Biosynthesis of phenylpyruvic acid from L‐phenylalanine using chromosomally engineered Escherichia coli

2021 ◽  
Author(s):  
Tianzhen Xiong ◽  
Yajun Bai ◽  
Tai‐Ping Fan ◽  
Xiaohui Zheng ◽  
Yujie Cai
Keyword(s):  
Escherichia Coli ◽  
Phenylpyruvic Acid ◽  
Engineered Escherichia Coli

scholarly journals Two-Step Production of Phenylpyruvic Acid from L-Phenylalanine by Growing and Resting Cells of Engineered Escherichia coli: Process Optimization and Kinetics Modeling

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0166457 ◽  
Author(s):  
Ying Hou ◽  
Gazi Sakir Hossain ◽  
Jianghua Li ◽  
Hyun-dong Shin ◽  
Long Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Process Optimization ◽  
Resting Cells ◽  
Phenylpyruvic Acid ◽  
Kinetics Modeling ◽  
Engineered Escherichia Coli

Biodecomposition of Phenanthrene and Pyrene by a Genetically Engineered Escherichia coli

2020 ◽  
Vol 14 (2) ◽  
pp. 121-133 ◽  
Author(s):  
Maryam Ahankoub ◽  
Gashtasb Mardani ◽  
Payam Ghasemi-Dehkordi ◽  
Ameneh Mehri-Ghahfarrokhi ◽  
Abbas Doosti ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Performance ◽  
Human Cancer ◽  
Genetically Engineered ◽  
Hazardous Substances ◽  
E Coli ◽  
Spiked Soil ◽  
Engineered Escherichia Coli ◽  
Genetically Manipulated ◽  
Hplc Measurement

Background: Genetically engineered microorganisms (GEMs) can be used for bioremediation of the biological pollutants into nonhazardous or less-hazardous substances, at lower cost. Polycyclic aromatic hydrocarbons (PAHs) are one of these contaminants that associated with a risk of human cancer development. Genetically engineered E. coli that encoded catechol 2,3- dioxygenase (C230) was created and investigated its ability to biodecomposition of phenanthrene and pyrene in spiked soil using high-performance liquid chromatography (HPLC) measurement. We revised patents documents relating to the use of GEMs for bioremediation. This approach have already been done in others studies although using other genes codifying for same catechol degradation approach. Objective: In this study, we investigated biodecomposition of phenanthrene and pyrene by a genetically engineered Escherichia coli. Methods: Briefly, following the cloning of C230 gene (nahH) into pUC18 vector and transformation into E. coli Top10F, the complementary tests, including catalase, oxidase and PCR were used as on isolated bacteria from spiked soil. Results: The results of HPLC measurement showed that in spiked soil containing engineered E. coli, biodegradation of phenanthrene and pyrene comparing to autoclaved soil that inoculated by wild type of E. coli and normal soil group with natural microbial flora, were statistically significant (p<0.05). Moreover, catalase test was positive while the oxidase tests were negative. Conclusion: These findings indicated that genetically manipulated E. coli can provide an effective clean-up process on PAH compounds and it is useful for bioremediation of environmental pollution with petrochemical products.

scholarly journals Highly efficient L-lactate production using engineered Escherichia coli with dissimilar temperature optima for L-lactate formation and cell growth

2014 ◽  
Vol 13 (1) ◽  
pp. 78 ◽  
Author(s):  
Dandan Niu ◽  
Kangming Tian ◽  
Bernard A Prior ◽  
Min Wang ◽  
Zhengxiang Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Lactate Production ◽  
Highly Efficient ◽  
Lactate Formation ◽  
Engineered Escherichia Coli ◽  
Temperature Optima

scholarly journals Production of Rainbow Colorants by Metabolically Engineered Escherichia coli

2021 ◽  
pp. 2100743
Author(s):  
Dongsoo Yang ◽  
Seon Young Park ◽  
Sang Yup Lee
Keyword(s):  
Escherichia Coli ◽  
Engineered Escherichia Coli ◽  
Metabolically Engineered Escherichia Coli

Conversion efficiency of bioethanol from levoglucosan was improved by the newly engineered Escherichia coli

2021 ◽  
Author(s):  
Dongdong Chang ◽  
Zia Ul Islam ◽  
Zhiguang Yang ◽  
Ian P. Thompson ◽  
Zhisheng Yu
Keyword(s):  
Escherichia Coli ◽  
Conversion Efficiency ◽  
Engineered Escherichia Coli

Biosynthesis of (R)-3-hydroxybutyric acid from syngas-derived acetate in engineered Escherichia coli

2021 ◽  
pp. 125323
Author(s):  
Peng Fei ◽  
Yuanchan Luo ◽  
Ningyu Lai ◽  
Hui Wu
Keyword(s):  
Escherichia Coli ◽  
Hydroxybutyric Acid ◽  
Engineered Escherichia Coli

scholarly journals Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber

2010 ◽  
Vol 107 (32) ◽  
pp. 14059-14063 ◽  
Author(s):  
X.-X. Xia ◽  
Z.-G. Qian ◽  
C. S. Ki ◽  
Y. H. Park ◽  
D. L. Kaplan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Spider Silk ◽  
Silk Protein ◽  
Spider Silk Protein ◽  
Engineered Escherichia Coli ◽  
Metabolically Engineered Escherichia Coli
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