Oxytocin and Epstein-Barr virus: Stress biomarkers in the postpartum period among first-time mothers from São Paulo, Brazil

ABSTRACT BZLF1 plays a key role in the induction of Epstein-Barr virus (EBV) replication. On the basis of limited sequence homology and mutagenesis experiments, BZLF1 has been described as a member of the bZip family of transcription factors, but this prospect has not been rigorously tested to date. Here, we present biophysical analysis of the multimerization domain of BZLF1, from three natural variants of EBV, and demonstrate for the first time that the region between amino acids 196 and 227 is sufficient to direct folding as a coiled-coil dimer in vitro.

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THE INFLUENCE OF POINT MUTATIONS IN THE EPSTEIN-BARR VIRUS LMP1 ONCOGENE ON THE CELL CYTOSKELETON AND ACTIVATION OF INDUCIBLE FORM OF NO SYNTHASE

Annals of the Russian academy of medical sciences ◽

10.15690/vramn.v67i3.187 ◽

2012 ◽

Vol 67 (3) ◽

pp. 62-67 ◽

Cited By ~ 1

Author(s):

S. V. Diduk ◽

K. V. Smirnova ◽

V. E. Gurtsevitch

Keyword(s):

Epstein Barr Virus ◽

Point Mutations ◽

No Synthase ◽

Latent Membrane Protein 1 ◽

Structural Components ◽

Barr Virus ◽

Individual Point ◽

Epstein Barr ◽

Oxide Synthase ◽

First Time

One of the latent proteins encoded by the Epstein−Barr virus (EBV), the latent membrane protein 1 (LMP1), plays a key role in developing of EBV-associated human malignancies. Polymorphism of LMP1 protein is its characteristic feature. Some specific mutations in LMP1 genome have previously been detected in different geographic regions, however, the influence of these mutations on functional activity of LMP1 was not still determined. In this study we demonstrated for the first time the significance of individual point mutations among common ones observed in LMP1 and their combination on activation of the inducible form of nitric oxide synthase (iNOS). In addition, the influence of above mutations localized in the CTAR regions of the LMP1 molecule has also been investigated on structural components of the fibroblasts of the Rat1cell line.

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Differential Hyperacetylation of Histones H3 and H4 upon Promoter-Specific Recruitment of EBNA2 in Epstein-Barr Virus Chromatin

Journal of Virology ◽

10.1128/jvi.77.14.8166-8172.2003 ◽

2003 ◽

Vol 77 (14) ◽

pp. 8166-8172 ◽

Cited By ~ 37

Author(s):

Nathalie Alazard ◽

Henri Gruffat ◽

Edwige Hiriart ◽

Alain Sergeant ◽

Evelyne Manet

Keyword(s):

B Lymphocytes ◽

Chromatin Immunoprecipitation ◽

Epstein Barr Virus ◽

Nuclear Antigen ◽

Barr Virus ◽

Viral Promoters ◽

Epstein Barr ◽

Cellular Dna ◽

First Time

ABSTRACT Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transcriptional activator involved in the immortalization of B lymphocytes by the virus. EBNA2 is targeted to the promoters of its responsive genes, via interaction with cellular DNA-binding proteins. Using chromatin immunoprecipitation assays, we show for the first time the conditional recruitment of EBNA2 on two specific viral promoters in vivo and demonstrate a correlation between this recruitment and a local change in the acetylation of histones H3 and H4, which is promoter dependent.

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C-Terminal Farnesylation of UCH-L1 Plays a Role in Transport of Epstein-Barr Virus Primary Oncoprotein LMP1 to Exosomes

mSphere ◽

10.1128/msphere.00030-18 ◽

2018 ◽

Vol 3 (1) ◽

Cited By ~ 12

Author(s):

E. Kobayashi ◽

M. Aga ◽

S. Kondo ◽

C. Whitehurst ◽

T. Yoshizaki ◽

...

Keyword(s):

Cancer Progression ◽

Epstein Barr Virus ◽

Cancer Metastasis ◽

Cell Communication ◽

B Cell Lymphoma ◽

Latent Membrane Protein 1 ◽

Potential Therapeutic Target ◽

Barr Virus ◽

Epstein Barr ◽

First Time

Exosomes are small vesicles that cells secrete into the extracellular space, and there is increasing evidence that they have pivotal roles in cell-to-cell communication in malignancy. It is reported also that EBV-associated malignant cells, including those derived from nasopharyngeal carcinoma (NPC) and B-cell lymphoma, secrete exosomes. These EBV-related exosomes may contain viral products such as latent membrane protein 1 (LMP1) and may contribute to cancer progression. The aim of this study was to investigate the mechanism by which those viral products are loaded in exosomes. In this study, we show for the first time that ubiquitin C-terminal hydrolase-L1 (UCH-L1) and its C-terminal farnesylation, a posttranslational lipid modification, contribute to this mechanism. Our results also suggest that inhibition of UCH-L1 farnesylation is a potential therapeutic target against cancer metastasis and invasion.

