A combined method for determining inhibition type, kinetic parameters, and inhibition coefficients for aerobic cometabolism of 1,1,1-trichloroethane by a butane-grown mixed culture

2002 ◽  
Vol 77 (5) ◽  
pp. 564-576 ◽  
Author(s):  
Young Kim ◽  
Daniel J. Arp ◽  
Lewis Semprini
Keyword(s):  
Kinetic Parameters ◽  
Mixed Culture ◽  
Combined Method ◽  
Aerobic Cometabolism

Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane-grown mixed culture

2005 ◽  
Vol 52 (8) ◽  
pp. 125-131 ◽  
Author(s):  
Y. Kim ◽  
L. Semprini
Keyword(s):  
Mass Spectrometry ◽  
Mixed Culture ◽  
Mercuric Chloride ◽  
Aerobic Cometabolism ◽  
Release Studies ◽  
Kinetic Tests ◽  
Biological Transformation ◽  
Butane Monooxygenase ◽  
Grown Culture ◽  
Charge Fragment

Aerobic cometabolism of cis-1,2-dichloroethylene (c-DCE) by a butane-grown mixed culture was evaluated in batch kinetic tests. The transformation of c-DCE resulted in the coincident generation of c-DCE epoxide. Chloride release studies showed ∼75% oxidative dechlorination of c-DCE. Mass spectrometry confirmed the presence of a compound with mass-to-charge-fragment ratios of 112, 83, 48, and 35. These values are in agreement with the spectra of chemically synthesized c-DCE epoxide. The transformation of c-DCE required O2, was inhibited by butane and was inactivated by acetylene (a known monooxygenase inactivator), indicating that a butane monooxygenase enzyme was likely involved in the transformation of c-DCE. This study showed c-DCE epoxide was biologically transformed, likely by a butane monooxygenase enzyme. c-DCE epoxide transformation was inhibited by both acetylene and c-DCE indicating a monooxygenase enzyme was involved. The epoxide transformation was also stopped when mercuric chloride (HgCl2) was added as a biological inhibitor, further support a biological transformation. To our knowledge this is the first report of the biological transform c-DCE epoxide by a butane-grown culture.

Kinetic parameters of a mixed culture oxidizing sulfide and sulfur with oxygen

1991 ◽  
Vol 38 (8) ◽  
pp. 813-820 ◽  
Author(s):  
Cees J. N. Buisman ◽  
Peter I Jspeert ◽  
Anne Hof ◽  
Albert J. H. Janssen ◽  
Robert Ten Hagen ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Mixed Culture

Biodegradation of aliphatic and aromatic hydrocarbons at high temperatures

2003 ◽  
Vol 47 (10) ◽  
pp. 123-130 ◽  
Author(s):  
H. Feitkenhauer ◽  
H. Märkl
Keyword(s):  
High Temperature ◽  
Kinetic Parameters ◽  
Mixed Culture ◽  
High Temperatures ◽  
Aromatic Hydrocarbons ◽  
Chemical System ◽  
Separate Experiment ◽  
Phenol Biodegradation ◽  
Aliphatic And Aromatic Hydrocarbons ◽  
Very High

In this paper, the high temperature (65-75°C) biodegradation of aliphatic and aromatic hydrocarbons is investigated and kinetic parameters are derived. The shift of the physico-chemical system properties with rising temperature will be discussed in detail. For example, the solubility of naphthalene is increased by a factor of about ten if the temperature is increased from 20 to 75°C. This effect is essential to increase the bioavailability of sparingly soluble hydrocarbons. It is also demonstrated in experiments that very high oxygen transfer rates can be obtained at high temperatures in the presence of hydrocarbons. It is shown that efficient phenol biodegradation is essential for high temperature hydrocarbon degradation because some microorganisms tend to transform phenols into polyphenols which are very inhibitory for microbial growth. A defined mixed culture adapted to phenol converted more than 90% of a mixture of phenol, hexadecane and pyrene and a very high maximal growth rate of 0.19 h−1 was determined. A yield coefficient YX/S of about 0.8 g (biomass)/g (hydrocarbons) was calculated in this experiment. In a separate experiment the influence of the hydrocarbon droplet size on the biodegradation is investigated at 70°C using a newly isolated Thermus sp. In this case, the growth on a hexadecane/pyrene mixture was described by a model based on the Monod equation and the corresponding kinetic parameters are derived. A mixed culture was used for the bioremediation of soil in a slurry reactor. The initial contamination of 11 g/kg was lowered to about 2 g in a reactor inoculated by an immobilized culture of extreme thermophilic microorganisms, while 9 g/kg remained in a sterile control.

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