Amplified fluorescence target DNA detection was developed combining nicking endonuclease assisted target recycling and magnetic nanoparticles with low background signal.
This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in thein situfluorescence signal amplification.
Expansion microscopy was applied to the observation of primary cilia and centrioles. To compensate for the decrease in fluorescence signal per unit volume inherent in physical expansion, a fluorescence signal amplification tool, called the amplibody, was developed. The method enables the practical observation of cilia and centrioles with high brightness and resolution.
A new two-mode fluorescence signal amplification strategy for protease activity assay based on graphene oxide
