Binding Equilibria of Multiazo Dyes with Polymeric Dye Transfer Inhibitors

1996 ◽  
Vol 100 (5) ◽  
pp. 661-670 ◽  
Author(s):  
Frank Runge ◽  
Jürgen Detering ◽  
Georg Zwissler ◽  
Dieter Boeckh ◽  
Christian Schade
Keyword(s):  
Dye Transfer ◽  
Polymeric Dye

Use of EDS mapping to characterize thermochemical stability of fibers in intermetallic matrix composites

1992 ◽  
Vol 50 (1) ◽  
pp. 160-161
Author(s):  
John R. Porter
Keyword(s):  
Spatial Information ◽  
Beam Current ◽  
Fiber Types ◽  
Ceramic Fibers ◽  
Dye Transfer ◽  
Science Center ◽  
Commercial Development ◽  
Nih Image ◽  
Intermetallic Matrix Composites ◽  
Thermochemical Stability

New ceramic fibers, currently in various stages of commercial development, have been consolidated in intermetallic matrices such as γ-TiAl and FeAl. Fiber types include SiC, TiB2 and polycrystalline and single crystal Al2O3. This work required the development of techniques to characterize the thermochemical stability of these fibers in different matrices.SEM/EDS elemental mapping was used for this work. To obtain qualitative compositional/spatial information, the best realistically achievable counting statistics were required. We established that 128 × 128 maps, acquired with a 20 KeV accelerating voltage, 3 sec. live time per pixel (total mapping time, 18 h) and with beam current adjusted to give 30% dead time, provided adequate image quality at a magnification of 800X. The maps were acquired, with backgrounds subtracted, using a Noran TN 5500 EDS system. The images and maps were transferred to a Macintosh and converted into TIFF files using either TIFF Maker, or TNtolMAGE, a Microsoft QuickBASIC program developed at the Science Center. From TIFF files, images and maps were opened in either NIH Image or Adobe Photoshop for processing and analysis and printed from Microsoft Powerpoint on a Kodak XL7700 dye transfer image printer.

A model for the role of the proline-linked pentose phosphate pathway in polymeric dye tolerance in oregano

2001 ◽  
Vol 36 (10) ◽  
pp. 941-946 ◽  
Author(s):  
Zuoxing Zheng ◽  
Urja Sheth ◽  
Mohan Nadiga ◽  
Jennifer L. Pinkham ◽  
Kalidas Shetty
Keyword(s):  
Pentose Phosphate Pathway ◽  
Pentose Phosphate ◽  
Polymeric Dye

Gap junction expression and cell proliferation in differentiating cultures of Cx43 KO mouse hepatocytes

2001 ◽  
Vol 281 (4) ◽  
pp. G1004-G1013 ◽  
Author(s):  
Takashi Kojima ◽  
Alfredo Fort ◽  
Mingyuan Tao ◽  
Masao Yamamoto ◽  
David C. Spray
Keyword(s):  
Lucifer Yellow ◽  
Primary Cultures ◽  
Mrna Levels ◽  
Wild Type ◽  
Dye Transfer ◽  
Gap Junctional Communication ◽  
Junctional Communication ◽  
Junctional Conductance ◽  
Mouse Hepatocytes

Primary cultures of adult mouse hepatocytes are shown here to reexpress differentiated hepatocyte features following treatment with 2% DMSO and 10−7 M glucagon. To examine the roles of gap junctional communication during hepatocyte growth and differentiation, we have compared treated and untreated hepatocytes from connexin (Cx)32-deficient [Cx32 knockout (KO)] and wild-type mice. In untreated cultures, DNA replication of Cx32 KO hepatocytes was markedly higher than of wild types. Although Cx26 mRNA levels remained high at all time points in wild-type and Cx32 KO hepatocytes, Cx32 mRNA and protein in wild-type hepatocytes underwent a marked decline, which recovered in 10-day treated cultures. Increased levels of Cx26 protein and junctional conductance were observed in Cx32 KO hepatocytes at 96 h in culture, a time when cell growth rate was high. Treatment with DMSO/glucagon highly reinduced Cx26 expression in Cx32 KO hepatocytes, and such treatment reinduced expression of both Cx32 and Cx26 expression in wild types. Dye transfer was not observed following Lucifer yellow injection into DMSO/glucagon-treated Cx32 KO hepatocytes, whereas the spread was extensive in wild types. Nevertheless, high junctional conductance values were observed in treated cells from both genotypes. These studies provide a method by which the differentiated phenotype can be obtained in cultured mouse hepatocytes and provide in vitro evidence that expression of gap junctions formed of Cx32 are involved in the regulation of growth of mouse hepatocytes.

