Sanguibacter suaedae sp. nov., isolated from the root of Suaeda aralocaspica in north-west PR China

A bacterial strain, designated YZGR15T, was isolated from the root of an annual halophyte Suaeda aralocaspica, collected from the southern edge of the Gurbantunggut desert, north-west PR China. Cells of the isolate were Gram-stain-positive, facultatively anaerobic, irregular rods. Growth occurred at 4–42 °C (optimum, 30–37 °C), at pH 6.0–9.0 (optimum, pH 7.0–7.5) and in the presence of 0–9 % (w/v) NaCl (optimum, 2–5 %). Phylogenetic analysis using 16S rRNA gene sequences indicated that strain YZGR15T showed the highest sequence similarity to Sanguibacter keddieii (98.27 %), Sanguibacter antarcticus (98.20 %) and Sanguibacter inulinus (98.06 %). Results of genome analyses of strain YZGR15T indicated that the genome size was 3.16 Mb, with a genomic DNA G+C content of 71.9 mol%. Average nucleotide identity and digital DNA–DNA hybridization values between strain YZGR15Tand three type strains were in the range of 76.5–77.8 % and 20.0–22.2 %, respectively. Analysis of the cellular component of strain YZGR15T revealed that the primary fatty acids were anteiso-C15 : 0, C16 : 0, C14 : 0 and iso-C16 : 0 and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and two unidentified glycolipids. The cell-wall characteristic amino acids were glutamic acid, alanine and an unknown amino acid. The whole-cell sugars for the strain were mannose, ribose, rhamnose, glucose and an unidentified sugar. The predominant respiratory quinone was MK-9(H4). Based on the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain YZGR15T represents a novel species of the genus Sanguibacter , for which the name Sanguibacter suaedae sp. nov. is proposed. The type strain is YZGR15T (=CGMCC 1.18691T=KCTC 49659T)

Comparative Analysis of Two Methods for Conduction Transfer Functions of Building Constructions

Conduction transfer function (CTF) is widely used to calculate conduction heat transfer in building cooling/heating load and energy calculations. It can conveniently fit into any load and energy calculation techniques to perform conduction heat transfer calculations. There are two popular methods, direct root-finding (DRF) method and frequency-domain regression (FDR) method to calculate CTF coefficients. The limitation of methodology possibly results in imprecise or false CTF coefficients. This paper analyzes the errors of two methods to the material properties of a multilayer heavyweight building construction. The results show that the calculation error of DRF method becomes increasing as the wall characteristic parameter becomes increasing. The maximal error even reaches almost 100%. However, the calculation error of FDR method always remains within 1% no matter how the wall characteristic parameters are varied. Thus, FDR method is more robust and reliable than DRF method.

Errors Analysis of Calculation Methods for Conduction Transfer Functions of Building Constructions

Conduction transfer function (CTF) is widely used to calculate conduction heat transfer in building cooling/heating load and energy calculations. It can conveniently fit into any load and energy calculation techniques to perform conduction heat transfer calculations. There are two popular methods, state-space (SS) method and frequency-domain regression (FDR) method to calculate CTF coefficients. The limitation of methodology possibly results in imprecise or false CTF coefficients. This paper analyzes the errors of two methods to the material properties of a single-layer and a multilayer heavyweight building construction. The results show that the calculation error of SS method becomes increasing as the wall characteristic parameter becomes increasing. The maximal error even reaches almost 100%. However, the calculation error of FDR method always remains within 1% no matter how the wall characteristic parameters are varied. Thus, FDR method is more robust and reliable than SS method.