Global Identification of C2 Domain Contain Proteins And Characterization Of Genetic Variations For Involved Abiotic Stress Tolerance In Rice (Oryza sativa L.)

BackgroundC2DPs (C2 domain contain proteins) have been identified in different genomes that contain single or multiple C2 domains in their C or N-terminal, it possesses higher functional activity in the cell membrane between the cytoplasm and nucleus. Despite the identification of MCTPs and NTMC2s in rice, Arabidopsis, and cotton in a previous study, however, the C2DP gene family in rice has not been comprehensively studied, and the role of the C2DP gene in rice in response to abiotic stress is unclear.ResultsIn this study, we identified 82 C2DPs in the rice genome and divided them into seven groups through phylogenetic analysis. Synteny analysis revealed that duplication events were either exhibited within the genome of rice or between the genome of rice and other species. Through the analysis of cis-acting elements in promoters, expression profiles, and qRT-PCR results, the functions of OsC2DPs were found to be widely expressed in diverse tissues and were extensively involved in phytohormones and abiotic stress in rice. Prediction of the miRNA targets of OsC2DPs revealed that some of the homolog genes were regulated by consistent miRNAs and may carry out redundancy function. Notably, OsC2DP50/51/52 as a co-tandem duplication exhibited similar expression variations and involved the coincident miRNA-regulation pathway. Moreover, the results of SNP genotyping and haplotype analysis revealed that OsC2DP17, OsC2DP29, and OsC2DP49 possessed diverse haplotypes for impacted cold tolerance owing to genomic variations.ConclusionsThese findings provide a comprehensive sight for characterized OsC2DPs in rice and their roles for abiotic stress. Further, the genetic variation supports the theoretical reference for molecular breeding in rice.

The Study of the Managing Node Redundancy of Real-Time Industrial Ethernet POWERLINK

This paper analyses the master station redundancy technology in the POWERLINK network. Through the monitoring network and data refresh to achieve the master station redundancy function. The Managing Node redundancy ensures the POWERLINK cycle production continuance after the failure of the current master station, the switch-over time (recovery time) of the POWERLINK system is in the two POWERLINK cycle time at least. That ensures a very fast restoring of normal operation without any downtime for the control system.