Highly Stretchable Bacterial Cellulose Produced by Komagataeibacter hansenii SI1

A new strain of bacteria producing cellulose was isolated from Kombucha and identified as Komagataeibacter hansenii, named SI1. In static conditions, the strain synthesises bacterial nanocellulose with an improved ability to stretch. In this study, utilisation of various carbon and nitrogen sources and the impact of initial pH was assessed in terms of bacterial nanocellulose yield and properties. K. hansenii SI1 produces cellulose efficiently in glycerol medium at pH 5.0–6.0 with a yield of 3.20–3.60 g/L. Glucose medium led to the synthesis of membrane characterised by a strain of 77%, which is a higher value than in the case of another Komagataeibacter species. Supplementation of medium with vitamin C results in an enhanced porosity and improves the ability of bacterial nanocellulose to stretch (up to 123%). The properties of modified membranes were studied by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and mechanical tests. The results show that bacterial nanocellulose produced in SH medium and vitamin C-supplemented medium has unique properties (porosity, tensile strength and strain) without changing the chemical composition of cellulose. The method of production BNC with altered properties was the issue of Polish patent application no. P.431265.

Production of Sphere-Like Bacterial Cellulose In Cultivation Media With Different Carbon Sources: A Promising Sustained Release System of Rifampicin

Bacterial cellulose (BC) production can be performed using a static or stirred culture method. In the static culture method, the BC is obtained presents three-dimensional thinner network structures and excellent mechanical properties. In the agitated culture method, BC is produced in the form of fibrous granules or threads with a lower degree of polymerization, mechanical resistance, and crystallinity than the films formed in static fermentation. Compared with BC membranes, sphere-like BC (SBC) cultured under agitated conditions showed advantages for adsorption due to its larger surface area. The objectives of this work were to obtain SBC, by the bacterial strain Komagataeibacter hansenii ATCC 23769, in agitated cultivation, using media containing different carbon sources carbon sources, such as fructose (FRU), glucose and sucrose (MS1), sucrose (Y) and glucose (Z and HS), aiming to produce supports for sustained release of rifampicin (RIF). SBC has been produced under agitation at 130 rpm and 25°C. SBC obtained were processed to remove bacteria and residues from the culture media and lyophilized. The SBC characterizations were performed by Fourier transform infrared spectroscopy (FTIR), Field emission gun-scanning electron microscopy (FEG-SEM), thermogravimetric analysis (TGA) and by derivative thermogravimetry (DTG). The SBC produced were impregnated with antibiotic RIF and tested for the sustained release capacity of this drug by diffusion method and Frans cell kinetics. SBC that the best results for all tests were produced in FRU, Z and MS1 media, respectively. The results demonstrate the potential of the SBC to contribute to the design of new drug delivery systems with biomedical applications.

Komagataeibacter hansenii CGMCC 3917 alleviates alcohol-induced liver injury by regulating fatty acid metabolism and intestinal microbiota diversity in mice

The potential effects of Komagataeibacter hansenii CGMCC 3917 cells on alcohol-induced liver injury and their probable mechanisms were investigated.