Effect of ferric tartrate/sodium hydroxide solvent pretreatment on enzyme hydrolysis of cellulose in corn residue

Selulosa adalah polisakarida rantai panjang penyusun serat pada tumbuhan. Hidrolisis selulosa dengan alkali kuat menghasilkan asam oksalat, asam asetat dan asam formiat. Limbah serbuk kayu jati berpotensi untuk dijadikan bahan baku pembuatan asam oksalat karena kandungan selulosa yang cukup tinggi. Hidrolisis yang dilakukan pada penelitian ini menggunakan natrium hidroksida (NaOH) sebagai zat penghidrolisis. Purifikasi asam oksalat dilakukan dengan penambahan kalsium klorida dan asam sulfat. Penelitian ini mempelajari pengaruh konsentrasi natrium hidroksida dan waktu reaksi terhadap yield asam oksalat. Produk tertinggi dengan yield 20% dicapai pada penggunaan serbuk kayu jati kasar dengan waktu hidrolisis 60 menit dan konsentrasi NaOH 1 N.Cellulose is a long chain fiber polysaccharide contained in plants. Hydrolysis of cellulose with strong alkali produces oxalic acid, acetic acid and formic acid. Waste from teak wood in powder formhas the potential to be used as raw material for the manufacture of oxalic acid because the content of cellulose is high enough. Sodium hydroxide (NaOH) as a hydrolysis agent was used in this study. Purification of formed oxalic acid was carried out by addition of calcium chloride and sulfuric acid. Our research studied the effect of sodium hydroxide concentration and reaction time on oxalic acid yield. The highest product with a yield of 20% was achieved on the use of coarse powder of teak wood waste with a hydrolysis time of 60 minutes and the concentration of NaOH 1 N.

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Combined enzyme hydrolysis of cellulose and yeast fermentation

Biotechnology and Bioengineering ◽

10.1002/bit.260200814 ◽

1978 ◽

Vol 20 (8) ◽

pp. 1291-1293 ◽

Cited By ~ 29

Author(s):

John J. Savarese ◽

Steven D. Young

Keyword(s):

Enzyme Hydrolysis ◽

Yeast Fermentation ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

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ENZYME HYDROLYSIS OF PURIFIED HCG

Acta Endocrinologica ◽

10.1530/acta.0.061s219 ◽

1969 ◽

Vol 61 (1_Suppl) ◽

pp. S219

Author(s):

Carl Beling ◽

Ronald Stark

Keyword(s):

Enzyme Hydrolysis ◽

Hydrolysis Of

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Deinking of recycled paper by coupling of the enzyme endo-β-1,4-D-glucanasa and the cellulose, and by using a laboratory flotation column

MRS Advances ◽

10.1557/adv.2020.390 ◽

2020 ◽

Vol 5 (52-53) ◽

pp. 2669-2678

Author(s):

Jeovani González P. ◽

Ramiro Escudero G

Keyword(s):

Amino Acids ◽

Sodium Hydroxide ◽

Paper Industry ◽

Computational Simulation ◽

Cellulose Fibers ◽

Residual Water ◽

Chemical Reagent ◽

Recycled Paper ◽

Flotation Column ◽

Hydrolysis Of

AbstractDeinking of recycled office (MOW) paper was carried out by using a flotation column and adding separately sodium hydroxide, and the enzyme Cellulase Thricodema Sp., as defibrillators.The de-inked cellulose fibers were characterized according to the standards of the paper industry, to compare the efficiency of the deinking of each chemical reagent used to hydrolyze the fibers and defibrillate them.The computational simulation of the molecular coupling between the enzyme and cellulose was performed, to establish the enzyme-cellulose molecular complex and then to identify the principal amino-acids of endo-β-1,4-D-glucanase in this molecular link, which are responsible for the hydrolysis of the cellulose.Experimental results show the feasibility to replace sodium hydroxide with the enzyme Cellulase Thricodema Sp., by obtaining deinked cellulose with similar optical and physical properties.The use of the enzyme instead of sodium hydroxide avoids the contamination of the residual water; in addition to that, the column is operated more easily, taking into consideration that the pH of the system goes from alkaline to neutral.

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Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

Current Nanoscience ◽

10.2174/1573413714666180611081759 ◽

2019 ◽

Vol 15 (3) ◽

pp. 296-303 ◽

Cited By ~ 5

Author(s):

Swapnil Gaikwad ◽

Avinash P. Ingle ◽

Silvio Silverio da Silva ◽

Mahendra Rai

Keyword(s):

Catalytic Activity ◽

Enzymatic Hydrolysis ◽

Immobilized Enzyme ◽

Free Enzyme ◽

Magnetic Nanomaterials ◽

Sugar Production ◽

Cellulase Enzyme ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

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