scholarly journals A Versatile AIE Fluorogen with Selective Reactivity to Primary Amines for Monitoring Amination, Protein Labeling and Mitochondrial Staining

2021 ◽  
Author(s):  
Xinyuan He ◽  
Huilin Xie ◽  
Lianrui Hu ◽  
Pengchao Liu ◽  
Changhuo Xu ◽  
...  
Keyword(s):  
Fast Response ◽  
Molecular Engineering ◽  
Protein Labeling ◽  
Primary Amines ◽  
Probe Design ◽  
Potential Applications ◽  
Good Biocompatibility ◽  
Selective Reactivity ◽  
Mitochondrial Staining ◽  
Wide Potential

Specific bioconjugation for native primary amines is highly valuable for both chemistry and biomedical research. Despite all the efforts, scientists lack a proper strategy to achieve high selectivity for primary amines, not to mention the requirement of fast response for real applications. Herein, in this work, we report a chromone-based aggregation-induced emission (AIE) fluorogen called CMVMN as a self-reporting bioconjugation reagent for selective primary amine identification, and its applications for monitoring bioprocesses of amination and protein labeling. CMVMN is AIE-active and is capable of solid-state sensing. Thus, its electrospun films are manufactured for visualization of amine diffusion and leakage process. CMVMN also shows good biocompatibility and potential mitochondria-staining ability, which provides new insight for organelle-staining probe design. Combined with its facile synthesis and good reversibility, CMVMN not only shows wide potential applications in biology, but also offers new possibilities for molecular engineering.

scholarly journals Graphene Integrated Hydrogels Based Biomaterials in Photothermal Biomedicine

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 906
Author(s):  
Le Minh Tu Phan ◽  
Thuy Anh Thu Vo ◽  
Thi Xoan Hoang ◽  
Sungbo Cho
Keyword(s):  
Photothermal Therapy ◽  
Biomedical Applications ◽  
High Light ◽  
Conversion Materials ◽  
Potential Applications ◽  
Good Biocompatibility ◽  
Biomedical Field ◽  
Hydrogel Composites ◽  
Material Fabrication ◽  
Biomedical Fields

Recently, photothermal therapy (PTT) has emerged as one of the most promising biomedical strategies for different areas in the biomedical field owing to its superior advantages, such as being noninvasive, target-specific and having fewer side effects. Graphene-based hydrogels (GGels), which have excellent mechanical and optical properties, high light-to-heat conversion efficiency and good biocompatibility, have been intensively exploited as potential photothermal conversion materials. This comprehensive review summarizes the current development of graphene-integrated hydrogel composites and their application in photothermal biomedicine. The latest advances in the synthesis strategies, unique properties and potential applications of photothermal-responsive GGel nanocomposites in biomedical fields are introduced in detail. This review aims to provide a better understanding of the current progress in GGel material fabrication, photothermal properties and potential PTT-based biomedical applications, thereby aiding in more research efforts to facilitate the further advancement of photothermal biomedicine.

scholarly journals Cryo-EM structures of an insecticidal Bt toxin reveal its mechanism of action on the membrane

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Matthew J. Byrne ◽  
Matthew G. Iadanza ◽  
Marcos Arribas Perez ◽  
Daniel P. Maskell ◽  
Rachel M. George ◽  
...  
Keyword(s):  
Functional Data ◽  
Insect Pests ◽  
Economic Cost ◽  
Commercial Agriculture ◽  
Efficient Manner ◽  
Bt Toxin ◽  
Genes Encoding ◽  
Potential Applications ◽  
Chemical Pesticides ◽  
Wide Potential

