The objective of this research is to determine whether there is a difference in the Ca, P, Fe, F, Mg, and Sr concentrations in teeth from children with and without cystic fibrosis (CF) as a function of age, sex of the child, maternal smoking habit, maternal drinking habit, and fluoridation of water supply. The nuclear analytical methods used were Proton-Induced X-Ray Emission (PIXE) and Proton-Induced Gamma Emission (PIGE). The findings are: (1) the concentration of Mg is higher in the teeth of children with cystic fibrosis who took antibiotics other than tetracycline (CF+NT) than in non-cystic fibrosis (NCF) teeth for the “female” category; (2) P concentration is less in (CF+NT) teeth for the “age greater than ten” group; (3) Fe concentration is greater for females in the NCF group; (4) Ca and Fe concentrations are lower for CF than for NCF in the “mother smokes” group; (5) F and Sr concentrations for CF+NT are higher for the “mother drinks” group than for the “mother does not drink” group; (6) “mother smokes” shows a depletion of Ca and Fe compared to “mother does not smoke” for CF+NT. (7) “fluoridation of water supply” shows an increase in F, except for NCF teeth.