Complete genome sequencing and comparative genome analysis of the extremely halophilic archaea, Haloterrigena daqingensis

Author(s):  
Shuang Wang ◽  
Manik Prabhu Narsing Rao ◽  
Dan Wei ◽  
Lei Sun ◽  
Bao‐Zhu Fang ◽  
...  

2011 ◽  
Vol 86 (3) ◽  
pp. 1844-1852 ◽  
Author(s):  
A. Cornelissen ◽  
S. C. Hardies ◽  
O. V. Shaburova ◽  
V. N. Krylov ◽  
W. Mattheus ◽  
...  




2015 ◽  
Vol 37 (11) ◽  
pp. 959-968
Author(s):  
Jung Soo Seo ◽  
Mun Gyeong Kwon ◽  
Jee Youn Hwang ◽  
Sung Hee Jung ◽  
Hyun Ja Han ◽  
...  




2018 ◽  
Vol 66 (4) ◽  
pp. 352-359 ◽  
Author(s):  
P.H.N. Panzenhagen ◽  
C.C. Cabral ◽  
P.N. Suffys ◽  
R.M. Franco ◽  
D.P. Rodrigues ◽  
...  


2020 ◽  
Vol 7 (2) ◽  
pp. 74
Author(s):  
Longsheng Yang ◽  
Yongwei Zhu ◽  
Zhong Peng ◽  
Yi Ding ◽  
Kai Jie ◽  
...  

Erysipelothrix rhusiopathiae is a common pathogen responsible for pig erysipelas. However, the molecular basis for the pathogenesis of E. rhusiopathiae remains to be elucidated. In this study, the complete genome sequence of the E. rhusiopathiae strain WH13013, a pathogenic isolate from a diseased pig, was generated using a combined strategy of PacBio RSII and Illumina sequencing technologies. The strategy finally generated a single circular chromosome of approximately 1.78 Mb in size for the complete genome of WH13013, with an average GC content of 36.49%. The genome of WH13013 encoded 1633 predicted proteins, 55 tRNAs, as well as 15 rRNAs. It contained four genomic islands and several resistance-associated genes were identified within these islands. Phylogenetic analysis revealed that WH13013 was close to many other sequenced E. rhusiopathiae virulent strains. The comprehensive comparative analysis of eight E. rhusiopathiae virulent strains, including WH13013, identified a total of 1184 core genes. A large proportion (approximately 75.31%) of these core genes participated in nutrition and energy uptake and metabolism as well as the other bioactivities that are necessary for bacterial survival and adaption. The core genes also contained those encoding proteins participating in the biosynthesis and/or the components of the proposed virulence factors of E. rhusiopathiae, including the capsule (cpsA, cpsB, cpsC), neuraminidase (nanH), hyaluronidase (hylA, hylB, hylC), and surface proteins (spaA, rspA, rspB). The obtaining of the complete genome sequence of this virulent strain, WH13013, and this comprehensive comparative genome analysis will help in further studies of the genetic basis of the pathogenesis of E. rhusiopathiae.



2020 ◽  
Vol 16 ◽  
pp. 117693432094219
Author(s):  
Su Xu ◽  
Jianjun Cheng ◽  
Xiangchen Meng ◽  
Yan Xu ◽  
Ying Mu

Lactobacillus reuteri YSJL-12 was isolated from healthy sow fresh feces and used as probiotics additives previously. To investigate the genetic basis on probiotic potential and identify the genes in the strain, the complete genome of YSJL-12 was sequenced. Then comparative genome analysis on 9 strains of Lactobacillus reuteri was performed. The genome of YSJL-12 consisted of a circular 2,084,748 bp chromosome and 2 circular plasmids (51,906 and 15,134 bp). From among the 2065 protein-coding sequences (CDSs), the genes resistant to the environmental stress were identified. The function of COG (Clusters of Orthologous Group) protein genes was predicted, and the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways were analyzed. The comparative genome analysis indicated that the pan-genome contained a core genome of 1257 orthologous gene clusters, an accessory genome of 1064 orthologous gene clusters, and 1148 strain-specific genes, and the antibacterial mechanism among Lactobacillus reuteri strains might be different. The phylogenetic analysis and genomic collinearity revealed that the phylogenetic relationship among 9 strains of Lactobacillus reuteri was connected with host species and showed host specificity. The research could help us to better predict genes function and understand genetic basis on adapting to host gut in Lactobacillus reuteri YSJL-12.



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