AbstractInsect pests are a major cause of crop losses worldwide, with an estimated economic cost of $470 billion annually. Biotechnological tools have been introduced to control such insects without the need for chemical pesticides; for instance, the development of transgenic plants harbouring genes encoding insecticidal proteins. The Vip3 (vegetative insecticidal protein 3) family proteins from Bacillus thuringiensis convey toxicity to species within the Lepidoptera, and have wide potential applications in commercial agriculture. Vip3 proteins are proposed to exert their insecticidal activity through pore formation, though to date there is no mechanistic description of how this occurs on the membrane. Here we present cryo-EM structures of a Vip3 family toxin in both inactive and activated forms in conjunction with structural and functional data on toxin–membrane interactions. Together these data demonstrate that activated Vip3Bc1 complex is able to insert into membranes in a highly efficient manner, indicating that receptor binding is the likely driver of Vip3 specificity.

scholarly journals Laser-Induced Deposition of Plasmonic Ag and Pt Nanoparticles, and Periodic Arrays

Materials ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 10
Author(s):  
Daria V. Mamonova ◽  
Anna A. Vasileva ◽  
Yuri V. Petrov ◽  
Denis V. Danilov ◽  
Ilya E. Kolesnikov ◽  
...  
Keyword(s):  
Substrate Surface ◽  
Pt Nanoparticles ◽  
Single Step ◽  
X Ray Diffraction ◽  
Ag Nps ◽  
X Ray ◽  
Periodic Arrays ◽  
Potential Applications ◽  
Surface Decoration ◽  
Wide Potential

Surfaces functionalized with metal nanoparticles (NPs) are of great interest due to their wide potential applications in sensing, biomedicine, nanophotonics, etc. However, the precisely controllable decoration with plasmonic nanoparticles requires sophisticated techniques that are often multistep and complex. Here, we present a laser-induced deposition (LID) approach allowing for single-step surface decoration with NPs of controllable composition, morphology, and spatial distribution. The formation of Ag, Pt, and mixed Ag-Pt nanoparticles on a substrate surface was successfully demonstrated as a result of the LID process from commercially available precursors. The deposited nanoparticles were characterized with SEM, TEM, EDX, X-ray diffraction, and UV-VIS absorption spectroscopy, which confirmed the formation of crystalline nanoparticles of Pt (3–5 nm) and Ag (ca. 100 nm) with plasmonic properties. The advantageous features of the LID process allow us to demonstrate the spatially selective deposition of plasmonic NPs in a laser interference pattern, and thereby, the formation of periodic arrays of Ag NPs forming diffraction grating

Circularly polarized luminescence from oriented polymer films doped with a tetraphenylethylene-based conjugated oligomer

2021 ◽  
Author(s):  
Wan Zhang ◽  
Baining Ni ◽  
Hongkun Li ◽  
Yonggang Yang ◽  
Yongfang Li ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Polymer Films ◽  
High Quantum Yield ◽  
Oriented Polymer ◽  
Active Materials ◽  
Circularly Polarized ◽  
Circularly Polarized Luminescence ◽  
Polarized Luminescence ◽  
Potential Applications ◽  
Wide Potential

Circularly polarized luminescence (CPL) materials have attracted increasing interest due to their wide potential applications. However, achieving CPL-active materials with both large dissymmetry factor (glum) and high quantum yield (F)...

First Unsteady Pressure Measurements With a Fast Response Cooled Total Pressure Probe in High Temperature Gas Turbine Environments

2010 ◽  
Author(s):  
Mehmet Mersinligil ◽  
Jean-Franc¸ois Brouckaert ◽  
Julien Desset
Keyword(s):  
High Pressure ◽  
Pressure Sensor ◽  
Total Pressure ◽  
Fast Response ◽  
Design Stage ◽  
Absolute Pressure ◽  
Pressure Probe ◽  
Probe Design ◽  
Flame Tube ◽  
Blade Passing Frequency

