Kiwifruit and Human Platelet Function

Effects of inhaled nitric oxide (NO) on human platelet function are controversial. It is uncertain whether intraplatelet cGMP mediates the effect of inhaled NO on platelet function. We investigated the effect of 30 ppm inhaled NO on platelet aggregation and plasma and intraplatelet cGMP in 12 subjects. We performed platelet aggregation studies by using a photooptical aggregometer and five agonists (ADP, collagen, epinephrine, arachidonic acid, and ristocetin). During inhalation, the maximal extent of platelet aggregation decreased by 75% with epinephrine ( P < 0.005), 56% with collagen ( P < 0.005), and 20% with arachidonic acid ( P < 0.05). Responses to ADP (8% P > 0.05) and ristocetin (5% P > 0.05) were unaffected. Platelet aggregation velocity decreased by 64% with collagen ( P < 0.005), 60% with epinephrine ( P < 0.05), 33% with arachidonic acid ( P < 0.05), and 14% with ADP ( P > 0.05). Plasma cGMP levels increased from 2.58 ± 0.43 to 9.99 ± 5.57 pmol/ml ( P < 0.005), intraplatelet cGMP levels were unchanged (means ± SD: 1.96 ± 0.58 vs. 2.71 ± 1.67 pmol/109platelets; P > 0.05). Inhaled NO inhibits platelet aggregation via a cGMP independent mechanism.

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Human platelet microRNA-mRNA networks associated with age and gender revealed by integrated plateletomics

Blood ◽

10.1182/blood-2013-12-544692 ◽

2014 ◽

Vol 123 (16) ◽

pp. e37-e45 ◽

Cited By ~ 123

Author(s):

Lukas M. Simon ◽

Leonard C. Edelstein ◽

Srikanth Nagalla ◽

Angela B. Woodley ◽

Edward S. Chen ◽

...

Keyword(s):

Gene Expression ◽

Platelet Function ◽

Human Platelet ◽

Web Tool ◽

Age And Gender ◽

Key Points ◽

And Gender ◽

Unique Dataset

Key Points Unique dataset of human platelet mRNA, miRNA, and physiology reveals mRNAs and miRNAs that differ by age and gender. Interactive public web tool (www.plateletomics.com) provides biologic insights into platelet function and gene expression.

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Preliminary Comparison of the Effects of Iloprost, Prostacyclin (PGI2) and Prostaglandin E1 (PGE1) on Human Platelet Function

Prostacyclin and Its Stable Analogue Iloprost ◽

10.1007/978-3-642-71499-3_8 ◽

1987 ◽

pp. 65-68 ◽

Cited By ~ 2

Author(s):

C. A. Fisher ◽

H. J. Reiser ◽

V. P. Addonizio

Keyword(s):

Platelet Function ◽

Prostaglandin E1 ◽

Human Platelet ◽

Preliminary Comparison

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Lack Of Inhibition Of Thromboxane Production Despite Inhibition Of Platelet Function By 1,3-Bis(2 Chloroethyl)-1-Nitrosourea (BCNU)

10.1055/s-0038-1652605 ◽

1981 ◽

Author(s):

R McKenna ◽

T Ahmad ◽

A Prancan ◽

D Simon ◽

H Frischer

Keyword(s):

Arachidonic Acid ◽

Oxygen Consumption ◽

Platelet Aggregation ◽

Acetylsalicylic Acid ◽

Platelet Function ◽

Human Platelet ◽

Thromboxane A2 ◽

Rabbit Aorta ◽

Thromboxane Production ◽

Platelet Thromboxane

We have previously shown that BCNU inhibits human platelet glutathione reductase (GSSG-R) prior to inhibiting platelet function; since thromboxane production is important in platelet function, we evaluated the effect of BCNU induced inhibition of GSSG-R on platelet thromboxane production.Control platelet GSSG-R activity was 0.091 ]jmoles NAD(P)H oxidized min-1lmg-1 protein at 37°C (±0.015 S.D.; n=9); inhibition was detectable at 10-7M% BCNU (70% of control) with a >90% inhibition at and above 10-5M BCNU. Platelet aggregation in response to 1.5×10-3M Arachidonic acid (AA), 10 μM epinephrine, 6 μg/ml equine collagen and 3 μM ADP were inhibited at 10-5M BCNU and abolished at 10-4 BCNU.BCNU (10-3M) did not affect the increase in oxygen consumption induced by AA. Using the rabbit aorta superfusion bioassay for thromboxane A2 (TXA2), threshold concentrations of AA in 10-5 and 10-4 BCNU platelets resulted in an increased measure of aortic tension 13.5 ± 9.4 mm S.D. (n=6) and 23.2 ± 9.5 mm respectively, compared with control values of 4.5 ± 2.4. Acetylsalicylic acid (5 × l0-4M) inhibited the contraction: 1.7 ± 1.1 (n=5). The conversion of 14C AA to thromboxane B2 (TXB2) and PGE2, as measured by radio TLC, was not decreased in BCNU treated platelets. There is a significant increase in TXB2 (p<0.05;n=4) and in the ratio of TXB2:PGE2 in platelets treated with 10-4M BCNU and 10-3M imidazole when compared to platelets treated with imidazole alone.In conclusion BCNU induced inhibition of platelet GSSG-R and platelet function occurs despite preservation of thromboxane production

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