Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling

Acidobacteriota are widespread and often abundant in marine sediments, yet their metabolic and ecological properties are poorly understood. Here, we examined metabolisms and distributions of Acidobacteriota in marine sediments of Svalbard by functional predictions from metagenome-assembled genomes (MAGs), amplicon sequencing of 16S rRNA and dissimilatory sulfite reductase (dsrB) genes and transcripts, and gene expression analyses of tetrathionate-amended microcosms. Acidobacteriota were the second most abundant dsrB-harboring (averaging 13%) phylum after Desulfobacterota in Svalbard sediments, and represented 4% of dsrB transcripts on average. Meta-analysis of dsrAB datasets also showed Acidobacteriota dsrAB sequences are prominent in marine sediments worldwide, averaging 15% of all sequences analysed, and represent most of the previously unclassified dsrAB in marine sediments. We propose two new Acidobacteriota genera, Candidatus Sulfomarinibacter (class Thermoanaerobaculia, “subdivision 23”) and Ca. Polarisedimenticola (“subdivision 22”), with distinct genetic properties that may explain their distributions in biogeochemically distinct sediments. Ca. Sulfomarinibacter encode flexible respiratory routes, with potential for oxygen, nitrous oxide, metal-oxide, tetrathionate, sulfur and sulfite/sulfate respiration, and possibly sulfur disproportionation. Potential nutrients and energy include cellulose, proteins, cyanophycin, hydrogen, and acetate. A Ca. Polarisedimenticola MAG encodes various enzymes to degrade proteins, and to reduce oxygen, nitrate, sulfur/polysulfide and metal-oxides. 16S rRNA gene and transcript profiling of Svalbard sediments showed Ca. Sulfomarinibacter members were relatively abundant and transcriptionally active in sulfidic fjord sediments, while Ca. Polarisedimenticola members were more relatively abundant in metal-rich fjord sediments. Overall, we reveal various physiological features of uncultured marine Acidobacteriota that indicate fundamental roles in seafloor biogeochemical cycling.

Seasonal variations in the origin of river sediments (Baker River, Chile): A pre-requisite for climate and hydrological reconstructions

<p></p><div> <div> <div> </div> <div><img>Fjord sediments are increasingly recognized as high-resolution recorders of past climate and hydrological variability. Using them as such, however, requires a comprehensive understanding of the variables that affect their properties and accumulation rates. Here, we conduct a spatial and temporal study of sediment samples collected at the head of Martínez Channel (Chilean Patagonia, 48°S), to understand how the fjord’s sediments register changes in the hydrodynamics of Baker River, Chile's largest river in terms of mean annual discharge. We apply end-member modeling to particle-size distributions of: (i) river suspended sediments, (ii) surface sediments collected along a proximal-distal transect at the fjord head, and (iii) fjord sediments collected in a sequential sediment trap at 15-day resolution during two consecutive years. Results show that the river suspended sediments and fjord sediments are consistently composed of two grain-size subpopulations. The finest end member (EM<sub>1</sub>; mode 4.03 μm) reflects the meltwater contribution, which dominates in all but the winter season. The coarser end member (EM<sub>2</sub>; mode 18.7 μm) dominates in winter, when the meltwater contribution is reduced, and is associated to rainfall events. We propose that log(EM<sub>1</sub>/EM<sub>2</sub>) can be used to reconstruct temperature in the lower Baker River watershed (r = 0.81, p < 0.001). We also show that the fluxes of EM<sub>1</sub> and EM<sub>2</sub> provide quantitative estimates of baseflow (r = 0.82, p < 0.001) and quickflow (r = 0.90, p < 0.001), respectively. These results support the use of fjord sediments for quantitative reconstructions of climate and hydrological changes in glacierized watersheds.</div> </div> </div>