Fed-batch mode in shake flasks by slow-release technique

2006 ◽  
Vol 95 (3) ◽  
pp. 433-445 ◽  
Author(s):  
M. Jeude ◽  
B. Dittrich ◽  
H. Niederschulte ◽  
T. Anderlei ◽  
C. Knocke ◽  
...  
Keyword(s):  
Slow Release ◽  
Fed Batch ◽  
Batch Mode ◽  
Release Technique ◽  
Shake Flasks

scholarly journals OPTIMIZATION OF RIFAMYCIN B USING NOCARDIA MEDITERRANEA (ATCC 13865) BY FED BATCH MODE IN SHAKE FLASKS

2017 ◽  
Vol 7 (4) ◽  
Author(s):  
Suneel Kumar Niranjan ◽  
Chandrul Kaushal ◽  
S K Jain
Keyword(s):  
Fed Batch ◽  
Batch Mode ◽  
Shake Flasks ◽  
Rifamycin B

scholarly journals Ustilago Rabenhorstiana—An Alternative Natural Itaconic Acid Producer

Fermentation ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 4 ◽  
Author(s):  
Susan Krull ◽  
Malin Lünsmann ◽  
Ulf Prüße ◽  
Anja Kuenz
Keyword(s):  
Itaconic Acid ◽  
Aspergillus Terreus ◽  
Degradation Products ◽  
Basidiomycetous Yeasts ◽  
Fed Batch ◽  
Batch Mode ◽  
Renewable Feedstocks ◽  
The Family ◽  
Sugar Degradation ◽  
Shake Flasks

Itaconic acid is an industrial produced chemical by the sensitive filamentous fungus Aspergillus terreus and can replace petrochemical-based monomers for polymer industry. To produce itaconic acid with alternative renewable substrates, such as lignocellulosic based hydrolysates, a robust microorganism is needed due to varying compositions and impurities. Itaconic acid producing basidiomycetous yeasts of the family Ustilaginaceae provide this required characteristic and the species Ustilago rabenhorstiana was examined in this study. By an optimization of media components, process parameters, and a fed-batch mode with glucose the final titer increased from maximum 33.3 g·L−1 in shake flasks to 50.3 g·L−1 in a bioreactor. Moreover, itaconic acid was produced from different sugar monomers based on renewable feedstocks by U. rabenhorstiana and the robustness against weak acids as sugar degradation products was confirmed. Based on these findings, U. rabenhorstiana has a high potential as alternative natural itaconic acid producer besides the well-known U. maydis and A. terreus.

scholarly journals Optimization of industrial production of rifamycin B by Amycolatopsis mediterranei. III. Production in fed-batch mode in shake flasks

2004 ◽  
Vol 3 (8) ◽  
pp. 387-394 ◽  
Author(s):  
O.M. El-Tayeb ◽  
A. A. Salama ◽  
M.M. Hussein ◽  
H.F El-Sedawy
Keyword(s):  
Industrial Production ◽  
Amycolatopsis Mediterranei ◽  
Fed Batch ◽  
Batch Mode ◽  
Shake Flasks ◽  
Rifamycin B

scholarly journals Screening for optimal protease producing Bacillus licheniformis strains with polymer-based controlled-release fed-batch microtiter plates

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Tobias Habicher ◽  
Tobias Klein ◽  
Jacqueline Becker ◽  
Andreas Daub ◽  
Jochen Büchs
Keyword(s):  
Controlled Release ◽  
Bacillus Licheniformis ◽  
Protease Activity ◽  
Microtiter Plate ◽  
Fed Batch ◽  
Batch Mode ◽  
Screening Process ◽  
Microtiter Plates ◽  
Batch Fermenter ◽  
Production Conditions

Abstract Background Substrate-limited fed-batch conditions have the favorable effect of preventing overflow metabolism, catabolite repression, oxygen limitation or inhibition caused by elevated substrate or osmotic concentrations. Due to these favorable effects, fed-batch mode is predominantly used in industrial production processes. In contrast, screening processes are usually performed in microtiter plates operated in batch mode. This leads to a different physiological state of the production organism in early screening and can misguide the selection of potential production strains. To close the gap between screening and production conditions, new techniques to enable fed-batch mode in microtiter plates have been described. One of these systems is the ready-to-use and disposable polymer-based controlled-release fed-batch microtiter plate (fed-batch MTP). In this work, the fed-batch MTP was applied to establish a glucose-limited fed-batch screening procedure for industrially relevant protease producing Bacillus licheniformis strains. Results To achieve equal initial growth conditions for different clones with the fed-batch MTP, a two-step batch preculture procedure was developed. Based on this preculture procedure, the standard deviation of the protease activity of glucose-limited fed-batch main culture cultivations in the fed-batch MTP was ± 10%. The determination of the number of replicates revealed that a minimum of 6 parallel cultivations were necessary to identify clones with a statistically significant increased or decreased protease activity. The developed glucose-limited fed-batch screening procedure was applied to 13 industrially-relevant clones from two B. licheniformis strain lineages. It was found that 12 out of 13 clones (92%) were classified similarly as in a lab-scale fed-batch fermenter process operated under glucose-limited conditions. When the microtiter plate screening process was performed in batch mode, only 5 out of 13 clones (38%) were classified similarly as in the lab-scale fed-batch fermenter process. Conclusion The glucose-limited fed-batch screening process outperformed the usual batch screening process in terms of the predictability of the clone performance under glucose-limited fed-batch fermenter conditions. These results highlight that the implementation of glucose-limited fed-batch conditions already in microtiter plate scale is crucial to increase the precision of identifying improved protease producing B. licheniformis strains. Hence, the fed-batch MTP represents an efficient high-throughput screening tool that aims at closing the gap between screening and production conditions.

