Improved production of α-ketoglutaric acid (α-KG) by a Bacillus subtilis whole-cell biocatalyst via engineering of l-amino acid deaminase and deletion of the α-KG utilization pathway

2014 ◽  
Vol 187 ◽  
pp. 71-77 ◽  
Author(s):  
Gazi Sakir Hossain ◽  
Jianghua Li ◽  
Hyun-dong Shin ◽  
Long Liu ◽  
Miao Wang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Amino Acid ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Utilization Pathway

Bioconversion of l-glutamic acid to α-ketoglutaric acid by an immobilized whole-cell biocatalyst expressing l-amino acid deaminase from Proteus mirabilis

2014 ◽  
Vol 169 ◽  
pp. 112-120 ◽  
Author(s):  
Gazi Sakir Hossain ◽  
Jianghua Li ◽  
Hyun-dong Shin ◽  
Rachel R. Chen ◽  
Guocheng Du ◽  
...  
Keyword(s):  
Amino Acid ◽  
Glutamic Acid ◽  
Proteus Mirabilis ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

Investigation of spore coat display of Bacillus subtilis β-galactosidase for developing of whole cell biocatalyst

2013 ◽  
Vol 195 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Setareh Tavassoli ◽  
Krzysztof Hinc ◽  
Adam Iwanicki ◽  
Michal Obuchowski ◽  
Gholamreza Ahmadian
Keyword(s):  
Bacillus Subtilis ◽  
Spore Coat ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

Transporter engineering and enzyme evolution for pyruvate production from d/l-alanine with a whole-cell biocatalyst expressing l-amino acid deaminase from Proteus mirabilis

RSC Advances ◽  
2016 ◽  
Vol 6 (86) ◽  
pp. 82676-82684 ◽  
Author(s):  
Gazi Sakir Hossain ◽  
Hyun-dong Shin ◽  
Jianghua Li ◽  
Guocheng Du ◽  
Jian Chen ◽  
...  
Keyword(s):  
Amino Acid ◽  
Proteus Mirabilis ◽  
Production Method ◽  
Enzyme Evolution ◽  
Whole Cell ◽  
E Coli ◽  
Whole Cell Biocatalyst ◽  
One Step

Pyruvate, which has been widely used in the food, pharmaceutical, and agrochemical industries, can be produced by “one-step pyruvate production” method from d/l-alanine with a whole-cell E. coli biocatalyst expressing l-amino acid deaminase (pm1) from Proteus mirabilis.

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