Non-Newtonian rheology in suspension cell cultures significantly impacts bioreactor shear stress quantification

2018 ◽  
Vol 115 (8) ◽  
pp. 2101-2113 ◽  
Author(s):  
Alex Wyma ◽  
Leonardo Martin-Alarcon ◽  
Tylor Walsh ◽  
Tannin A. Schmidt ◽  
Ian D. Gates ◽  
...  
Keyword(s):  
Shear Stress ◽  
Cell Cultures ◽  
Suspension Cell ◽  
Suspension Cell Cultures

Salicylic acid differentially affects suspension cell cultures of Lotus japonicus and one of its non-symbiotic mutants

2009 ◽  
Vol 72 (4-5) ◽  
pp. 469-483 ◽  
Author(s):  
Fiorenza Bastianelli ◽  
Alex Costa ◽  
Marco Vescovi ◽  
Enrica D’Apuzzo ◽  
Michela Zottini ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Cell Cultures ◽  
Lotus Japonicus ◽  
Suspension Cell ◽  
Suspension Cell Cultures

Gymnemic Acid Production in Suspension Cell Cultures of Gymnema sylvestre

2006 ◽  
Vol 6 (10) ◽  
pp. 2263-2268 ◽  
Author(s):  
C. Subathra Devi ◽  
S. Murugesh . ◽  
V. Mohana Srinivasan .
Keyword(s):  
Cell Cultures ◽  
Acid Production ◽  
Suspension Cell ◽  
Gymnema Sylvestre ◽  
Gymnemic Acid ◽  
Suspension Cell Cultures

scholarly journals Phosphate and phosphite have a differential impact on the proteome and phosphoproteome of Arabidopsis suspension cell cultures

2020 ◽  
Author(s):  
Devang Mehta ◽  
Mina Ghahremani ◽  
Maria Pérez‐Fernández ◽  
Maryalle Tan ◽  
Pascal Schläpfer ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Suspension Cell ◽  
Differential Impact ◽  
Suspension Cell Cultures

scholarly journals Putrescine Promotes Betulin Accumulation in Suspension Cell Cultures of Betula platyphylla by Regulating NO and NH4+ Production

Forests ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1336
Author(s):  
Guizhi Fan ◽  
Tingting Zhang ◽  
Yingtian Liu ◽  
Yaguang Zhan ◽  
Baojiang Zheng
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Cell Cultures ◽  
Regulatory Mechanism ◽  
Glutamate Synthase ◽  
Suspension Cell ◽  
Suspension Cells ◽  
Synthetic Enzymes ◽  
Nos Inhibitor ◽  
Suspension Cell Cultures

Putrescine (Put) can enhance secondary metabolite production, but its intrinsic regulatory mechanism remains unclear. In this study, Put treatment promoted betulin production and gene expression of lupeol synthase (LUS), one of betulin synthetic enzymes. The maximum betulin content and gene expression level of LUS was 4.25 mg·g−1 DW and 8.25 at 12 h after 1 mmol·L−1 Put treatment, approximately two- and four-times that in the control, respectively. Put treatment increased the content of nitric oxide (NO) and its biosynthetic enzyme activity of nitrate reductase (NR) and NO synthase (NOS). Pretreatment of the birch suspension cells with NO-specific scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline- 1-oxyl-3-oxide (cPTIO), NR inhibitor sodium azide (NaN3), and NOS inhibitor NG-nitro-L-Arg methyl ester (L-NAME) decreased Put-triggered NO generation and blocked Put-induced betulin production. Put treatment improved the content of NH4+ and its assimilation enzyme activity of glutamate synthase and glutamate dehydrogenase. NH4+ supplementation also promoted NO and betulin production. Thus, the above data indicated that Put-induced NO was essential for betulin production. NO derived from NR, NOS, and NH4+ mediated betulin production in birch suspension cell cultures under Put treatment.

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