Antibody production by a hybridoma cell line at high cell density is limited by two independent mechanisms

2002 ◽  
Vol 79 (3) ◽  
pp. 277-283 ◽  
Author(s):  
Michael J. Gramer ◽  
Tracey L. Britton
Keyword(s):  
Cell Line ◽  
Cell Density ◽  
Antibody Production ◽  
High Cell Density ◽  
High Cell

scholarly journals Alternative patterns of mitogenesis and cell scattering induced by acidic FGF as a function of cell density in a rat bladder carcinoma cell line.

1990 ◽  
Vol 1 (13) ◽  
pp. 975-988 ◽  
Author(s):  
A M Vallés ◽  
G C Tucker ◽  
J P Thiery ◽  
B Boyer
Keyword(s):  
Cell Line ◽  
Cell Density ◽  
Bladder Carcinoma ◽  
Carcinoma Cell ◽  
High Cell Density ◽  
Carcinoma Cell Line ◽  
Dual Function ◽  
High Cell ◽  
Rat Bladder ◽  
Bladder Carcinoma Cell Line

The dual function exerted by acidic fibroblast growth factor (aFGF) in a rat bladder carcinoma cell line has been explored under two different conditions of culture density. At low cell density, aFGF promotes the epithelium-to-mesenchyme transition of NBT-II cells characterized by cell dissociation, morphological changes toward a fibroblastic-like phenotype, and acquisition of cell motility. Under these conditions, NBT-II cells are unresponsive to the growth-promoting effect of aFGF. At high cell density, aFGF is a potent mitogenic factor, but its scattering activity is essentially abrogated. Slight modifications in the binding of aFGF to its specific receptors were observed at high cell density; these changes correlated with a downregulation of receptors with no apparent change in their molecular form. NBT-II cells located at the edge of artificial wounds mimicked the behavior of subconfluent cells, because they did not proliferate upon aFGF treatment. Furthermore, in large-sized NBT-II colonies, peripheral cells were the first to dissociate in response to aFGF. Altogether, our results suggest that the cellular response to multifunctional growth factors might depend on the localization within the responding cell population.

scholarly journals Cell line development for continuous high cell density biomanufacturing: Exploiting hypoxia for improved productivity

2021 ◽  
pp. e00181
Author(s):  
Nikolas Zeh ◽  
Patrick Schlossbauer ◽  
Nadja Raab ◽  
Florian Klingler ◽  
René Handrick ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Density ◽  
High Cell Density ◽  
High Cell ◽  
Cell Line Development

scholarly journals Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture

2004 ◽  
Vol 44 (3) ◽  
pp. 93-102 ◽  
Author(s):  
Volker M. Gorenflo ◽  
Tom A. Pfeifer ◽  
Gary Lesnicki ◽  
Emily M. Kwan ◽  
Thomas A. Grigliatti ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Cell Line ◽  
Cell Density ◽  
Insect Cell ◽  
High Cell Density ◽  
Insect Cell Line ◽  
Perfusion Culture ◽  
Factor X ◽  
High Cell

scholarly journals Efficient influenza A virus production in high cell density using the novel porcine suspension cell line PBG.PK2.1

Vaccine ◽  
2019 ◽  
Vol 37 (47) ◽  
pp. 7019-7028 ◽  
Author(s):  
Gwendal Gränicher ◽  
Juliana Coronel ◽  
Alexander Pralow ◽  
Pavel Marichal-Gallardo ◽  
Michael Wolff ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Density ◽  
Influenza A Virus ◽  
Influenza A ◽  
High Cell Density ◽  
Virus Production ◽  
Suspension Cell ◽  
The Novel ◽  
High Cell ◽  
Suspension Cell Line
Sign in / Sign up

Export Citation Format

Share Document