In view of the existing of low frequency hydrophone in signal detection problems such as low sensitivity, frequency range is small, In this paper, application of PVDF piezoelectric film as sensitive material of low frequency hydrophone, use its advantages as fast response, high sensitivity, good mechanical properties, and so on [1], designed a kind of low frequency hydrophone, experiments are carried out to study the detection of low frequency signal. This paper introduces the structure, material and size of the hydrophone, and analyzes the experimental data. Experiments show that the sensor stability and high sensitivity, can effectively detect the low frequency acoustic wave in the water.