Micro biochemical engineering to accelerate the design of industrial-scale downstream processes for biopharmaceutical proteins

The effect of oxygen (O2)-delignified pine kraft pulp pretreatment by high-purity, thermostable, and alkaline-tolerant xylanases on elemental chlorine free (ECF) bleaching of O2-delignification kraft pulp was studied. The study found that xylanase pretreatment preserved the intrinsic viscosity and yield of O2-delignified pulp while causing about 7% of delignification with high delignification selectivity. The xylanases with high purity, higher thermostability (75°C~80°C) in highly alkaline media (pH 8.0~9.5) could be applied on an industrial scale. Pulp pretreatment by the high-purity, thermostable, and alkaline tolerant xylanases could improve pulp brightness or reduce the chlorine dioxide (ClO2) consumption. In a D0ED1D2 bleaching sequence using the same amount of ClO2, the xylanase-pretreated pulp obtained a higher brightness (88.2% vs. 89.7% ISO) at the enzyme dose of 2 U/g pulp; or for the same brightness as control (88.2% ISO), the ClO2 dosage in the D0 stage was reduced by 27%, which represents a 16% savings in total ClO2 used for bleaching.

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Use of vent stack temperature as a feedforward variable for dissolver total titratable alkali (TTA) control

TAPPI Journal ◽

10.32964/tj17.05.261 ◽

2018 ◽

Vol 17 (05) ◽

pp. 261-269

Author(s):

Wei Ren ◽

Brennan Dubord ◽

Jason Johnson ◽

Bruce Allison

Keyword(s):

Feedback Control ◽

Current Practice ◽

Feedforward Control ◽

Control Variable ◽

Indirect Measurement ◽

Tight Control ◽

Transfer Lines ◽

Green Liquor ◽

Process Dynamics ◽

Downstream Processes

Tight control of raw green liquor total titratable alkali (TTA) may be considered an important first step towards improving the overall economic performance of the causticizing process. Dissolving tank control is made difficult by the fact that the unknown smelt flow is highly variable and subject to runoff. High TTA variability negatively impacts operational costs through increased scaling in the dissolver and transfer lines, increased deadload in the liquor cycle, under- and over-liming, increased energy consumption, and increased maintenance. Current practice is to use feedback control to regulate the TTA to a target value through manipulation of weak wash flow while simultaneously keeping dissolver density within acceptable limits. Unfortunately, the amount of variability reduction that can be achieved by feedback control alone is fundamentally limited by the process dynamics. One way to improve upon the situation would be to measure the smelt flow and use it as a feedforward control variable. Direct measurement of smelt flow is not yet possible. The use of an indirect measurement, the dissolver vent stack temperature, is investigated in this paper as a surrogate feedforward variable for dissolving tank TTA control. Mill trials indicate that significant variability reduction in the raw green liquor TTA is possible and that the control improvements carry through to the downstream processes.

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How to make Russia a prosperous industrial country (about the role of the oil production and refining complex)

Mezhdunarodnaja jekonomika (The World Economics) ◽

10.33920/vne-04-2007-03 ◽

2020 ◽

pp. 22-28

Author(s):

Yu. Kanataev

Keyword(s):

Precious Metals ◽

Oil Production ◽

Industrial Country ◽

Industrial Scale ◽

Deep Processing ◽

Domestic Technology ◽

Petrochemical Complex ◽

Russian Economy ◽

Further Development

The author, head of the Association of science-intensive enterprises having great experience in exploration of oil, precious metals, etc. in Russia and abroad (Austria, China, Israel, Ethiopia), first implemented on an industrial scale deep processing of oil using domestic technology, discusses the problems of further development of the Russian economy through the prism of their solutions to the main industries — oil and petrochemical complex.

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Protein Solvent Interaction: Transition of Protein-solvent Interaction Concept from Basic Research into Solvent Manipulation of Chromatography

Current Protein and Peptide Science ◽

10.2174/1389203718666171024121529 ◽

2018 ◽

Vol 20 (1) ◽

pp. 34-39 ◽

Cited By ~ 1

Author(s):

Tsutomu Arakawa ◽

Yoshiko Kita

Keyword(s):

Column Chromatography ◽

Rapid Development ◽

Academic Research ◽

Basic Research ◽

Protein Stabilization ◽

Special Issue ◽

Solvent Interaction ◽

Protein Formulations ◽

Solvent Additive ◽

Downstream Processes

Previously, we have reviewed in this journal (Arakawa, T., Kita, Y., Curr. Protein Pept. Sci., 15, 608-620, 2014) the interaction of arginine with proteins and various applications of this solvent additive in the area of protein formulations and downstream processes. In this special issue, we expand the concept of protein-solvent interaction into the analysis of the effects of solvent additives on various column chromatography, including mixed-mode chromatography. Earlier in our research, we have studied the interactions of such a variety of solvent additives as sugars, salts, amino acids, polymers and organic solvents with a variety of proteins, which resulted in mechanistic understanding on their protein stabilization and precipitation effects, the latter known as Hofmeister series. While such a study was then a pure academic research, rapid development of genetic engineering technologies and resultant biotechnologies made it a valuable knowledge in fully utilizing solvent additives in manipulation of protein solution, including column chromatography.

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