This study investigates the degradation of superheater tubes that has been in service for very long time. It also discusses the degradation effects on tube remaining life predictions. The tubes, utilized in this study, belong to an industrial boiler that has been in service for 232 000 h in a petrochemical plant and generate steam at 47 barg and 410 °C. Outcomes of this study will contribute to better understanding and development of scientific procedures to make reasonable estimate of tube remaining life considering the tubes aging while in service. This is beneficial in preventing failures and forced plant shutdown when life is consumed. It also helps industry to avoid capital expenditures on premature replacements of boiler tubes that can still serve longer time. In this study, comparison is made between short-term life predictions and actual properties found after about 26.5 years of service. The tubes condition is assessed by metallographic, mechanical and stress rupture testing. According to the results, it is found that prolonged service degradation has strong effects on remaining life predictions. Both effects that would lead to overestimating or underestimating tube remaining life were found if existing procedures are used without consideration for material degradation during service.