Visiting Hours Impact on Indoor to Outdoor Ratio of Fungi Concentration at Golestan University Hospital in Ahvaz, Iran

Prevalence of diseases originated from air pollution such as asthma and allergies, can be attributed to the bioaerosols. Bacteria and fungi are the main sources of hospital infections, which cause most of the diseases and mortality. The aim of this study was to determine the ratio of indoor to outdoor fungi concentration, the effect of population and people density on fungi concentration and the relationship between indoor and outdoor environment. In this study, three stations were used: outdoor of the hospital, general indoor and ICU wards of the Ahvaz hospital. These locations were chosen for sampling of the airborne fungi from October to December 2010. The samples were collected in 2 min, flow rate of 28.3 L/min using quick take (SKC Inc., PA, USA), based on the Andersen method.The three dominant culturable fungi genera in sampling stations were Aspergillus, Penicillium, and Cladosporium. The average of total culturable fungi concentration before visiting time was 365.8 CFU m-3 and increased to 578CFU m-3 after visiting time. The indoor to outdoor ratios (I/O) in ICU and internal for fungi before visit were 0.36 and 0.68, respectively. However, these ratios in ICU and internal station for fungi after visit were 0.78 and 0.99, respectively. Following the visit of the visitors, the concentration of fungi available indoor was conspicuously higher (even in the wards in which no visitors were allowed; e.g. ICU, etc). Hence the amount of indoor fungi was affected by the concentration of outdoor fungi and visitors crowd.

Volumetric Assessment of Airborne Indoor and Outdoor Fungi at Poultry and Cattle Houses in the Mazandaran Province, Iran

Volumetric Assessment of Airborne Indoor and Outdoor Fungi at Poultry and Cattle Houses in the Mazandaran Province, IranThe aim of this study was to assess the volume of airborne fungi in the indoor and outdoor environment of poultry and cattle houses in the Mazandaran Province in Iran. Indoor and outdoor air of twenty cattle houses and twenty-five poultry houses were sampled using a single-stage impactor, which draws air at 20 L min-1and impacts sampled material onto Petri plates containing malt extract agar. The plates were incubated at 30 °C for seven days, after which the resulting colonies were counted. The fungi were identified and counted microscopically and macroscopically. A total of 4,662 fungal colonies were isolated from 90 plates collected from indoor and outdoor air of cattle and poultry houses.Cladosporium(55.3 %), yeast (10.0 %), andAspergillus(9.4 %) were the most common findings. The concentration of airborne fungi in cattle and poultry houses ranged from 10 CFU m-3to 1700 CFU m-3in indoor and 10 CFU m-3to 2170 CFU m-3in outdoor environments.Cladosporiumhad the highest mean indoor (424.5 CFU m-3) and outdoor (449.7 CFU m-3) air concentration in the cattle houses. In the poultry houses, the highest mean concentrations were measured forCladosporium(551.0 CFU m-3) outdoors and yeast (440.7 CFU m-3) indoors. These levels might present an occupational risk, but threshold levels for these environments have yet to be established worldwide.