Abstract
Background and Objective: To observe the effect of Xijiao Dihuang Decoction (XJDHT) on lung injury of sepsis mice with lipidomic analysis, and explore the underlying mechanisms.Materials and Methods: C57BL/6 mice were induced sepsis by lipopolysaccharide (LPS) administration. Animals are pretreated with XJDHT 2 days before LPS regimen, then continue treatment for addition 3 days. Lung tissue are collected for lipidomic analysis by LC/MS/MS. The survival rate is monitor after treatment. Results: After treatment with XJDHT, the histological changes of lung of the sepsis mice were relieved. The lipidomic profiles of the lung in sepsis group were evidently disordered when compared to the control group. A total of 40 significantly differential lipids expression between the sepsis-control groups were identified by multivariate statistical analysis. Septic lung injury may result from such lipid disorders, which are related to fatty acyls metabolism, glycerophospholipid metabolism, phosphosphingolipids metabolism and glycerides metabolism. After XJDHT treatment, the lipidomic profiles of the disorders tend towards alleviated when compared to the sepsis group. Twelve lipid molecules were identified and some glycerophospholipids levels returned close to the normal level. Conclusion: Glycerophospholipid metabolism may play an important role in the treatment of septic lung injury using XJDHT.
Isolated brachydactyly type E and idiopathic pancreatitis in a patient presenting to a lipid disorders clinic
