Single cell chromatin accessibility of the developmental cephalochordate

The phylum chordata are composed of three groups: vertebrata, tunicate and cephalochordata. Single cell developmental atlas for typical species in vertebrata (mouse, zebrafish, western frog, worm) and tunicate (sea squirts) has been constructed recently. However, the single cell resolution atlas for lancelet, a living proxy of vertebrate ancestors, has not been achieved yet. Here, we profiled more than 57 thousand cells during the development of florida lancelet (Branchiostoma floridae), covering important processes including embryogenesis, organogenesis and metamorphosis. We identified stage and cluster specific regulatory elements. Additionally, we revealed the regulatory codes underlying functional specification and lineage commitment. Based on epigenetic features, we constructed the developmental trajectory for lancelet, elucidating how cell fates were established progressively. Overall, our study provides, by far, the first single cell regulatory landscape of amphioxus, which could help us to understand the heterogeneity and complexity of lancet development at single cell resolution and throw light upon the great transition from simple chordate ancestor to modern vertebrates with amazing diversity and endless forms.

Marine Ligands of the Pregnane X Receptor (PXR): An Overview

Pregnane X Receptor (PXR) is a ligand-activated transcription factor which binds many structurally different molecules. The receptor is able to regulate the expression of a wide array of genes and is involved in cancer and different key physiological processes such as the metabolism of drugs/xenobiotics and endogenous compounds including lipids and carbohydrates, and inflammation. Algae, sponges, sea squirts, and other marine organisms are some of the species from which structurally new molecules have been isolated that have been subsequently identified in recent decades as ligands for PXR. The therapeutic potential of these natural compounds is promising in different areas and has recently resulted in the registration of trabectedin by the FDA as a novel antineoplastic drug. Apart from being potentially novel drugs, these compounds can also serve as models for the development of new molecules with improved activity. The aim of this review is to succinctly summarize the currently known natural molecules isolated from marine organisms with a proven ability to interact with PXR.

Nephromyces represents a diverse and novel lineage of the Apicomplexa that has retained apicoplasts

A most interesting exception within the parasitic Apicomplexa is Nephromyces, an extracellular, probably mutualistic, endosymbiont found living inside molgulid ascidian tunicates (i.e., sea squirts). Even though Nephromyces is now known to be an apicomplexan, many other questions about its nature remain unanswered. To gain further insights into the biology and evolutionary history of this unusual apicomplexan, we aimed to (1) find the precise phylogenetic position of Nephromyces within the Apicomplexa, (2) search for the apicoplast genome of Nephromyces, and (3) infer the major metabolic pathways in the apicoplast of Nephromyces. To do this, we sequenced a metagenome and a metatranscriptome from the molgulid renal sac, the specialized habitat where Nephromyces thrives. Our phylogenetic analyses of conserved nucleus-encoded genes robustly suggest that Nephromyces is a novel lineage sister to the Hematozoa, which comprises both the Haemosporidia (e.g., Plasmodium) and the Piroplasmida (e.g., Babesia and Theileria). Furthermore, a survey of the renal sac metagenome revealed 13 small contigs that closely resemble the genomes of the non-photosynthetic reduced plastids, or apicoplasts, of other apicomplexans. We show that these apicoplast genomes correspond to a diverse set of most closely related but genetically divergent Nephromyces lineages that co-inhabit a single tunicate host. In addition, the apicoplast of Nephromyces appears to have retained all biosynthetic pathways inferred to have been ancestral to parasitic apicomplexans. Our results shed light on the evolutionary history of the only probably mutualistic apicomplexan known, Nephromyces, and provide context for a better understanding of its life style and intricate symbiosis.