Bovine Coronavirus and the Associated Diseases

Coronaviruses (CoVs) possess the largest and most complex RNA genome (up to 32 kb) that encodes for 16 non-structural proteins regulating RNA synthesis and modification. Coronaviruses are known to infect a wide range of mammalian and avian species causing remarkably diverse disease syndromes. Variable tissue tropism and the ability to easily cross interspecies barriers are the well-known characteristics of certain CoVs. The 21st century epidemics of severe acute respiratory CoV (SARS-CoV), Middle East respiratory CoV and the ongoing SARS-CoV-2 pandemic further highlight these characteristics and emphasize the relevance of CoVs to the global public health. Bovine CoVs (BCoVs) are betacoronaviruses associated with neonatal calf diarrhea, and with winter dysentery and shipping fever in older cattle. Of interest, no distinct genetic or antigenic markers have been identified in BCoVs associated with these distinct clinical syndromes. In contrast, like other CoVs, BCoVs exist as quasispecies. Besides cattle, BCoVs and bovine-like CoVs were identified in various domestic and wild ruminant species (water buffalo, sheep, goat, dromedary camel, llama, alpaca, deer, wild cattle, antelopes, giraffes, and wild goats), dogs and humans. Surprisingly, bovine-like CoVs also cannot be reliably distinguished from BCoVs using comparative genomics. Additionally, there are historical examples of zoonotic transmission of BCoVs. This article will discuss BCoV pathogenesis, epidemiology, interspecies transmission, immune responses, vaccines, and diagnostics.

Bovine-like coronaviruses in domestic and wild ruminants

Coronaviruses (CoVs) produce a wide spectrum of disease syndromes in different mammalian and avian host species. These viruses are well-recognized for their ability to change tissue tropism, to hurdle the interspecies barriers and to adapt ecological variations. It is predicted that the inherent genetic diversity of CoVs caused by accumulation of point mutations and high frequency of homologous recombination is the principal determinant of these competences. Several CoVs (e.g. Severe acute respiratory syndrome-CoV, Middle East respiratory syndrome-CoV) have been recorded to cross the interspecies barrier, inducing different disease conditions in variable animal hosts. Bovine CoV (BCoV) is a primary cause of gastroenteritis and respiratory disease in cattle calves, winter dysentery in lactating cows and shipping fever pneumonia in feedlot cattle. Although it has long been known as a restrictive cattle pathogen, CoVs that are closely related to BCoV have been recognized in dogs, humans and in other ruminant species. Biologic, antigenic and genetic analyses of the so-called ‘bovine-like CoVs’ proposed classification of these viruses as host-range variants rather than distinct virus species. In this review, the different bovine-like CoVs that have been identified in domesticated ruminants (water buffalo, sheep, goat, dromedary camel, llama and alpaca) and wild ruminants (deer, wild cattle, antelopes, giraffes and wild goats) are discussed in terms of epidemiology, transmission and virus characteristics. The presented data denote the importance of these viruses in the persistence of BCoV in nature, spread to new geographical zones, and continuous emergence of disease epidemics in cattle farms.

Other bacterial diseasesPasteurellosis

Pasteurellosis is a zoonosis that occurs worldwide, caused by bacteria of the genus Pasteurella, and other related organisms. Pasteurellosis reported in humans is most frequently caused by the species Pasteurella multocida. In humans, cutaneous infection is most common, but more severe outcomes have been reported, particularly in those with underlying chronic disease. Infection in animals is usually subclinical, but may give rise to a range of clinical symptoms, depending on the host species. Disease in animals usually occurs as a consequence of stress such as overcrowding, chilling, transportation, or as a result of a concurrent infection. In animals, pasteurellosis is known as: shipping fever or pneumonia, transport or transit fever, stockyard pneumonia, bovine pneumonic pasteurellosis, haemorrhagic septicaemia, or avian, bird or fowl cholera. The pasteurella bacterium is commonly present in the mouth and gastrointestinal tract of a wide range of mammals. Transmission to humans occurs after bites, scratches, or licks from infected animals, most frequently from dogs or cats, although infection has been associated with other animals including: cows, pigs, hamsters and rabbits. However, not all patients report a history of direct animal contact. Infection may be prevented through the avoidance of animal bites and the prompt hygienic care of wounds. Health professionals should be aware of the risk of pasterurellosis in immunocompromised patients exposed to companion animals.