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Lack of association between Epstein–Barr virus and mammary tumours in dogs

Journal of Veterinary Research ◽

10.2478/jvetres-2018-0045 ◽

2018 ◽

Vol 62 (3) ◽

pp. 309-315

Author(s):

Gustavo A. Roa López ◽

Jhon Jairo Suárez ◽

Paola Barato ◽

Noel Verján García

Keyword(s):

Breast Cancer ◽

Mammary Gland ◽

Epstein Barr Virus ◽

Barr Virus ◽

Mammary Tumours ◽

Mammary Gland Cancer ◽

Epstein Barr ◽

And Control ◽

First Time

AbstractIntroductionEpstein–Barr virus (EBV) is a γ-herpesvirus associated with various neoplasms in humans and is a probable aetiological agent in breast cancer; however, a causal relationship has not yet been established. Because of the epidemiological and clinicopathological similarities between breast cancer and canine mammary tumours, dogs have been proposed as a valid model for breast cancer.Material and MethodsA total of 47 canine mammary gland tumour tissues were processed by routine histopathological technique with haematoxylin-eosin staining and classified according to the type of neoplasm. DNA was extracted from paraffin-embedded tissues and the EBNA-1 gene and the BamHI-W region specific for EBV were evaluated by nested PCR.ResultsThe histopathological evaluation revealed 2 benign neoplasms, and many carcinomas: 2 in situ, 9 simple, 3 solid, 10 complex, and 21 mixed. One sample was positive for the EBNA-1 gene, while all were negative for the BamHI-W region.ConclusionNo association was found between EBV and mammary tumours in dogs. However, here we report for the first time the presence of an EBV gene sequence in a canine mammary tumour. It is likely that detection of EBV might be affected by the quality and quantity of DNA extracted from paraffin-embedded tissues. Additional studies are necessary to establish any association of EBV with mammary gland cancer in humans and in dogs, which could eventually lead to better public health prevention and control.

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SCREENING OF HEPATITIS G AND EPSTEIN-BARR VIRUSES AMONG VOLUNTARY NON REMUNERATED BLOOD DONORS (VNRBD) IN BURKINA FASO, WEST AFRICA

Mediterranean Journal of Hematology and Infectious Diseases ◽

10.4084/mjhid.2013.053 ◽

2013 ◽

Vol 5 (1) ◽

pp. e2013053 ◽

Cited By ~ 4

Author(s):

Issouf Tao ◽

Cyrille Bisseye ◽

Bolni Marius Nagalo ◽

Mahamoudou Sanou ◽

Alice Kiba ◽

...

Keyword(s):

Hepatitis B ◽

Burkina Faso ◽

Epstein Barr Virus ◽

Blood Donors ◽

Barr Virus ◽

Hepatitis G ◽

Epstein Barr ◽

Sub Saharan ◽

Hepatitis B Antigens ◽

First Time

In most sub-Saharan countries screening of blood-transmitted infections includes mainly HIV, HBV, HCV and syphilis. Many viruses such as Hepatitis G (HGV) and Epstein-Barr virus (EBV) which also carry a risk of transmission by blood transfusion raise the question of the extent of screening for these pathogens. This work aims to evaluate the prevalence of HGV and EBV in first-time blood donors in Ouagadougou. The prevalence of HGV and EBV in 551 blood donors was 7.4% and 5.4% respectively. HGV prevalence was significantly higher in blood donors with hepatitis B antigens and positive for HCV compared to donors negative for HCV and no hepatitis B antigens (respectively p<0.001 and p=0.004). EBV prevalence was higher among blood donors of < 20 years age group. This study shows significant results with regard to the prevalence of HGV and EBV prevalence in blood donors in Burkina Faso and emphasizes the need for a general screening.

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Regulation of Matrix Metalloproteinase 9 Expression by Epstein-Barr Virus Nuclear Antigen 3C and the Suppressor of Metastasis Nm23-H1

Journal of Virology ◽

10.1128/jvi.79.15.9714-9724.2005 ◽

2005 ◽

Vol 79 (15) ◽

pp. 9714-9724 ◽

Cited By ~ 22

Author(s):

Daniel A. Kuppers ◽

Ke Lan ◽

Jason S. Knight ◽

Erle S. Robertson

Keyword(s):

Transcription Factors ◽

Binding Sites ◽

Epstein Barr Virus ◽

Nuclear Antigen ◽

Barr Virus ◽

Guanine Exchange Factor ◽

Epstein Barr ◽

First Time ◽

Metalloproteinase 9

ABSTRACT Epstein-Barr virus latent protein EBNA3C has been shown to bind Nm23-H1, a known suppresser of cell migration and metastasis and a regulator of the guanine exchange factor Tiam-1. This interaction results in cellular translocation of Nm23-H1 to the nucleus and suppression of the antimigratory effect in vitro. Furthermore, these proteins can synergistically increase transcription of a basal promoter when targeted to DNA by fusion to a Gal4 DNA binding domain. In this report, we show that EBNA3C and Nm23-H1 can cooperate to upregulate expression of MMP-9, known to be expressed in aggressive forms of lymphomas. This upregulation resulted in increased levels of MMP-9 mRNA, as well as a detectable increase in MMP-9 gelatinolytic activity. Specific mutations in the MMP-9 promoter showed that the Ap1 and NFκB binding sites are important for upregulation by the proteins. Additionally, it was shown for the first time that EBNA3C and Nm23-H1 can bind subunits of these transcription factors. This suggests that the ability of EBNA3C to reverse the antimigratory effects of Nm23-H1 is likely to be in part through the synergistic upregulation of MMP-9, mediated through interactions with the AP1 and NFκB transcription factors.

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