scholarly journals Electrochemical DNA Sensor Based on Carbon Black—Poly(Neutral Red) Composite for Detection of Oxidative DNA Damage

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3489 ◽  
Author(s):  
Yurii Kuzin ◽  
Dominika Kappo ◽  
Anna Porfireva ◽  
Dmitry Shurpik ◽  
Ivan Stoikov ◽  
...  
Keyword(s):  
Carbon Black ◽  
Oxidative Dna Damage ◽  
Electrochemical Impedance ◽  
Neutral Red ◽  
Redox Activity ◽  
Antitumor Drugs ◽  
Oxygen Species ◽  
Dna Sensor ◽  
Electrochemical Dna Sensor ◽  
Polymeric Dye

Voltammetric DNA sensor has been proposed on the platform of glassy carbon electrode covered with carbon black with adsorbed pillar[5]arene molecules. Electropolymerization of Neutral Red performed in the presence of native or oxidatively damaged DNA resulted in formation of hybrid material which activity depended on the DNA conditions. The assembling of the surface layer was confirmed by scanning electron microscopy and electrochemical impedance spectroscopy. The influence of DNA and pillar[5]arene on redox activity of polymeric dye was investigated and a significant increase of the peak currents was found for DNA damaged by reactive oxygen species generated by Cu2+/H2O2 mixture. Pillar[5]arene improves the electron exchange conditions and increases the response and its reproducibility. The applicability of the DNA sensor developed was shown on the example of ascorbic acid as antioxidant. It decreases the current in the concentration range from 1.0 μM to 1.0 mM. The possibility to detect antioxidant activity was qualitatively confirmed by testing tera infusion. The DNA sensor developed can find application in testing of carcinogenic species and searching for new antitumor drugs.

Surface roughness induced random lasing in bio-polymeric dye doped film

2013 ◽  
Vol 576 ◽  
pp. 31-34 ◽  
Author(s):  
Lech Sznitko ◽  
Adam Szukalski ◽  
Konrad Cyprych ◽  
Pawel Karpinski ◽  
Andrzej Miniewicz ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Random Lasing ◽  
Polymeric Dye

scholarly journals Synthesis and Optical Performances of a Waterborne Polyurethane-Based Polymeric Dye

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Xianhai Hu ◽  
Xingyuan Zhang ◽  
Jin Liu
Keyword(s):  
Molecular Weight ◽  
Particle Size ◽  
Waterborne Polyurethane ◽  
Average Particle ◽  
Particle Sizes ◽  
Hypsochromic Shift ◽  
Absorption Bands ◽  
Polymeric Dye ◽  
Emulsion Particle ◽  
Increasing Temperature

A waterborne polyurethane-based polymeric dye (WPU-CFBB) was synthesized by anchoring 1, 4-bis(methylamino)anthraquinone (CFBB) to waterborne polyurethane chains. The number molecular weight, glass transition temperature, and average emulsion particle size for the polymeric dye were determined, respectively. This polymeric dye exhibited intriguing optical behaviors. The polymeric dye engendered two new absorption bands centered at about 520 nm and 760 nm if compared with CFBB in UV-vis spectra. The 760 nm peak showed hypsochromic shift with the decrease of average particle sizes. The polymeric dye dramatically demonstrated both hypsochromic and bathochromic effects with increasing temperature. The fluorescence intensity of the polymeric dye was much higher than that of CFBB. It was found that the fluorescence intensities would be enhanced from 20°C to 40°C and then decline from 40°C to 90°C. The fluorescence of the polymeric dye emulsion was very stable and was not sensitive to quenchers.

Scrape-loading/dye transfer technique associated with image analysis: A valuable quantitative method to assess tumor promotion

1992 ◽  
Vol 76 (2) ◽  
pp. 242-242
Author(s):  
Nizard Carine ◽  
Schoëvërt Damien ◽  
Adolphe Monique ◽  
Decloître Françoise
Keyword(s):  
Image Analysis ◽  
Quantitative Method ◽  
Tumor Promotion ◽  
Dye Transfer ◽  
Scrape Loading
Sign in / Sign up

Export Citation Format

Share Document