ◽  
H. Koike
Keyword(s):  
Electron Microscope ◽  
Image Quality ◽  
Scanning Electron Microscope ◽  
Chromatic Aberration ◽  
Image Contrast ◽  
Objective Lens ◽  
Thick Specimen ◽  
Transmission Electron ◽  
Scanning Electron

In the observation of a thick specimen by means of a transmission electron microscope, the intensity of electrons passing through the objective lens aperture is greatly reduced. So that the image is almost invisible. In addition to this fact, it have been reported that a chromatic aberration causes the deterioration of the image contrast rather than that of the resolution. The scanning electron microscope is, however, capable of electrically amplifying the signal of the decreasing intensity, and also free from a chromatic aberration so that the deterioration of the image contrast due to the aberration can be prevented. The electrical improvement of the image quality can be carried out by using the fascionating features of the SEM, that is, the amplification of a weak in-put signal forming the image and the descriminating action of the heigh level signal of the background. This paper reports some of the experimental results about the thickness dependence of the observability and quality of the image in the case of the transmission SEM.

The Current Situation in Chemical Stabilization of Biological Materials

1972 ◽  
Vol 30 ◽  
pp. 132-133
Author(s):  
John H. Luft
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Molecular Level ◽  
Cross Linking ◽  
Chemical Stabilization ◽  
Specimen Preparation ◽  
Sufficient Condition ◽  
Radio Telescopes

With information processing devices such as radio telescopes, microscopes or hi-fi systems, the quality of the output often is limited by distortion or noise introduced at the input stage of the device. This analogy can be extended usefully to specimen preparation for the electron microscope; fixation, which initiates the processing sequence, is the single most important step and, unfortunately, is the least well understood. Although there is an abundance of fixation mixtures recommended in the light microscopy literature, osmium tetroxide and glutaraldehyde are favored for electron microscopy. These fixatives react vigorously with proteins at the molecular level. There is clear evidence for the cross-linking of proteins both by osmium tetroxide and glutaraldehyde and cross-linking may be a necessary if not sufficient condition to define fixatives as a class.

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