A strategy to determine operating parameters in tissue engineering hollow fiber bioreactors

The importance of the nanofiber webs increases rapidly due to their highly porous structure, narrow pore size, and distribution; specific surface area and compatibility with inorganics. Electrospinning has been introduced as one of the most efficient technique for the fabrication of polymeric nanofibers due to its ability to fabricate nanostructures with unique properties such as a high surface area and porosity. The process and the operating parameters affect the nanofiber fabrication and the application of nanofibers in various fields, such as sensors, tissue engineering, wound dressing, protective clothes, filtration, desalination, and distillation. In this review, a comprehensive study is presented on the parameters of electrospinning system including applications. More emphasis is given to the application of nanofibers in membrane distillation (MD). The research developments and the current situation of the nanofiber webs in MD are also discussed.

Download Full-text

Nutrient and metabolite gradients in mammalian cell hollow fiber bioreactors

The Canadian Journal of Chemical Engineering ◽

10.1002/cjce.5450690204 ◽

1991 ◽

Vol 69 (2) ◽

pp. 421-428 ◽

Cited By ~ 27

Author(s):

J. M. Piret ◽

D. A. Devens ◽

C. L. Cooney

Keyword(s):

Mammalian Cell ◽

Hollow Fiber ◽

Hollow Fiber Bioreactors

Download Full-text

A modeling study on the effects of membrane characteristics and operating parameters on physical absorption of CO2 by hollow fiber membrane contactor

Journal of Membrane Science ◽

10.1016/j.memsci.2011.06.029 ◽

2011 ◽

Vol 380 (1-2) ◽

pp. 21-33 ◽

Cited By ~ 58

Author(s):

Somnuk Boributh ◽

Suttichai Assabumrungrat ◽

Navadol Laosiripojana ◽

Ratana Jiraratananon

Keyword(s):

Hollow Fiber ◽

Hollow Fiber Membrane ◽

Operating Parameters ◽

Membrane Contactor ◽

Fiber Membrane ◽

Physical Absorption ◽

Modeling Study

Download Full-text

Poly(ε-Caprolactone) Hollow Fiber Membranes for the Biofabrication of a Vascularized Human Liver Tissue

Membranes ◽

10.3390/membranes10060112 ◽

2020 ◽

Vol 10 (6) ◽

pp. 112

Author(s):

Simona Salerno ◽

Franco Tasselli ◽

Enrico Drioli ◽

Loredana De Bartolo

Keyword(s):

Tissue Engineering ◽

Endothelial Cells ◽

Hollow Fiber ◽

Liver Tissue ◽

External Surface ◽

Internal Surface ◽

Hepatic Tissue ◽

Operational Parameters ◽

Human Organ ◽

Human Liver Tissue

The creation of a liver tissue that recapitulates the micro-architecture and functional complexity of a human organ is still one of the main challenges of liver tissue engineering. Here we report on the development of a 3D vascularized hepatic tissue based on biodegradable hollow fiber (HF) membranes of poly(ε-caprolactone) (PCL) that compartmentalize human hepatocytes on the external surface and between the fibers, and endothelial cells into the fiber lumen. To this purpose, PCL HF membranes were prepared by a dry-jet wet phase inversion spinning technique tailoring the operational parameters in order to obtain fibers with suitable properties. After characterization, the fibers were applied to generate a human vascularized hepatic unit by loading endothelial cells in their inner surface and hepatocytes on the external surface. The unit was connected to a perfusion system, and the morpho-functional behavior was evaluated. The results demonstrated the large integration of endothelial cells with the internal surface of individual PCL fibers forming vascular-like structures, and hepatocytes covered completely the external surface and the space between fibers. The perfused 3D hepatic unit retained its functional activity at high levels up to 18 days. This bottom-up tissue engineering approach represents a rational strategy to create relatively 3D vascularized tissues and organs.

Download Full-text