EFFECTS OF COMBINED INOCULATION OF BAMBARA GROUNDNUT (Vigna subterranean L. Verdc.) WITH GLOMUS MOSSEA AND BRADYHRHIZOBIUM JAPONICUM ON NITROGEN AND PHOSPHOROUS UPTAKE IN SHOOT, PLANT BIOMASS, LEAF CHLOROPHYLL AND MYCORRHIZAL INOCULATION EFFICIENCY (MIE).

The response of Bambara groundnut to co-inoculation with Abuscular mychorrhizal fungi (G. mossea) and Bradhyrhizobium japonicum (strain USDA110) with regard to leaf chlorophyll, percentage shoot nitrogen and phosphorus, nodule weight and plant biomass was studied. Bambara plants were grown under screen house conditions in pots. Plants were inoculated with 1ml of B. japonicum USDA 110 strain (109 cfu /ml), Mychorrizal was applied to the plants 10g, and 20g (90 spores/g) and water was applied at 10ml, 20 ml and 50ml every other day. The obtained results showed that dual inoculation activity was able to improve both nitrogen and phosphorus in plant shoot, MIE, but did not improve biomass and leaf chlorophyll when compared with plants subjected to single inoculation with only G. mossea and only B. japonicum. More Nitrogen and Phosphorus was retained in the shoot of plants co-inoculated with B. japonicum and 20g G. mossea when given 50ml of water and also had higher biomass. Leaf chlorophyll reduced in plants as flowering approached. B. japonicum was able to positively influence and establish symbiosis with G.mossea and synergistically effectively act as “mycorrhiza helper bacteria” (MHB) when both were co-inoculated in Bambara plant.

The role of single inoculation and dubie and triple bacterial interaction Azospirullum brasilanse and VAM Glomuse Mossea and phosphate rock in availability NPK In the Rhizosphere Cucumber ( cucmuise stivease L. )

Use biofirtilizer forming for quantity Bacterial Azospirullum braslieanse and VAM Glomus mossea and four levels of rock phosphate ( 0 , 80, 140 , 200 Kg . ha -1) to study the single effect and double and triple interaction in the availability NPK In the Rhizosphere ( cucmuise stivease L. ) . Although the yield contains few nutrients but is considered one of the most important crops in Iraq ( 36). It is fast growing and needs high fertilization of NPK ( 38). It gave a solo biofertilizer bacterial and fungal and the level of rock phosphate 140 Kg ha -1 on characters NPK Measured in soil , achieving the highest significant increase in the values ​​of treatments 50.43 , 49.52 , 50.70 mg N .kg 1 and 16.02 , 15.51 , 16.26 mg P .kg 1 and 191.54 , 192.26 , 192.32 mg K .kg -1 On the sequence compared with Control. The results of the double interaction in bacterial with fungal , bacterial interaction with phosphate rock, and fungi interaction with phosphate rock at 140 Kg -1 .ha Increase in characters NPK Measured in soil , , As it achieved the highest significant increase in treatments values 51.04 , 52.5 , 51.41 mg N .kg - 1 16.28 , 16.47 , 16.81 mg P .kg - 1 and 192.59 , 192.24 , 193.29 mg K .kg -1 On the sequence in relation to its control. The results of the triple interaction Bacterial, fungal, and phosphate rock was given at the level of phosphate rock 140 Kg. Ha -1 increase in characters NPK Measured in soil , if triple interaction achieves the highest significant increase in the values ​​of treatments 52 . 84 mg N .kg - 1 and 16.92 mg P .kg m - 1 and 193.29 mg K .kg m - 1 On the sequence in relation to its control. The results showed that the triple interaction ( A. braslieanse + Glomeas mossea + Phosphate rock ) at level 140 Kg . Ha -1 Phosphate rock gave the highest significant increase in the values ​​of nitrogen, phosphorus and potassium in the soil of the plastic house . As the interaction between these microorganisms was positive and the impact of living one on the other was a catalyst in the increase of availability NPK .