Evaluation of Nanoparticles as Contrast Agent for Photoacoustic Imaging in Living Cells

2010 ◽  
pp. 91-99
Author(s):  
Yvonne Kohl ◽  
Hagen Thielecke ◽  
Wolfgang Bost ◽  
Robert Lemor ◽  
Frank Stracke ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Photoacoustic Imaging ◽  
Living Cells

Nanodiamond-enabled biomedical imaging

Nanomedicine ◽  
2020 ◽  
Vol 15 (16) ◽  
pp. 1599-1616
Author(s):  
Yen-Yiu Liu ◽  
Be-Ming Chang ◽  
Huan-Cheng Chang
Keyword(s):  
Magnetic Resonance ◽  
Contrast Agent ◽  
Biomedical Imaging ◽  
Photoacoustic Imaging ◽  
Living Cells ◽  
In Vivo Studies ◽  
Biological Processes ◽  
New Type ◽  
Fluorescent Nanodiamonds

Biomedical imaging allows in vivo studies of organisms, providing valuable information of biological processes at both cellular and tissue levels. Nanodiamonds have recently emerged as a new type of probe for fluorescence imaging and contrast agent for magnetic resonance and photoacoustic imaging. Composed of sp3-carbon atoms, diamond is chemically inert and inherently biocompatible. Uniquely, its matrix can host a variety of optically and magnetically active defects suited for bioimaging applications. Since the first production of fluorescent nanodiamonds in 2005, a large number of experiments have demonstrated that fluorescent nanodiamonds are useful as photostable markers and nanoscale sensors in living cells and organisms. In this review, we focus our discussion on the recent advancements of nanodiamond-enabled biomedical imaging for preclinical applications.

scholarly journals Polypyrrole–methylene blue nanoparticles as a single multifunctional nanoplatform for near-infrared photo-induced therapy and photoacoustic imaging

RSC Advances ◽  
2017 ◽  
Vol 7 (56) ◽  
pp. 35027-35037 ◽  
Author(s):  
Thi Tuong Vy Phan ◽  
Subramaniyan Bharathiraja ◽  
Van Tu Nguyen ◽  
Madhappan Santha Moorthy ◽  
Panchanathan Manivasagan ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Contrast Agent ◽  
Near Infrared ◽  
Photoacoustic Imaging

A novel contrast agent with broad NIR absorbing properties for combined photo-induced therapy and photoacoustic imaging.

Antimonene Nanoflakes as a Photoacoustic Imaging Contrast Agent for Tumor in vivo Imaging

2020 ◽  
Vol 47 (2) ◽  
pp. 0207033
Author(s):  
于静文 Yu Jingwen ◽  
王秀翃 Wang Xiuhong ◽  
冯金超 Feng Jinchao ◽  
张娜 Zhang Na ◽  
王璞 Wang Pu
Keyword(s):  
Contrast Agent ◽  
In Vivo Imaging ◽  
Photoacoustic Imaging ◽  
Imaging Contrast Agent

Synthesis of a dual contrast agent for ultrasound and photoacoustic imaging

2010 ◽  
Author(s):  
Katheryne Wilson ◽  
Kimberly Homan ◽  
Stanislav Emelianov
Keyword(s):  
Contrast Agent ◽  
Photoacoustic Imaging

scholarly journals Photoacoustic Properties of Polypyrrole Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2457
Author(s):  
Peter Keša ◽  
Monika Paúrová ◽  
Michal Babič ◽  
Tomáš Heizer ◽  
Petr Matouš ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Photoacoustic Imaging ◽  
Particle Surface ◽  
Photoacoustic Signal ◽  
Ammonium Persulphate ◽  
Coated Nanoparticles ◽  
Polypyrrole Nanoparticles ◽  
Signal Dependence

