Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. III. Utilization of two thermostable ?-glycosidases in a continuous ultrafiltration membrane reactor and galacto-oligosaccharide formation under steady-state conditions

2002 ◽  
Vol 77 (4) ◽  
pp. 394-404 ◽  
Author(s):  
Inge Petzelbauer ◽  
Barbara Splechtna ◽  
Bernd Nidetzky
2015 ◽  
Vol 494 ◽  
pp. 182-187 ◽  
Author(s):  
Markus Barth ◽  
Ren Wei ◽  
Thorsten Oeser ◽  
Johannes Then ◽  
Juliane Schmidt ◽  
...  

1984 ◽  
Vol 26 (7) ◽  
pp. 647-653 ◽  
Author(s):  
Ingrid Ohlson ◽  
Gun Trägårdh ◽  
Bärbel Hahn-Hägerdal

1983 ◽  
Vol 16 ◽  
pp. 407-416 ◽  
Author(s):  
Francesco Alfani ◽  
Maria Cantarella ◽  
Vincenzo Scardi

2010 ◽  
Vol 15 (6) ◽  
pp. 917-922 ◽  
Author(s):  
Zbyszko Lubiewski ◽  
Joanna Le Thanh ◽  
Grażyna Lewandowicz

Processes ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1703
Author(s):  
Mukhtar Nuhu Yahya ◽  
Hüseyin Gökçekuş ◽  
Derin Orhon ◽  
Bülent Keskinler ◽  
Ahmet Karagunduz ◽  
...  

1. Background: Urea is the main product of the nitrogenous breakdown of protein metabolism in mammals. In this study, process intensification for enzymatic hydrolysis of urea by urease enzyme (jack bean urease) was examined in a membrane reactor. 2. Methods: Batch and continuous enzymatic hydrolysis reactions were performed at different substrate concentrations to determine the digestibility and affinity of the substrate with that of the enzyme. The hydrolysate samples were obtained by an optimized continuous enzyme membrane reactor (EMR) coupled with an ultra-filtration membrane (250 kDa). Feed concentration varied from 100 to 500 mg/L. Laboratory experiments were conducted at room temperature (20 ± 1 °C), with a flow rate of 20 mL/min, urease concentration of 0.067 g/L, ionic strength (I = 0, 0.01, 0.05), and ammonium nitrogen addition of (0, 100 mg/L, 200 mg/L, 500 mg/L). Moreover, the effect of ionic strength, ammonium nitrogen concentration, feed concentration, and enzyme concentration on urea hydrolysis was examined. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDAX) analysis were used to identify the physicochemical properties as well as the elemental composition of the Ultra-Filtration membrane used in this study. 3. Results: The study revealed that higher ionic strength and higher concentrations of NH4SO2 and ammonium nitrogen (NH3-N) inhibithydrolysis of urea by reducing the urease enzyme activity in the system over time. 4. Conclusions: Herein, a sustainable alternative for the conversion of urea to ammonia by utilizing urease in an EMR was demonstrated.


2017 ◽  
Vol 12 ◽  
pp. 50-58 ◽  
Author(s):  
Thaothy Nguyenhuynh ◽  
Rajesh Nithyanandam ◽  
Chien Hwa Chong ◽  
Duduku Krishnaiah

Desalination ◽  
2012 ◽  
Vol 284 ◽  
pp. 309-315 ◽  
Author(s):  
Hua Hang ◽  
Wanmeng Mu ◽  
Bo Jiang ◽  
Meng Zhao ◽  
Liuming (Leon) Zhou ◽  
...  

1990 ◽  
Vol 54 (11) ◽  
pp. 2793-2799
Author(s):  
Mitsutoshi Nakajima ◽  
Ken-ichi Iwasaki ◽  
Hiroshi Nabetani ◽  
Atsuo Watanabe

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