Histological study of the liver and biochemistry of the blood of Wistar rats following ligature of right hepatic duct

PURPOSE: To observe the histological alterations in the liver and biochemistry in the blood that can happen in Wistar rat, after the ligature of right hepatic duct. METHODS: In this study were used rats (n=46) of Wistar pedigree. The animal groups (n=46) were distributed in 6 experimented sub-groups (n=6). It was held a ligature surgery of the right hepatic duct and euthanasia in 7, 14, 21, 28, 60 and 90 days and the biochemistry control group (n=10), that animals had 2ml of their blood taken by cardiac puncture for biochemistry study with value analyses of bilirubins, transaminasis, lactic desidrogenasis, alkaline phophatase and gamma-glutamil-transferase. Given the expected time of each group, the animals were submitted to anesthesia procedure and cavity re-opening, being held intra-cardiac puncture and with 2ml blood collected for biochemistry analyses. It was proceeded the liver resection, being the liver putted in formol solution to 10% for a period of 24 hours and taken to the histology. RESULTS: It was not possible to identify results that express significant differences as the existence of alterations histological and biochemistrily between the different groups. CONCLUSION: At the end of the study, it was not possible to identify histological and biochemistrily alterations that express significant differences between livers of the animals from the right linked hepatic duct and the animals of the control group.

GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE): II. ELECTROPHORETIC BANDING PATTERNS OF MIDGUT ALKALINE PHOSPHATASE

The digestive section of the midgut of Glossina morsitans morsitans Westwood contains a phosphatase with a pH optimum of approximately 9.2 and with low substrate specificity; the enzyme was classified as an alkaline phosphatase (E.C. 3.1.3.1).Polyacrylamide gel (6%) electrophoresis (at pH 8.9) of the digestive portion of the midguts of adult G. morsitans morsitans revealed three alkaline phophatase phenotypes. Midgut phosphatase was postulated to be under control of a single locus (designated alkph) with two alleles. Gene frequencies were in Hardy-Weinberg equilibrium in two laboratory populations while a third, highly inbred population had only one phenotype. Phenotype frequencies were not significantly different among females of various ages from the Edmonton colony. Breeding experiments provided direct evidence for single locus control of midgut phosphatase.

Serum alkaline phosphatase: normal values by sex and age.

We report here the normal range of serum alkaline phophatase activity as measured by the method proposed by Hausamen et al. [Clin. Chim. Acta 15, 241 (1967)] with a much larger sample size than used in previous investigations. In the statistical analysis the sample population is subdivided by sex and age, two variables which are known to influence enzyme activity. No statistically significant influence of blood type on enzyme activity was observed. The normal range of enzyme activity is reported in percentiles.