Photoacoustic imaging, an emerging modality, provides supplemental information to ultrasound imaging. We investigated the properties of polypyrrole nanoparticles, which considerably enhance contrast in photoacoustic images, in relation to the synthesis procedure and to their size. We prepared polypyrrole nanoparticles by water-based redox precipitation polymerization in the presence of ammonium persulphate (ratio nPy:nOxi 1:0.5, 1:1, 1:2, 1:3, 1:5) or iron(III) chloride (nPy:nOxi 1:2.3) acting as an oxidant. To stabilize growing nanoparticles, non-ionic polyvinylpyrrolidone was used. The nanoparticles were characterized and tested as a photoacoustic contrast agent in vitro on an imaging platform combining ultrasound and photoacoustic imaging. High photoacoustic signals were obtained with lower ratios of the oxidant (nPy:nAPS ≥ 1:2), which corresponded to higher number of conjugated bonds in the polymer. The increasing portion of oxidized structures probably shifted the absorption spectra towards shorter wavelengths. A strong photoacoustic signal dependence on the nanoparticle size was revealed; the signal linearly increased with particle surface. Coated nanoparticles were also tested in vivo on a mouse model. To conclude, polypyrrole nanoparticles represent a promising contrast agent for photoacoustic imaging. Variations in the preparation result in varying photoacoustic properties related to their structure and allow to optimize the nanoparticles for in vivo imaging.

scholarly journals Photoacoustic molecular imaging using combined acupuncture and gold nanorods as a composite contrast agent

2019 ◽  
Vol 12 (03) ◽  
pp. 1941004
Author(s):  
Dan Wu ◽  
Xinxin Zhang ◽  
Jian Rong ◽  
Huabei Jiang
Keyword(s):  
Contrast Agent ◽  
Contrast Enhancement ◽  
Mouse Brain ◽  
Gold Nanorods ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Local Concentration ◽  
Quantitative Results ◽  
Zusanli Acupoint

In this study, we developed a novel photoacoustic imaging technique based on poly (ethyleneglycol)-coated (PEGylated) gold nanorods (PEG-GNRs) (as the contrast agent) combined with traditional Chinese medicine (TCM) acupuncture (as the auxiliary method) for quantitatively monitoring contrast enhancement in the vasculature of a mouse brain in vivo. This study takes advantage of the strong near-infrared absorption (peak at [Formula: see text][Formula: see text]nm) of GNRs and the ability to adjust the hemodynamics of acupuncture. Experimental results show that photoacoustic tomography (PAT) successfully reveals the optical absorption variation of the vasculature of the mouse brain in response to intravenous administration of GNRs and acupuncture at the Zusanli acupoint (ST36) both individually and combined. The quantitative measurement of contrast enhancement indicates that the composite contrast agents (integration of acupuncture and GNRs) would greatly enhance the photoacoustic imaging contrast. The quantitative results also have the potential to estimate the local concentration of GNRs and even the real-time effects of acupuncture.

Preparation of Lipid Nanovesicle Contrast Material and Its Application in Ultrasound Diagnosis of Breast Cancer Tumor

2020 ◽  
Vol 12 (7) ◽  
pp. 1099-1108
Author(s):  
Siqin Li ◽  
Huicheng Lv
Keyword(s):  
Breast Cancer ◽  
Contrast Agent ◽  
Light Absorption ◽  
Photoacoustic Imaging ◽  
Contrast Material ◽  
Ultrasound Diagnosis ◽  
Strong Light ◽  
Good Imaging ◽  
Significant Difference

In this study, a kind of melanin lipid nanovesicle contrast agent (M-lvca) material with good light absorption and melanin doping is proposed. In this contrast material, lipid nanospheres containing melanin are obtained by ether titration. Then, it is prepared by freeze-drying. In the experimental part, the shape, structure, particle size and absorption spectrum of the material are studied. In vitro agarose gel model, the ultrasound/photoacoustic analysis of the contrast agent is carried out to detect the effect of different concentration of contrast agent on phagocytosis of cells. In the diagnosis of breast cancer, the enhancement boundary, the distribution of contrast agent, the enhancement shape, the range change after enhancement, and the penetration of blood vessels are detected. The experimental results show that the contrast agent has the characteristics of microsphere shape, uniform distribution and strong light absorption ability in the wavelength range of 100 nm∼900 nm. In vitro ultrasound imaging/photoacoustic imaging test, the contrast agent has a good imaging ability. With the increase of the concentration, the imaging ability will be further enhanced. In the diagnosis of breast cancer, there is a significant difference in the indexes related to ultrasound diagnosis of breast cancer after adding M-lvca contrast agent (P < 0.05). Only a few indexes such as enhancement intensity and perfusion defect have no significant difference (P > 0.05). In this study, the proposed M-lvca material can improve the imaging effect of related indicators in the early diagnosis of breast cancer, which is helpful to improve the accuracy of breast cancer diagnosis.

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