Inhibition of Recombinant Chicken Cystatin on Expression of Aflatoxin Biosynthesis Genes on Peanut

Purposes: Cystatins, as a kind of reversible cysteine proteases and potential inhibitor, have been found widely distributed in organisms and have potential capability of inhibiting fungi. Aflatoxins, toxic and extremely carcinogenic natural secondary metabolites, result in worldwide contamination. Procedures and Methods: Recombinant Chicken Cystatin was conducted to surface of peanut followed by inoculation withA. flavusEstimation of growth scores and detection of Aflatoxin by HPLC and relative quantification expression level of biosynthetic genesnor-1,Ver-1,omtandaflRvia Real-time PCR were investigated indifferent days culture peanuts after incubating for 8d. Results: Scores were observed and aflatoxin B1 was detected since 4d in control and 5d in treatment. All of four expression levels of treatment groups were lower than control, beginning at 5d later one day than control group. Conclusions: Inhibition function of Cystatins was observed markedly, and it may bring promising contribution in the respective of peanut storage.