This paper presents the first experimental engine and test rig results obtained from a fast response cooled total pressure probe. The first objective of the probe design was to favor continuous immersion of the probe into the engine to obtain time series of pressure with a high bandwidth and therefore statistically representative average fluctuations at the blade passing frequency. The probe is water cooled by a high pressure cooling system and uses a conventional piezo-resistive pressure sensor which yields therefore both time-averaged and time-resolved pressures. The initial design target was to gain the capability of performing measurements at the temperature conditions typically found at high pressure turbine exit (1100–1400K) with a bandwidth of at least 40kHz and in the long term at combustor exit (2000K or higher). The probe was first traversed at the turbine exit of a Rolls-Royce Viper turbojet engine, at exhaust temperatures around 750 °C and absolute pressure of 2.1bars. The probe was able to resolve the high blade passing frequency (≈23kHz) and several harmonics up to 100kHz. Besides the average total pressure distributions from the radial traverses, phase-locked averages and random unsteadiness are presented. The probe was also used in a virtual three-hole mode yielding unsteady yaw angle, static pressure and Mach number. The same probe was used for measurements in a Rolls-Royce intermediate pressure burner rig. Traverses were performed inside the flame tube of a kerosene burner at temperatures above 1600 °C. The probe successfully measured the total pressure distribution in the flame tube and typical frequencies of combustion instabilities were identified during rumble conditions. The cooling performance of the probe is compared to estimations at the design stage and found to be in good agreement. The frequency response of the probe is compared to cold shock tube results and a significant increase in the natural frequency of the line-cavity system formed by the conduction cooled screen in front of the miniature pressure sensor were observed.

Design and Testing of a Miniaturized Five-Hole Fast Response Pressure Probe With Large Frequency Bandwidth and High Angular Sensitivity

2019 ◽  
Vol 141 (10) ◽  
Author(s):  
Elissavet Boufidi ◽  
Marco Alati ◽  
Fabrizio Fontaneto ◽  
Sergio Lavagnoli
Keyword(s):  
Dynamic Response ◽  
Three Dimensional ◽  
Experimental Tests ◽  
Fast Response ◽  
Pressure Probe ◽  
Probe Design ◽  
Directional Sensitivity ◽  
Angular Sensitivity ◽  
Numerical Predictions ◽  
Response Pressure

Abstract A miniaturized five-hole fast response pressure probe is presented, and the methods for the aerodynamic design and performance characterization are explained in detail. The probe design is aimed for three-dimensional (3D) time-resolved measurements in turbomachinery flows, therefore requiring high frequency response and directional sensitivity. It features five encapsulated piezoresistive pressure transducers, recessed inside the probe hemispherical head. Theoretical and numerical analyses are carried out to estimate the dynamic response of the pressure tap line-cavity systems and to investigate unsteady effects that can influence the pressure readings. A prototype is manufactured and submitted to experimental tests that demonstrate performance in line with the theoretical and numerical predictions of the dynamic response: the natural frequency of the central and lateral taps extends to 200 and 25 kHz, respectively. An aerodynamic calibration is also performed at different Reynolds and Mach numbers. The probe geometry offers a good angular sensitivity in a ± 30 deg incidence range, while a frequency analysis reveals the presence of pressure oscillations related to vortex shedding at large angles of attack.

scholarly journals Electrospun polyvinyl alcohol-honey nanofibers

2013 ◽  
Vol 17 (5) ◽  
pp. 1549-1550 ◽  
Author(s):  
Ping Wang ◽  
Ji-Huan He
Keyword(s):  
Polyvinyl Alcohol ◽  
Environmentally Friendly ◽  
Potential Applications ◽  
Wide Potential

This paper sugeests a method for fabrication of polyvinyl alcohol-honey nanofibers by electrospinning. Polyvinyl alcohol and honey are all biocompatible and environmentally friendly materials. This combination will lead to wide potential applications in various engineering fields.

scholarly journals An Electrochemical Sensor Based on a Nitrogen-Doped Carbon Material and PEI Composites for Sensitive Detection of 4-Nitrophenol

