Assessing the impact of nonspecific binding on oligonucleotide bioanalysis

Aim: Accurate and reliable quantification of oligonucleotides can be difficult, which has led to an increased focus on bioanalytical methods for more robust analyses. Recent advances toward mitigating sample losses on liquid chromatography (LC) systems have produced recovery advantages for oligonucleotide separations. Results & methodology: LC instruments and columns constructed from MP35N metal alloy and stainless steel columns were compared against LC hardware modified with hybrid inorganic-organic silica surfaces. Designed to minimize metal-analyte adsorption, these surfaces demonstrated a 73% increase in 25-mer phosphorothioate oligonucleotide recovery using ion-pairing reversed-phase LC versus standard LC surfaces, most particularly upon initial use. Conclusion: Hybrid silica chromatographic surfaces improve the performance, detection limits and reproducibility of oligonucleotide bioanalytical assays.

Extraction of Silica from Rice Husk and Bamboo Leaves and its Effect on the Ceramic Body Glazing Process

Rice husk and bamboo leaves are types of agricultural wastes that can easily be found around the world. Rice husk contains over 60 % of organic silica while silica bamboo leaves extract consist of more than 70 % silica content. Parameters such as concentration of acid and weight percentage of rice husk and bamboo leaves used during acid leaching method were also compared. In this paper, citric acid (C6H8O7) was used for production of silica from rice husk and bamboo leaves. Samples were characterized using X-Ray Fluorescence (XRF) and Field Emission Scanning Electron Microscope Energy Dispersive X-Ray spectroscopy (FESEM / EDX). The performance of glaze on ceramic will be analyzed by undergoes hardness testing. The result indicates that highest percentage silica of rice husk and bamboo leaves after leaching was on 1.0M.

Pengaruh pupuk biosilika terhadap pertumbuhan, hasil, dan kekerasan biji tanaman hanjeli (Coix lacryma-jobi L.) varietas batu dan pulut

Sari. Tujuan dari penelitian adalah untuk mengetahui dosis pupuk silika organik yang tepat yang memberikan pengaruh terbaik terhadap pertumbuhan, hasil, dan kekerasan biji hanjeli (Coix lacryma-jobi L.). Penelitian dilaksanakan di Kebun Percobaan Fakultas Pertanian UNPAD, Ciparanje, Jatinangor, sejak bulan Desember 2015 sampai Mei 2016. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK) yang terdiri dari 12 perlakuan dan 4 ulangan. Perlakuan terdiri enam taraf dosis pupuk biosilika (arang kulit biji hanjeli pulut yang mengandung 12% SiO2) masing-masing per ha adalah 0 kg; 150 kg; 300 kg; 450 kg; 600 dan 750 kg pada dua jenis hanjeli batu (var. Stenocarpa) dan pulut (var. Mayuen). Data dianalisis menggunakan Sidik Ragam dengan Uji F pada taraf nyata 5%, sementara nilai beda dengan Uji Duncan pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa pupuk biosilika berpengaruh terhadap jumlah malai per rumpun, indeks panen, dan kekerasan biji hanjeli pada kandungan silika tanah tinggi. Dosis 150 kg/ha dan 750 kg/ha pada hanjeli batu berpengaruh terhadap jumlah malai per rumpun dibandingkan kontrol. Dosis 600 kg/ha memberikan IP terbaik dibandingkan kontrol pada hanjeli pulut. Semua dosis silika berpengaruh terhadap kekerasan biji hanjeli batu dibandingkan kontrol. Kata Kunci: Hanjeli, Biosilika, Pertumbuhan dan hasil, Kekerasan biji Abstract. The research was conducted to determine the dosage of organic silica that can give the best effect to the growth, yield, and seed hardness of Job’s tears. This research was conducted from Desember 2015 to May 2016 at Ciparanje Experiment Station, Jatinangor, West Java in Faculty of Agriculture, Padjadjaran University. The experimental design used Randomized Block Design of twelve treatments and four replication. Treatments consisted of biosilica fertilizer (kg/ha): 0; 150; 300; 450; 600 and 750; that given to two varieties of job’s tears: stenocarpa and mayuen. Data were analyzed by Anova (F test) at 5% significance level, then tested by Duncan test at 5% significance level. The results showed that the biosilica fertilizer influenced panicle number, harvest index; and seed hardness. Dosage of 150 kg/ha and 750 kg/ha biosilica affected panicle number on Stenocarpa. Dosage of 600 kg/ha gave the better harvest index than no silica fertilizer on Mayuen. All of silica dosage gave higher seed hardness than no silica fertilizer on Stenocarpa.Keywords: Job’s tears, Growth and yield, Seed hardness