In Situ Bone Tissue Engineering With an Endogenous Stem Cell Mobilizer and Osteoinductive Nanofibrous Polymeric Scaffolds

Over the past decades, a number of bone tissue engineering (BTE) approaches have been developed to address substantial challenges in the management of critical size bone defects. Although the majority of BTE strategies developed in the laboratory have been limited due to lack of clinical relevance in translation, primary prerequisites for the construction of vascularized functional bone grafts have gained confidence owing to the accumulated knowledge of the osteogenic, osteoinductive, and osteoconductive properties of mesenchymal stem cells and bone-relevant biomaterials that reflect bone-healing mechanisms. In this review, we summarize the current knowledge of bone-healing mechanisms focusing on the details that should be embodied in the development of vascularized BTE, and discuss promising strategies based on 3D-bioprinting technologies that efficiently coalesce the abovementioned main features in bone-healing systems, which comprehensively interact during the bone regeneration processes.

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Synthesis and Characterization of Novel Injectable, Biodegradable and In situ Crosslinkable Poly(hexamethylene-carbonate-fumarate), Poly(hexamethylene carbonate) Diacrylate and Poly(ethylene glycol fumarate-co-hexamethylene carbonate-fumarate) Scaffolds for Bone Tissue Engineering

2006 International Conference of the IEEE Engineering in Medicine and Biology Society ◽

10.1109/iembs.2006.259887 ◽

2006 ◽

Cited By ~ 4

Author(s):

S. Sharifi ◽

H. Mirzadeh ◽

M. Imani ◽

M. Atai ◽

R. Bakhshi ◽

...

Keyword(s):

Tissue Engineering ◽

Ethylene Glycol ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Synthesis And Characterization ◽

Poly Ethylene Glycol ◽

Poly Ethylene

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Role of Interfacial Interactions on Mechanical Properties of Biomimetic Composites for Bone Tissue Engineering

MRS Proceedings ◽

10.1557/proc-0898-l08-03 ◽

2005 ◽

Vol 898 ◽

Cited By ~ 1

Author(s):

Devendra Verma ◽

Rahul Bhowmik ◽

Bedabibhas Mohanty ◽

Dinesh R Katti ◽

Kalpana S Katti

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Mechanical Response ◽

Density Ratio ◽

Interfacial Interactions ◽

Porous Composites ◽

Properties Of Composites

AbstractInterfaces play an important role in controlling the mechanical properties of composites. Optimum mechanical strength of scaffolds is of prime importance for bone tissue engineering. In the present work, molecular dynamics simulations and experimental studies have been conducted to study effect of interfacial interactions on mechanical properties of composites for bone replacement. In order to mimic biological processes, hydroxyapatite (HAP) is mineralized in presence of polyacrylic acid (PAAc) (in situ HAP). Further, solid and porous composites of in situ HAP with polycaprolactone (PCL) are made. Mechanical tests of composites of in situ HAP with PAAc have shown improved strain recovery, higher modulus/density ratio and also improved mechanical response in simulated body fluid (SBF). Simulation studies indicate potential for calcium bridging between –COO− of PAAc and surface calcium of HAP. This fact is also supported by infrared spectroscopic studies. PAAc modified surfaces of in situ HAP offer means to control the microstructure and mechanical response of porous composites. Nanoindentation experiments indicate that apatite grown on in situ HAP/PCL composites from SBF has improved elastic modulus and hardness. This work gives insight into the interfacial mechanisms responsible for mechanical response as well as bioactivity in biomaterials.

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Osteon-mimetic 3D nanofibrous scaffold enhancing stem cell proliferation and osteogenic differentiation for bone regeneration

Biomaterials Science ◽

10.1039/d1bm01489g ◽

2022 ◽

Author(s):

Ting Song ◽

Jianhua Zhou ◽

Ming Shi ◽

Liuyang Xuan ◽

Huamin Jiang ◽

...

Keyword(s):

Tissue Engineering ◽

Cell Proliferation ◽

Stem Cell ◽

Bone Tissue Engineering ◽

Bone Regeneration ◽

Osteogenic Differentiation ◽

Bone Tissue ◽

Nanofibrous Scaffold ◽

Hierarchical Microstructure ◽

Stem Cell Proliferation

Scaffold microstructure is important for bone tissue engineering. Failure to synergistically imitate the hierarchical microstructure of bone component, such as osteon with concentric multilayers assembled by nanofibers, hindered the performance...

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Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach

Biomedical Materials ◽

10.1088/1748-605x/ac355a ◽

2021 ◽

Vol 17 (1) ◽

pp. 015003

Author(s):

Lya Piaia ◽

Simone S Silva ◽

Joana M Gomes ◽

Albina R Franco ◽

Emanuel M Fernandes ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Regeneration ◽

Bone Tissue ◽

Silk Fibroin ◽

Cellular Proliferation ◽

Mechanical Performance ◽

Tissue Growth ◽

Polymeric Scaffolds ◽

Bioactive Agents

Abstract Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth. By introducing scaffolds that can be autografts and/or allografts, tissue engineering provides new approaches to manage the major burdens involved in this process. Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions. The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering. Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60–170 μm, and a stiffness ranging from 1 to 2 MPa. Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture. Besides, cellular proliferation was higher on the scaffolds coated with silk. These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.

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