scholarly journals Comparison of a Whole-Blood Interferon γ Assay With Tuberculin Skin Testing for Detecting Latent Mycobacterium tuberculosis Infection

JAMA ◽  
2001 ◽  
Vol 286 (14) ◽  
pp. 1740 ◽  
Author(s):  
Gerald H. Mazurek
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Whole Blood ◽  
Skin Testing ◽  
Interferon Γ ◽  
Tuberculin Skin Testing

Comparison of a whole blood interferon-γ assay with tuberculin skin testing for the detection of tuberculosis infection in hospitalized children in rural India

2007 ◽  
Vol 54 (3) ◽  
pp. 267-276 ◽  
Author(s):  
Sandeep Dogra ◽  
Pratibha Narang ◽  
Deepak K. Mendiratta ◽  
Pushpa Chaturvedi ◽  
Arthur L. Reingold ◽  
...  
Keyword(s):  
Whole Blood ◽  
Skin Testing ◽  
Interferon Γ ◽  
Tuberculosis Infection ◽  
Rural India ◽  
Tuberculin Skin Testing ◽  
Hospitalized Children

scholarly journals Determining Mycobacterium tuberculosis Infection among BCG-Immunised Ugandan Children by T-SPOT.TB and Tuberculin Skin Testing

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47340 ◽  
Author(s):  
Gyaviira Nkurunungi ◽  
Jimreeves E. Lutangira ◽  
Swaib A. Lule ◽  
Hellen Akurut ◽  
Robert Kizindo ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Skin Testing ◽  
Tuberculosis Infection ◽  
Tuberculin Skin Testing ◽  
Mycobacterium Tuberculosis Infection

scholarly journals Serum Antibody Responses to 10 Mycobacterium tuberculosis Proteins, Purified Protein Derivative, and Old Tuberculin in Natural and Experimental Tuberculosis in Rhesus Monkeys

2011 ◽  
Vol 18 (12) ◽  
pp. 2154-2160 ◽  
Author(s):  
Fangui Min ◽  
Yu Zhang ◽  
Ren Huang ◽  
Wende Li ◽  
Yu'e Wu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rhesus Monkeys ◽  
Skin Testing ◽  
Serum Antibody ◽  
Antibody Responses ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tuberculin Skin Testing ◽  
Purified Protein Derivative ◽  
Diagnostic Potential

ABSTRACTOld tuberculin (OT) and purified protein derivative (PPD) are widely used for tuberculin skin testing (TST) in diagnosis of tuberculosis (TB) but often yield poor specificity and anergy in reaction. Therefore, it is necessary to develop new serological methods as a possible auxiliary diagnostic method for TB. In this study, we characterized the dynamic antibody responses of 10 purified recombinant antigens, PPD, and OT in rhesus monkeys experimentally infected withMycobacterium tuberculosisand analyzed the time to antibody detection, antibody levels, and their association with the infectious doses. The antibodies were detected as early as 4 weeks after infection in response to 5 antigens (CFP10, CFP10-ESAT-6, U1, MPT64, and Ag85b). Antibodies against most of the other antigens were detected between 4 and 12 weeks after infection. The levels of antibodies were dose dependant. We further evaluated the serodiagnostic potential of these antigens by using indirect enzyme-linked immunosorbent assay in 71 TST-positive and 90 TST-negative serum samples from monkeys. For all 12 antigens, the median optical density values of TST-positive monkeys were statistically significantly higher than those of TST-negative monkeys (P< 0.001). Among those antigens, Ag85b and CFP10 showed higher diagnostic potential than others. A combination of results from Ag85b, the 38-kDa antigen (Ag38kDa), and Ag14kDa reaches a sensitivity of 95.77%, indicating that these antigens may be ideal cocktails in TB diagnosis.

Trends in Compliance With the Guidelines for Preventing the Transmission of Mycobacterium tuberculosis Among New Jersey Hospitals, 1989 to 1996

1999 ◽  
Vol 20 (05) ◽  
pp. 337-340 ◽  
Author(s):  
Lilia P. Manangan ◽  
Edgar R. Collazo ◽  
Jerome Tokars ◽  
Sindy Paul ◽  
William R. Jarvis
Keyword(s):  
Mycobacterium Tuberculosis ◽  
New Jersey ◽  
Skin Testing ◽  
Multidrug Resistant ◽  
Tuberculin Skin Testing ◽  
Healthcare Facilities ◽  
Patient Admissions ◽  
Mdr Tb ◽  
Respiratory Devices ◽  
Attending Physicians

AbstractObjective:To determine trends in compliance with the guidelines for preventing the transmission ofMycobacterium tuberculosisin healthcare facilities among New Jersey hospitals from 1989 through 1996.Design:A voluntary questionnaire was sent to all 96 New Jersey hospitals in 1992. The 53 that responded were resurveyed in 1996.Results:Of the 96 hospitals surveyed in 1992, 53 (55%) returned a completed questionnaire; 33 (64%) were community, nonteaching hospitals. In 1991, patients with tuberculosis (TB) were admitted at 38 (72%) of 53 hospitals, and from 1989 through 1991, patients with multidrug-resistant (MDR) TB were admitted at 15 (29%) of 52 hospitals. Twenty-nine (57%) of 51 reported having rooms meeting the Centers for Disease Control and Prevention (CDC) criteria for acid-fast bacilli (AFB) isolation. A nonfltted surgical mask was used as a respiratory protective device by healthcare workers (HCWs) at 28 (55%) of 51 hospitals. Attending physicians were included in tuberculin skin-testing (TST) programs at 5 (11%) of 45 hospitals. In the 1996 resurvey, 48 (94%) of 53 surveyed hospitals returned a completed questionnaire; 34 (81%) of 42 had TB patient admissions, and 4 (9%) of 43 had MDR TB patient admissions in 1996. Forty-five (96%) of 47 reported having rooms that met CDC criteria for AFB isolation. N95 respiratory devices were used by HCWs at 45 (94%) of 48 hospitals. Attending physicians were included in the TST programs at 22 (54%) of 41 hospitals.Conclusion:New Jersey hospitals have made improvements in availability of AFB isolation rooms, use of proper respiratory protective devices, and expansion of TST programs for HCWs from 1989 through 1996.

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