scholarly journals Retardation of colony growth of in vitro bone marrow culture using sera from patients with felty' syndrome, disseminated lupus erythematosus (sle), rheumatoid arthritis, and other disease states

1975 ◽  
Vol 18 (4) ◽  
pp. 323-333 ◽  
Author(s):  
Dewey J. Duckham ◽  
Robert L. Rhyne ◽  
Fredrica E. Smith ◽  
Ralph C. Williams
Keyword(s):  
Rheumatoid Arthritis ◽  
Bone Marrow ◽  
Lupus Erythematosus ◽  
Colony Growth ◽  
Bone Marrow Culture ◽  
Disease States ◽  
Felty Syndrome

scholarly journals Brief Report: Stimulation of Bone Marrow Colony Growth In Vitro by Human Urine

Blood ◽  
1969 ◽  
Vol 33 (3) ◽  
pp. 396-399 ◽  
Author(s):  
W. A. ROBINSON ◽  
E. R. STANLEY ◽  
D. METCALF
Keyword(s):  
Bone Marrow ◽  
Cell Growth ◽  
Mononuclear Cell ◽  
Human Urine ◽  
Colony Growth ◽  
Urine Samples ◽  
Disease States ◽  
Normal Humans ◽  
Stimulation Of

Abstract Using a new technic of bone marrow culture in agar, urine samples from 50 humans have been tested for their ability to stimulate the formation of granulocyte—mononuclear cell growth in vitro. Significant colony stimulating activity has been found with 25 out of 50 unconcentrated urine samples from both normal humans and patients with a variety of disease states.

scholarly journals Cell-mediated amegakaryocytic thrombocytopenia associated with systemic lupus erythematosus

Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 479-483
Author(s):  
T Nagasawa ◽  
T Sakurai ◽  
H Kashiwagi ◽  
T Abe
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Bone Marrow ◽  
T Cells ◽  
Lupus Erythematosus ◽  
Culture System ◽  
Bone Marrow Cells ◽  
Rare Complication ◽  
Systemic Lupus ◽  
Marrow Cells

We studied a patient with a rare complication of amegakaryocytic thrombocytopenia (AMT) associated with systemic lupus erythematosus (SLE). To investigate the underlying pathogenesis of AMT, the effects of peripheral blood T cells and serum on human megakaryocyte progenitor cells were studied using in vitro coculture techniques. Mononuclear bone marrow cells (2 X 10(5) from normal donors produced 33.6 +/- 8.8 (n = 10) colony-forming unit-megakaryocytes (CFU-M) in our plasma clot system. When 2 X 10(5) of the patient's T cells were added to the culture system, the number of CFU-M decreased to only 3.5 +/- 0.6/2 X 10(5) bone marrow cells. No evidence of inhibitory effects was found by the addition of the patient's serum and complement to the culture system. The T cells stored at -80 degrees C on admission were also capable of suppressing autologous CFU-M after recovery from AMT. These results indicate that in vitro suppression of CFU-M from allogenic and autologous bone marrow cells by this patient's T cells provides an explanation for the pathogenesis of AMT associated with SLE.

Prognostic Value of in Vitro Bone Marrow Culture in Refractory Anaemia with Excess of Myeloblasts

2009 ◽  
Vol 20 (3) ◽  
pp. 280-286 ◽  
Author(s):  
A. Faille ◽  
C. Dresch ◽  
O. Poirier ◽  
N. Balitrand ◽  
Y. Najean
Keyword(s):  
Bone Marrow ◽  
Prognostic Value ◽  
Bone Marrow Culture ◽  
Refractory Anaemia

scholarly journals Cytogenetic studies and in vitro colony growth in patients with mastocytosis

Blood ◽  
1987 ◽  
Vol 70 (6) ◽  
pp. 1928-1932 ◽  
Author(s):  
B Swolin ◽  
S Rodjer ◽  
G Roupe
Keyword(s):  
Bone Marrow ◽  
Growth Pattern ◽  
Bone Marrow Cells ◽  
Systemic Mastocytosis ◽  
Colony Growth ◽  
Chromosome Abnormalities ◽  
Control Group ◽  
Normal Peripheral Blood ◽  
Abnormal Growth

Abstract Cytogenetic analysis of bone marrow cells and in vitro growth for bone marrow granulocytic-macrophage stem cells have been performed in 13 patients with mastocytosis, six with systemic mastocytosis, and seven with urticaria pigmentosa. Clones with chromosome abnormalities were found in five patients. The number of clusters and/or colonies after seven days in culture was increased in seven patients, compared with the growth in a control group. Three patients with chromosome abnormalities showed an abnormal growth pattern, yet exhibited normal peripheral blood values. Two patients with systemic mastocytosis had clones with chromosome abnormalities and some abnormal hematological values. The proportion of patients with chromosome abnormalities and an abnormal growth pattern was higher among these patients with mastocytosis than in healthy control subjects. These results may be of interest when discussing the origin of mast cell disorders and indicate an association with the myeloproliferative disorders.

scholarly journals Methyldopa: Physicochemical Characterization of the Erythrocyte Autoantibody

Blood ◽  
1973 ◽  
Vol 42 (2) ◽  
pp. 247-255 ◽  
Author(s):  
Barry Wenz ◽  
Parviz Lalezari
Keyword(s):  
Temperature Gradient ◽  
Lupus Erythematosus ◽  
Physicochemical Characterization ◽  
Red Cell ◽  
Disease States ◽  
Graphic Data ◽  
Cell Antibody ◽  
Dissociation Curves ◽  
Antigen Antibody

Abstract A modification of the Polybrene technique for red blood cell antibody characterization has been employed to differentiate the panhemagglutinins arising during methyldopa administration from those accompanying other disease states. Dissociation characteristics of methyldopa-associated antigen-antibody complexes were determined by temperature gradient dissociation technique. Data obtained by this technique for cell-bound antibody were found to distinguish this antibody from those accompanying systemic lupus erythematosus (SLE) and Pronestyl therapy. Graphic data derived from temperature gradient dissociation curves at varying antibody concentrations were obtained for the methyldopa-induced serum antibodies. Results obtained with samples from all six patients were found to be relatively uniform in relation to each other, and different from similarly derived results for red cell antibodies accompanying idiopathic autoimmune hemolytic anemia and Hodgkin’s disease. By means of these procedures, as well as standard blood banking techniques, distinguishing features are described that permit in vitro segregation of these distinct groups of red cell autoantibodies.

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