Enzymatic hydrolysis of polyethylene terephthalate films in an ultrafiltration membrane reactor

2015 ◽  
Vol 494 ◽  
pp. 182-187 ◽  
Author(s):  
Markus Barth ◽  
Ren Wei ◽  
Thorsten Oeser ◽  
Johannes Then ◽  
Juliane Schmidt ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Polyethylene Terephthalate ◽  
Membrane Reactor ◽  
Ultrafiltration Membrane ◽  
Hydrolysis Of

Enzymatic hydrolysis of sodium-hydroxide-pretreated sallow in an ultrafiltration membrane reactor

1984 ◽  
Vol 26 (7) ◽  
pp. 647-653 ◽  
Author(s):  
Ingrid Ohlson ◽  
Gun Trägårdh ◽  
Bärbel Hahn-Hägerdal
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sodium Hydroxide ◽  
Membrane Reactor ◽  
Ultrafiltration Membrane ◽  
Hydrolysis Of

Use of a membrane reactor for studying enzymatic hydrolysis of cellulose

1983 ◽  
Vol 16 ◽  
pp. 407-416 ◽  
Author(s):  
Francesco Alfani ◽  
Maria Cantarella ◽  
Vincenzo Scardi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Membrane Reactor ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Increasing the efficiency of the enzymatic hydrolysis of potato starch in a membrane reactor

2010 ◽  
Vol 15 (6) ◽  
pp. 917-922 ◽  
Author(s):  
Zbyszko Lubiewski ◽  
Joanna Le Thanh ◽  
Grażyna Lewandowicz
Keyword(s):  
Enzymatic Hydrolysis ◽  
Membrane Reactor ◽  
Potato Starch ◽  
Hydrolysis Of

Turbidimetric analysis of the enzymatic hydrolysis of polyethylene terephthalate nanoparticles

2014 ◽  
Vol 103 ◽  
pp. 72-78 ◽  
Author(s):  
Ren Wei ◽  
Thorsten Oeser ◽  
Markus Barth ◽  
Nancy Weigl ◽  
Anja Lübs ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Polyethylene Terephthalate ◽  
Turbidimetric Analysis ◽  
Hydrolysis Of

scholarly journals A Study on the Hydrolysis of Urea Contained in Wastewater and Continuous Recovery of Ammonia by an Enzymatic Membrane Reactor

Processes ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1703
Author(s):  
Mukhtar Nuhu Yahya ◽  
Hüseyin Gökçekuş ◽  
Derin Orhon ◽  
Bülent Keskinler ◽  
Ahmet Karagunduz ◽  
...  
Keyword(s):  
Ionic Strength ◽  
Enzymatic Hydrolysis ◽  
Membrane Reactor ◽  
Ammonium Nitrogen ◽  
Feed Concentration ◽  
Filtration Membrane ◽  
Urease Enzyme ◽  
Ultra Filtration ◽  
Hydrolysis Of ◽  
Hydrolysis Of Urea

1. Background: Urea is the main product of the nitrogenous breakdown of protein metabolism in mammals. In this study, process intensification for enzymatic hydrolysis of urea by urease enzyme (jack bean urease) was examined in a membrane reactor. 2. Methods: Batch and continuous enzymatic hydrolysis reactions were performed at different substrate concentrations to determine the digestibility and affinity of the substrate with that of the enzyme. The hydrolysate samples were obtained by an optimized continuous enzyme membrane reactor (EMR) coupled with an ultra-filtration membrane (250 kDa). Feed concentration varied from 100 to 500 mg/L. Laboratory experiments were conducted at room temperature (20 ± 1 °C), with a flow rate of 20 mL/min, urease concentration of 0.067 g/L, ionic strength (I = 0, 0.01, 0.05), and ammonium nitrogen addition of (0, 100 mg/L, 200 mg/L, 500 mg/L). Moreover, the effect of ionic strength, ammonium nitrogen concentration, feed concentration, and enzyme concentration on urea hydrolysis was examined. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDAX) analysis were used to identify the physicochemical properties as well as the elemental composition of the Ultra-Filtration membrane used in this study. 3. Results: The study revealed that higher ionic strength and higher concentrations of NH4SO2 and ammonium nitrogen (NH3-N) inhibithydrolysis of urea by reducing the urease enzyme activity in the system over time. 4. Conclusions: Herein, a sustainable alternative for the conversion of urea to ammonia by utilizing urease in an EMR was demonstrated.

Configuration modification of a submerged membrane reactor for enzymatic hydrolysis of cellulose

2017 ◽  
Vol 12 ◽  
pp. 50-58 ◽  
Author(s):  
Thaothy Nguyenhuynh ◽  
Rajesh Nithyanandam ◽  
Chien Hwa Chong ◽  
Duduku Krishnaiah
Keyword(s):  
Enzymatic Hydrolysis ◽  
Membrane Reactor ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Enzymatic hydrolysis of inulin in a bioreactor coupled with an ultrafiltration membrane

Desalination ◽  
2012 ◽  
Vol 284 ◽  
pp. 309-315 ◽  
Author(s):  
Hua Hang ◽  
Wanmeng Mu ◽  
Bo Jiang ◽  
Meng Zhao ◽  
Liuming (Leon) Zhou ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ultrafiltration Membrane ◽  
Hydrolysis Of

scholarly journals Fusion of Chitin-Binding Domain From Chitinolyticbacter meiyuanensis SYBC-H1 to the Leaf-Branch Compost Cutinase for Enhanced PET Hydrolysis

2021 ◽  
Vol 9 ◽  
Author(s):  
Rui Xue ◽  
Yinping Chen ◽  
Huan Rong ◽  
Ren Wei ◽  
Zhongli Cui ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Enzymatic Hydrolysis ◽  
Polyethylene Terephthalate ◽  
Binding Domain ◽  
Plastic Pollution ◽  
Pet Waste ◽  
Chitin Binding Domain ◽  
C Terminus ◽  
Measurable Activity ◽  
Hydrolysis Of

Polyethylene terephthalate (PET) is a mass-produced petroleum-based non-biodegradable plastic that contributes to the global plastic pollution. Recently, biocatalytic degradation has emerged as a viable recycling approach for PET waste, especially with thermophilic polyester hydrolases such as a cutinase (LCC) isolated from a leaf-branch compost metagenome and its variants. To improve the enzymatic PET hydrolysis performance, we fused a chitin-binding domain (ChBD) from Chitinolyticbacter meiyuanensis SYBC-H1 to the C-terminus of the previously reported LCCICCG variant, demonstrating higher adsorption to PET substrates and, as a result, improved degradation performance by up to 19.6% compared to with its precursor enzyme without the binding module. For compare hydrolysis with different binding module, the catalytic activity of LCCICCG-ChBD, LCCICCG-CBM, LCCICCG-PBM and LCCICCG-HFB4 were further investigated with PET substrates of various crystallinity and it showed measurable activity on high crystalline PET with 40% crystallinity. These results indicated that fusing a polymer-binding module to LCCICCG is a promising method stimulating the enzymatic hydrolysis of PET.

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