Equalizing growth in high-throughput small scale cultivations via precultures operated in fed-batch mode

2009 ◽  
Vol 103 (6) ◽  
pp. 1095-1102 ◽  
Author(s):  
Robert Huber ◽  
Marco Scheidle ◽  
Barbara Dittrich ◽  
Doris Klee ◽  
Jochen Büchs
Keyword(s):  
High Throughput ◽  
Small Scale ◽  
Fed Batch ◽  
Batch Mode

Solar composting greenhouse for organic waste treatment in fed-batch mode: Physicochemical and microbiological dynamics

2020 ◽  
Vol 113 ◽  
pp. 1-11 ◽  
Author(s):  
Hui Lin ◽  
Jing Ye ◽  
Wanchun Sun ◽  
Qiaogang Yu ◽  
Qiang Wang ◽  
...  
Keyword(s):  
Organic Waste ◽  
Waste Treatment ◽  
Fed Batch ◽  
Batch Mode ◽  
Organic Waste Treatment

A slow-release technique for inducing prolonged paralysis by tetrodotoxin

1979 ◽  
Vol 383 (1) ◽  
pp. 67-70 ◽  
Author(s):  
R. G. Mills ◽  
J. J. Bray
Keyword(s):  
Slow Release ◽  
Release Technique

MODEL FORMULATION FOR HYBRIDOMA CULTURES IN BATCH AND FED-BATCH MODE

2007 ◽  
Vol 40 (4) ◽  
pp. 181-186
Author(s):  
Penny Dorka ◽  
Christian Fischer ◽  
Hector M. Budman ◽  
Jeno M. Scharer
Keyword(s):  
Model Formulation ◽  
Fed Batch ◽  
Batch Mode

scholarly journals Description of a Decentralized Small Scale Digester for Treating Organic Wastes

Environments ◽  
2020 ◽  
Vol 7 (10) ◽  
pp. 78
Author(s):  
Rubén González ◽  
Daniel Blanco ◽  
Judith González-Arias ◽  
José García-Cascallana ◽  
Xiomar Gómez
Keyword(s):  
Organic Loading Rate ◽  
Small Scale ◽  
Complex Nature ◽  
Fed Batch ◽  
Batch Mode ◽  
Commercial Unit ◽  
Start Up ◽  
Volatile Solids ◽  
Vegetable Wastes ◽  
Feeding Substrate

This manuscript deals with the detailed design of a small digestion prototype intended as a commercial unit fully operational to cover the demand for decentralized treatment of wastes. These plants are highly affected by the complex nature of wastes giving rise to different operating problems that should be considered in detail. This paper describes the design and start-up strategy of a small-scale digestion plant with a volume of 8 m3 designed to operate with a hydrolysis pretreatment unit. The plant was designed to treat fruit and vegetable wastes as substrates derived from a local processing food factory. The performance of the plant during fed-batch operation was reported. The strategy of inoculating the reactor only to a third of its original volume and subsequently increasing the volume of the reactor by using the fed-batch mode was inadequate. The acid pH of the feeding substrate resulted in the application of a low organic loading rate with a volumetric variation of just 19.7 L/d. The performance of the plant was evaluated at non-steady state conditions and resulted in excessive destruction of volatile solids due to the low nitrogen content of the feeding substrate. The prototype reported a specific methane production of 232 L/kg volatile solids despite the low feeding rate supplemented.

Fed-Batch Cultivation in Baffled Shake Flasks

2011 ◽  
Vol 31 (14) ◽  
pp. 52-54 ◽  
Author(s):  
Johannes Hemmerich,
Keyword(s):  
Batch Cultivation ◽  
Fed Batch ◽  
Shake Flasks ◽  
Fed Batch Cultivation
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