3D cell culture systems - towards primary drug discovery platforms: An interview with Heinz Ruffner (Novartis) and Jan Lichtenberg (InSphero)

2012 ◽  
Vol 7 (7) ◽  
pp. 833-834
Author(s):  
Uta Göbel
Keyword(s):  
Cell Culture ◽  
Drug Discovery ◽  
3D Cell Culture ◽  
Culture Systems ◽  
Cell Culture Systems ◽  
Primary Drug

scholarly journals Mammary gland 3D cell culture systems in farm animals

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Laurence Finot ◽  
Eric Chanat ◽  
Frederic Dessauge
Keyword(s):  
Cell Culture ◽  
Mammary Gland ◽  
Bacterial Infections ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Farm Animals ◽  
Culture Systems ◽  
Cell Culture Systems

AbstractIn vivo study of tissue or organ biology in mammals is very complex and progress is slowed by poor accessibility of samples and ethical concerns. Fortunately, however, advances in stem cell identification and culture have made it possible to derive in vitro 3D “tissues” called organoids, these three-dimensional structures partly or fully mimicking the in vivo functioning of organs. The mammary gland produces milk, the source of nutrition for newborn mammals. Milk is synthesized and secreted by the differentiated polarized mammary epithelial cells of the gland. Reconstructing in vitro a mammary-like structure mimicking the functional tissue represents a major challenge in mammary gland biology, especially for farm animals for which specific agronomic questions arise. This would greatly facilitate the study of mammary gland development, milk secretion processes and pathological effects of viral or bacterial infections at the cellular level, all with the objective of improving milk production at the animal level. With this aim, various 3D cell culture models have been developed such as mammospheres and, more recently, efforts to develop organoids in vitro have been considerable. Researchers are now starting to draw inspiration from other fields, such as bioengineering, to generate organoids that would be more physiologically relevant. In this chapter, we will discuss 3D cell culture systems as organoids and their relevance for agronomic research.

3D Cell Culture Systems for the Development of Neural Interfaces

2020 ◽  
pp. 201-236
Author(s):  
Omaer Syed ◽  
Chris Chapman ◽  
Catalina Vallejo-Giraldo ◽  
Martina Genta ◽  
Josef Goding ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Neural Interfaces ◽  
Culture Systems ◽  
Cell Culture Systems

scholarly journals Human iPS Cell-Derived Patient Tissues and 3D Cell Culture Part 2: Spheroids, Organoids, and Disease Modeling

2019 ◽  
Vol 24 (1) ◽  
pp. 18-27 ◽  
Author(s):  
Richard M. Eglen ◽  
Terry Reisine
Keyword(s):  
Cell Culture ◽  
Drug Discovery ◽  
Disease Modeling ◽  
Cortical Cell ◽  
Neuronal Cell ◽  
3D Cell Culture ◽  
Retinal Cell ◽  
Major Advance ◽  
Ips Cell ◽  
Culture Systems

Human induced pluripotent stem cells (HiPSCs) provide several advantages for drug discovery, but principally they provide a source of clinically relevant tissue. Furthermore, the use of HiPSCs cultured in three-dimensional (3D) systems, as opposed to traditional two-dimensional (2D) culture approaches, better represents the complex tissue architecture in vivo. The use of HiPSCs in 3D spheroid and organoid culture is now growing, but particularly when using myocardial, intestinal enteric nervous system, and retinal cell lines. However, organoid cell culture is perhaps making the most notable impact in research and drug discovery, in which 3D neuronal cell cultures allow direct modeling of cortical cell layering and neuronal circuit activity. Given the specific degeneration seen in discrete neuronal circuitry in Alzheimer’s disease (AD) and Parkinson’s disease (PD), HiPSC culture systems are proving to be a major advance. In the present review, the second part of a two-part review, we discuss novel methods in which 3D cell culture systems (principally organoids) are now being used to provide insights into disease mechanisms. (The use of HiPSCs in target identification was reviewed in detail in Part 1.)

scholarly journals Biophysically Defined and Cytocompatible Covalently Adaptable Networks as Viscoelastic 3D Cell Culture Systems

2013 ◽  
Vol 26 (6) ◽  
pp. 865-872 ◽  
Author(s):  
Daniel D. McKinnon ◽  
Dylan W. Domaille ◽  
Jennifer N. Cha ◽  
Kristi S. Anseth
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Culture Systems ◽  
Cell Culture Systems

Antitumor activity of amidino-substituted benzimidazole and benzimidazo[1,2-a]quinoline derivatives tested in 2D and 3D cell culture systems

2015 ◽  
Vol 31 (6) ◽  
pp. 1139-1145 ◽  
Author(s):  
Karmen Brajša ◽  
Ines Vujasinović ◽  
Dubravko Jelić ◽  
Marija Trzun ◽  
Ivo Zlatar ◽  
...  
Keyword(s):  
Cell Culture ◽  
Antitumor Activity ◽  
3D Cell Culture ◽  
Quinoline Derivatives ◽  
Culture Systems ◽  
Cell Culture Systems ◽  
2D And 3D
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