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 86
Author(s):  
Xue Nie ◽  
Peihong Deng ◽  
Haiyan Wang ◽  
Yougen Tang
Keyword(s):  
Electrochemical Sensor ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Environmental Water ◽  
Fast Response ◽  
Nitrogen Doped ◽  
Potential Applications ◽  
Good Repeatability ◽  
Nitrogen Doped Carbon ◽  
Optimized Conditions

A glassy carbon electrode (GCE) was modified with nitrogen-doped carbon materials (NC) and polyethyleneimine (PEI) composites to design an electrochemical sensor for detecting 4-nitrophenol (4-NP). The NC materials were prepared by a simple and economical method through the condensation and carbonization of formamide. The NC materials were dispersed in a polyethyleneimine (PEI) solution easily. Due to the excellent properties of NC and PEI as well as their synergistic effect, the electrochemical reduction of the 4-NP on the surface of the NC–PEI composite modified electrode was effectively enhanced. Under the optimized conditions, at 0.06–10 μM and 10–100 μM concentration ranges, the NC–PEI/GCE sensor shows a linear response to 4-NP, and the detection limit is 0.01 μM (the signal-to-noise ratio is three). The reliability of the sensor for the detection of 4-NP in environmental water samples was successfully evaluated. In addition, the sensor has many advantages, including simple preparation, fast response, high sensitivity and good repeatability. It may be helpful for potential applications in detecting other targets.

scholarly journals A Fast-Response Automated Gas Equilibrator (FaRAGE) for continuous in situ measurement of CH<sub>4</sub> and CO<sub>2</sub> dissolved in water

2020 ◽  
Vol 24 (7) ◽  
pp. 3871-3880 ◽  
Author(s):  
Shangbin Xiao ◽  
Liu Liu ◽  
Wei Wang ◽  
Andreas Lorke ◽  
Jason Woodhouse ◽  
...  
Keyword(s):  
Response Time ◽  
Greenhouse Gas ◽  
Three Dimensional ◽  
Fast Response ◽  
In Situ Measurement ◽  
Inland Waters ◽  
Gas Analyzer ◽  
Potential Applications ◽  
Carbon Dioxide Co2

Abstract. Biogenic greenhouse gas emissions, e.g., of methane (CH4) and carbon dioxide (CO2) from inland waters, contribute substantially to global warming. In aquatic systems, dissolved greenhouse gases are highly heterogeneous in both space and time. To better understand the biological and physical processes that affect sources and sinks of both CH4 and CO2, their dissolved concentrations need to be measured with high spatial and temporal resolution. To achieve this goal, we developed the Fast-Response Automated Gas Equilibrator (FaRAGE) for real-time in situ measurement of dissolved CH4 and CO2 concentrations at the water surface and in the water column. FaRAGE can achieve an exceptionally short response time (t95 %=12 s when including the response time of the gas analyzer) while retaining an equilibration ratio of 62.6 % and a measurement accuracy of 0.5 % for CH4. A similar performance was observed for dissolved CO2 (t95 %=10 s, equilibration ratio 67.1 %). An equilibration ratio as high as 91.8 % can be reached at the cost of a slightly increased response time (16 s). The FaRAGE is capable of continuously measuring dissolved CO2 and CH4 concentrations in the nM-to-sub mM (10−9–10−3 mol L−1) range with a detection limit of sub-nM (10−10 mol L−1), when coupling with a cavity ring-down greenhouse gas analyzer (Picarro GasScouter). FaRAGE allows for the possibility of mapping dissolved concentration in a “quasi” three-dimensional manner in lakes and provides an inexpensive alternative to other commercial gas equilibrators. It is simple to operate and suitable for continuous monitoring with a strong tolerance for suspended particles. While the FaRAGE is developed for inland waters, it can be also applied to ocean waters by tuning the gas–water mixing ratio. The FaRAGE is easily adapted to suit other gas analyzers expanding the range of potential applications, including nitrous oxide and isotopic composition of the gases.

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