Single cell RNA-seq reveals immunosuppressive gastric stem-like cancer cells as a poor prognostic factor

SUMMARYThe tumor microenvironment is characterized by high cellular heterogeneity and a complex network of cell-cell communications. Analysis at the single cell level is required to dissect this complexity. Here we apply single-cell full-length transcriptome sequencing to analyze 3443 cells from paired normal-adjacent and tumor tissues of 15 gastric cancer patients with different histological profiles. Among the epithelial cells profiled, we discovered subsets of proliferative stem-like cells with upregulated pro-tumor pathways and that were associated with poorer prognosis. Stem-like cell enriched tissues also show distinct T cell populations with proportionally more TIM3+ CD8+ cells, while non-enriched tissues had proportionally more KLRC1+ CD8+ cells, offering opportunities for checkpoint inhibitor therapy. In silico predictions of ligand-receptor interactions revealed immune-suppressive interactions between these stem-like cells and immune cells via interacting pairs such as Nectin-2(CD112)-TIGIT, Galectin-9-TIM3 and CXCL16-CXCR6. Using immunohistochemistry, we found CD8+ T cells expressing TIGIT in close proximity to stem-like cells expressing NECTIN2.

Girl power! Femvertising jako nowy trend w komunikacji marketingowej. Analiza zjawiska na podstawie wybranych kampanii

Girl power! Femvertising as a new trend in marketing communications. Analysis based on selected campaignsBrands are a litmus paper of cultural and social changes that happen all around us. Not only must they follow the trends, they have to create them and adapt their personality and identity accordingly. One of vital questions are growing ambitions of women — and with them a frustration that has arisen from facing a reality full of limiting stereotypes. Third-wave feminism grows stronger and marketing, inherent in the social reality, forecasts new tendencies and proposes a new type of message. That’s how the femvertising was born — marketing communication aimed at women, which presents them as separate from stereotyped social roles. It focuses on real problems women face when trying to pursue self-development. The article presents the results of semiotic analysis of marketing messages of brands: Dove and Always, based on a model which allows to analyze the message on the several interconnected levels of meaning. The purpose of this text is to verify what image of the world and the feminity results from the femvertising type of communication, and what challenges the femvertising faces as a fairly new type of communication.