Biotransformation and chiral resolution of d , l ‐alanine into pyruvate and d ‐alanine with a whole‐cell biocatalyst expressing l ‐amino acid deaminase

2020 ◽  
Vol 67 (4) ◽  
pp. 668-676 ◽  
Author(s):  
Ke Liu ◽  
Mengyue Gong ◽  
Xueqin Lv ◽  
Jianghua Li ◽  
Guocheng Du ◽  
...  
Keyword(s):  
Amino Acid ◽  
Chiral Resolution ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

Bioconversion of l-glutamic acid to α-ketoglutaric acid by an immobilized whole-cell biocatalyst expressing l-amino acid deaminase from Proteus mirabilis

2014 ◽  
Vol 169 ◽  
pp. 112-120 ◽  
Author(s):  
Gazi Sakir Hossain ◽  
Jianghua Li ◽  
Hyun-dong Shin ◽  
Rachel R. Chen ◽  
Guocheng Du ◽  
...  
Keyword(s):  
Amino Acid ◽  
Glutamic Acid ◽  
Proteus Mirabilis ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

Transporter engineering and enzyme evolution for pyruvate production from d/l-alanine with a whole-cell biocatalyst expressing l-amino acid deaminase from Proteus mirabilis

RSC Advances ◽  
2016 ◽  
Vol 6 (86) ◽  
pp. 82676-82684 ◽  
Author(s):  
Gazi Sakir Hossain ◽  
Hyun-dong Shin ◽  
Jianghua Li ◽  
Guocheng Du ◽  
Jian Chen ◽  
...  
Keyword(s):  
Amino Acid ◽  
Proteus Mirabilis ◽  
Production Method ◽  
Enzyme Evolution ◽  
Whole Cell ◽  
E Coli ◽  
Whole Cell Biocatalyst ◽  
One Step

Pyruvate, which has been widely used in the food, pharmaceutical, and agrochemical industries, can be produced by “one-step pyruvate production” method from d/l-alanine with a whole-cell E. coli biocatalyst expressing l-amino acid deaminase (pm1) from Proteus mirabilis.

scholarly journals Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinghui Xiong ◽  
Hefeng Chen ◽  
Ran Liu ◽  
Hao Yu ◽  
Min Zhuo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Regeneration System ◽  
Nadph Regeneration ◽  
Whole Cell ◽  
Cyclohexanone Monooxygenase ◽  
Ribosome Binding ◽  
Ribosome Binding Sites ◽  
Whole Cell Biocatalyst ◽  
Substrate Tolerance

Abstractε-Caprolactone is a monomer of poly(ε-caprolactone) which has been widely used in tissue engineering due to its biodegradability and biocompatibility. To meet the massive demand for this monomer, an efficient whole-cell biocatalytic approach was constructed to boost the ε-caprolactone production using cyclohexanol as substrate. Combining an alcohol dehydrogenase (ADH) with a cyclohexanone monooxygenase (CHMO) in Escherichia coli, a self-sufficient NADPH-cofactor regeneration system was obtained. Furthermore, some improved variants with the better substrate tolerance and higher catalytic ability to ε-caprolactone production were designed by regulating the ribosome binding sites. The best mutant strain exhibited an ε-caprolactone yield of 0.80 mol/mol using 60 mM cyclohexanol as substrate, while the starting strain only got a conversion of 0.38 mol/mol when 20 mM cyclohexanol was supplemented. The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0.78 mol/mol.

Antityrosinase Mechanism and Antimelanogenic Effect of Arbutin Esters Synthesis Catalyzed by Whole-Cell Biocatalyst

2021 ◽  
Vol 69 (14) ◽  
pp. 4243-4252
Author(s):  
Haixia Xu ◽  
Xiaofeng Li ◽  
Xuan Xin ◽  
Lan Mo ◽  
Yucong Zou ◽  
...  
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds

2014 ◽  
Vol 32 (3) ◽  
pp. 652-662 ◽  
Author(s):  
Chang Sup Kim ◽  
Jeong Hyun Seo ◽  
Dong Gyun Kang ◽  
Hyung Joon Cha
Keyword(s):  
Whole Cell ◽  
Organophosphate Compounds ◽  
Whole Cell Biocatalyst
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