Improving the Effectiveness of Drug Therapy for Hypertension in Men with Combined Use of Low Intensity Uhf Physiotherapy

120 men (51,5±2,5 years old) with arterial hypertension stage П-Ш were examined. From the first days of stay of these patients in the clinic, a physical therapy low-intensity UHF radiation frequency of 1000 MHz with a power of 0.1 μw was included in the complex therapy. On the background of a drug therapy, there was a statistically significant increase of NO level by 12,4% (p=0,039) and ACE by 11,8% (p=0,05). A month later after treatment, the authors observed a decrease in the concentration of the soluble form of receptors of the 1st type to AHT-II by 22,4% (p=0,017). In the group of patients who received additional physical therapy, there was a decrease in renin activity by 19,3% (p=0,021), RAHT-Π by 18,8% (p=0,023). A month later, in these patients there was a further decrease in the concentration of RAHT-II by 52,8% (p=0,001), ACE by 12,3% (p=0,037), AHT-II by 13,4% (p=0,033), an endothelin level by 10,7% (p=0,051) and hsCRP by 13,2% (p=0,044). An increase in the concentration of NO level by 11.4% (p=0.05) was revealed. Thus, the use of low-intensity UHF physiotherapy in treatment of hypertension contributes to a reduction of endo-thelial dysfunction and vasopressor activity mechanisms with preservation of the achieved effects within one month after discharge the patient from hospital.

A novel angiotensin I isolated from an elasmobranch fish

ABSTRACT It is believed that the renin-angiotensin system evolved initially in primitive bony fishes and is absent from elasmobranchs. We have isolated angiotensin I from the incubates of plasma and kidney extracts of an elasmobranch fish, Triakis scyllia, using eel vasopressor activity as an assay system. Its sequence was determined to be H-Asn-Arg-Pro-Tyr-Ile-His-ProPhe-Gln-Leu-OH. Dogfish angiotensin I is teleost-like because of an asparagine residue at position 1 but it is mammalian-like because of an isoleucine residue at position 5. The unique and most important substitution in dogfish angiotensin I is a proline residue at position 3 which may cause significant changes in its tertiary structure. A glutamine residue at position 9 is also unique among all angiotensin Is sequenced to date. Dogfish angiotensin I is more potent than rat angiotensin I in its vasopressor activity in the dogfish but the relationship is reversed in the rat. Thus angiotensin receptors as well as the hormone molecules appear to have evolved during vertebrate phylogeny. Our findings establish the elasmobranch renin-angiotensin system and support the hypothesis that the renin-angiotensin system is a phylogenetically old hormonal system which plays important roles in cardiovascular and fluid homeostasis. Journal of Endocrinology (1993) 139, 281–285

Generation of angiotensin II from human plasma by tissue kallikrein

1. Human plasma was incubated with tissue kallikrein from porcine pancreas, dialysed to obtain a fraction with a molecular mass < 10 kDa and further purified by reverse-phase chromatography. 2. Vasopressor activity in the fractions obtained was tested in the isolated perfused rat kidney. 3. In one fraction a strong vasopressor action was found, which was blocked by saralasin and by an angiotensin II antibody. 4. Aprotinin inhibited the formation of vasopressor substances by tissue kallikrein. 5. U.v.-laser desorption/ionization mass spectrometry revealed a molecular mass of 1046 Da in the purified active fraction. 6. It is concluded that tissue kallikrein forms not only kinins, but also angiotensin II, from human plasma under physiological conditions.

Effect of vasopressin on atrial natriuretic peptide release and renal function in dogs

To assess the effect of arginine vasopressin (AVP) and 1-deamino-8-D-AVP (DDAVP) on atrial natriuretic peptide (ANP) release, renal water and electrolyte excretion, and cardiovascular function, AVP and DDAVP were administered at a dose of 10 ng.kg-1.min-1 iv for 30 min into anesthetized dogs receiving saline infusion at a rate of 1 ml.kg-1.min-1 (n = 12). In the control study, saline was infused alone (n = 6). AVP potentiated the plasma ANP response to an increase in plasma volume produced by saline infusion, increased mean arterial blood pressure (MAP), and exaggerated the natriuresis and kaliuresis. DDAVP did not potentiate the increase in plasma ANP but enhanced the natriuresis without any rise in MAP. Saline alone increased plasma ANP as well as sodium and potassium excretion with no changes in MAP. Inulin and p-aminohippuric acid clearances did not change during these studies. The results suggest that in hydrated dogs, AVP may increase ANP release and arterial blood pressure via the vasopressor activity of AVP and potentiate the natriuresis and kaliuresis, but the increased ANP may play little role in the natriuresis.