scholarly journals Nanoformulated Single‐Stranded RNA‐Based Adjuvant with a Coordinative Amphiphile as an Effective Stabilizer: Inducing Humoral Immune Response by Activation of Antigen‐Presenting Cells

2020 ◽  
Vol 132 (28) ◽  
pp. 11637-11646
Author(s):  
Hyo‐Jung Park ◽  
Eun‐Kyoung Bang ◽  
Jung Joo Hong ◽  
Sang‐Myeong Lee ◽  
Hae Li Ko ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Antigen Presenting Cells ◽  
Humoral Immune ◽  
Effective Stabilizer ◽  
Antigen Presenting

scholarly journals Humoral immune response in mice against a circulating antigen induced by adenoviral transfer is strictly dependent on expression in antigen-presenting cells

Blood ◽  
2003 ◽  
Vol 101 (7) ◽  
pp. 2551-2556 ◽  
Author(s):  
Bart R. De Geest ◽  
Sophie A. Van Linthout ◽  
Désiré Collen
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Time Course ◽  
Antigen Presenting Cells ◽  
Antigen Expression ◽  
Critical Determinant ◽  
Humoral Immune ◽  
Circulating Antigen ◽  
Adenoviral Transfer ◽  
Antigen Presenting

Adenoviral transfer of human apo A-I in Balb/c mice induces a strong humoral immune response against the transgene product when expression is driven from the ubiquitously active CMVpromoter but induces no immune response when driven by the hepatocyte-specific 256–base pair apo A-I promoter. Here the hypothesis was tested, which is that the humoral immune response against the circulating transgene product correlates with its expression in antigen-presenting cells. No humoral immune response was observed after adenoviral transfer of vectors with human apo A-I expression driven by the hepatocyte-specific apo C-II or 1.5-kilobase (kb) humanα1-antitrypsin promoter, but antibodies were induced after transfer with vectors driven by the ubiquitously activeU1b promoter and the murine MHCII Eβpromoter. A strict correlation was observed between antigen expression in the spleen and the occurrence of an immune response. Coinjection of the 1.5-kb human α1-antitrypsin and the murine MHCII Eβ promoter–driven vectors resulted in a very short-lived humoral immune response against human apo A-I, suggesting that the time course of human apo A-I expression is a critical determinant of the development of tolerance for human apo A-I. High titers of antibodies against human apo A-I after subcutaneous gene transfer with the MHCII Eβ promoter–driven vector underscore the potential of this promoter for vaccination purposes. In conclusion, humoral immune response in mice against a circulating antigen induced by adenoviral transfer is strictly dependent on expression in antigen-presenting cells.

Role of Yersinia pseudotuberculosis outer proteins (Yops) in murine humoral immune response

2009 ◽  
Vol 54 (3) ◽  
pp. 239-245 ◽  
Author(s):  
J. M. L. Maia ◽  
L. G. S. Monnazzi ◽  
B. M. M. Medeiros
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Yersinia Pseudotuberculosis ◽  
Humoral Immune

scholarly journals Diagnostic and analytical performance evaluation of ten commercial assays for detecting SARS-CoV-2 humoral immune response

2021 ◽  
pp. 113043
Author(s):  
Marnix Mylemans ◽  
Eveline Van Honacker ◽  
Louis Nevejan ◽  
Stefanie van den Bremt ◽  
Laura Hofman ◽  
...  
Keyword(s):  
Immune Response ◽  
Performance Evaluation ◽  
Humoral Immune Response ◽  
Analytical Performance ◽  
Humoral Immune

scholarly journals SARS-CoV-2 RNA and antibodies in tear fluid

2021 ◽  
Vol 6 (1) ◽  
pp. e000733
Author(s):  
Astrid Muyldermans ◽  
Maria Bjerke ◽  
Thomas Demuyser ◽  
Deborah De Geyter ◽  
Ingrid Wybo ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Spike Protein ◽  
Tear Fluid ◽  
Local Response ◽  
Schirmer Test ◽  
Humoral Immune ◽  
Non Invasive ◽  
Reverse Transcription Pcr ◽  
Ocular Symptoms

Background/aimsSARS-CoV-2 is highly contagious. More evidence concerning extrapulmonary transmission routes such as the eyes is urgently needed. Although the humoral immune response is important in the viral containment, the local response in tears has not yet been studied. The aim of our study was twofold: to assess the prevalence of both SARS-CoV-2 RNA and antibodies in tear fluid.MethodsIn a first series, nasopharyngeal sampling and tear sampling by Schirmer test strips were performed in 26 acutely ill patients with COVID-19 to assess the presence of SARS-CoV-2 RNA by reverse transcription PCR. In a second series, IgG and IgA responses to SARS-CoV-2 spike protein in serum and tear fluid of convalescent individuals (n=22) were compared with control individuals (n=15) by ELISA.ResultsSARS-CoV-2 RNA was detected in tears of 7/26 (26.9%) patients with COVID-19. None of them had ocular symptoms. Convalescent individuals displayed a significant higher ratio of IgG (p<0.0001) and IgA (p=0.0068) in tears compared with control individuals. A sensitivity of 77.3% and specificity of 93.3% was observed for IgG, and 59.1% and 100% for IgA.ConclusionsOur results demonstrate the presence of SARS-CoV-2 RNA and a local IgG and IgA immune response in tear fluid. These data confirm the possibility of SARS-CoV-2 transmission through tear fluid and the importance of the eye as a first defence against SARS-CoV-2, indicating the potential of tears as a non-invasive surrogate for serum in monitoring the host immune response.

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