biomolecule sensing
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Author(s):  
C.A. Chavarin ◽  
A. A. Wiciak ◽  
E. Hardt ◽  
S. Gruessing ◽  
O. Skibitzki ◽  
...  

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4904
Author(s):  
Chi-Chang Wu ◽  
Min-Rong Wang

In this work, a single-crystalline silicon nanobelt field-effect transistor (SiNB FET) device was developed and applied to pH and biomolecule sensing. The nanobelt was formed using a local oxidation of silicon technique, which is a self-aligned, self-shrinking process that reduces the cost of production. We demonstrated the effect of buffer concentration on the sensitivity and stability of the SiNB FET sensor by varying the buffer concentrations to detect solution pH and alpha fetoprotein (AFP). The SiNB FET sensor was used to detect a solution pH ranging from 6.4 to 7.4; the response current decreased stepwise as the pH value increased. The stability of the sensor was examined through cyclical detection under solutions with different pH; the results were stable and reliable. A buffer solution of varying concentrations was employed to inspect the sensing capability of the SiNB FET sensor device, with the results indicating that the sensitivity of the sensor was negatively dependent on the buffer concentration. For biomolecule sensing, AFP was sensed to test the sensitivity of the SiNB FET sensor. The effectiveness of surface functionalization affected the AFP sensing result, and the current shift was strongly dependent on the buffer concentration. The obtained results demonstrated that buffer concentration plays a crucial role in terms of the sensitivity and stability of the SiNB FET device in chemical and biomolecular sensing.


2021 ◽  
Author(s):  
Jiajie Chen ◽  
Youjun Zeng ◽  
Jie Zhou ◽  
Xueliang Wang ◽  
Ruibiao Miyan ◽  
...  

Abstract The widely used surface-based biomolecule sensing scheme has greatly facilitated the investigation of protein-protein interactions in lab-on-a-chip microfluidic systems. However, in most biosensing schemes, the interactions are driven in a passive way: The overall sensing time and sensitivity are totally dependent on the Brownian diffusion process, which has greatly hindered their efficiency, especially at low concentration level or single-molecule analysis. To break this limitation, we developed an all-optical active method termed optothermophoretic flipping (OTF). The biomolecules were first enriched to aggregation and then pushed to their counterparts for effective contact via a flipped thermophoresis. As a proof-of-concept experiment, we tested its performance via antibody-antigen binding on a surface plasmon resonance imaging (SPRi) platform. We achieved 36.9-fold sensitivity enhancement in this first temporal modulated approach that manipulates biomolecules for interaction enhancement. This method promises to be widely adopted in various biosensing platforms that require ultrasensitivity in colloidal sciences and biochemical studies.


Author(s):  
Yijing Tang ◽  
Dong Zhang ◽  
Yanxian Zhang ◽  
Yonglan Liu ◽  
Lirong Cai ◽  
...  

The past decade has witnessed the growing interest and advances in aggregation-induced emission (AIE) molecules as driven by their unique fluorescence/optical properties in particular sensing applications including biomolecule sensing/detection, environmental/health...


Author(s):  
Thennila Muthukumar ◽  
Elangovan Arumugam ◽  
Sharmila Chandrasekaran ◽  
Chelladurai Karuppiah ◽  
Selvakumar Kodirajan

ACS Nano ◽  
2020 ◽  
Vol 14 (10) ◽  
pp. 12993-13003
Author(s):  
Tilman Schlotter ◽  
Sean Weaver ◽  
Csaba Forró ◽  
Dmitry Momotenko ◽  
János Vörös ◽  
...  

2020 ◽  
Vol 8 (48) ◽  
pp. 11090-11095
Author(s):  
Jia Ge ◽  
Jian-Hua Yu ◽  
Hongfen Yang ◽  
Dan Yang ◽  
Ren Cai

A colorimetric method for facile sensing of acid phosphatase activity based on HSA templated MnO2 nanosheets as an efficient biomimetic oxidase.


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