Integrating high cell density cultures with adapted laboratory evolution for improved Gag‐HA virus‐like particles production in stable insect cell lines

2021 ◽  
Author(s):  
Bárbara Fernandes ◽  
Ricardo Correia ◽  
Marcos Sousa ◽  
Manuel JT Carrondo ◽  
Paula M Alves ◽  
...  
Keyword(s):  
Cell Density ◽  
Cell Lines ◽  
Insect Cell ◽  
High Cell Density ◽  
High Cell ◽  
Laboratory Evolution ◽  
Virus Like Particles ◽  
Insect Cell Lines ◽  
High Cell Density Cultures

scholarly journals Hormonal regulation of the transformation phenotype in simian virus 40-transformed rat embryonic preadipose cell lines.

1984 ◽  
Vol 4 (4) ◽  
pp. 712-721 ◽  
Author(s):  
S Yasumoto
Keyword(s):  
Cell Density ◽  
Cell Lines ◽  
Hormonal Regulation ◽  
Growth Regulation ◽  
High Cell Density ◽  
Calf Serum ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
High Cell

The regulation of transformed phenotypes was studied in newly isolated preadipose cell lines which were established after infection with simian virus 40 tsA58 dl2009. The clonal cell lines isolated exhibited most of the characteristics typical of transformed cells. The transformants, however, were able to differentiate into adipocytes in the presence of low calf serum (0.5%) and a combination of several hormones, including hydrocortisone and insulin. Treatment with insulin alone stimulated the growth of these cells but did not induce lipid accumulation without added hydrocortisone. The effect of hydrocortisone was accompanied by a restoration of growth control in the transformants after they reached high cell density. The blot hybridization analysis of cellular DNAs digested by restriction enzymes revealed that simian virus 40 genomes were integrated at multiple separate sites at which a head-to-tail oligomeric insertion took place. Large T antigen was synthesized in growing cells but was regulated at high cell density when cells were committed to differentiate by glucocorticoids. These results suggest that the glucocorticoid hydrocortisone is capable of restoring growth regulation at high cell densities to simian virus 40-transformed preadipose cell lines.

Design and validation of a pulsatile perfusion bioreactor for 3D high cell density cultures

2009 ◽  
Vol 104 (6) ◽  
pp. 1215-1223 ◽  
Author(s):  
Julie A. Chouinard ◽  
Serge Gagnon ◽  
Marc G. Couture ◽  
Alain Lévesque ◽  
Patrick Vermette
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Perfusion Bioreactor ◽  
High Cell ◽  
Pulsatile Perfusion ◽  
High Cell Density Cultures

scholarly journals Hybrid observer for high cell density cultures exhibiting overflow metabolism

2020 ◽  
Author(s):  
Lisbel Barzaga Martell ◽  
Manuel Duarte-Mermoud ◽  
Francisco Ibañez Espinel ◽  
Bastian Gamboa Labbé ◽  
José Pérez-Correa
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Overflow Metabolism ◽  
High Cell ◽  
High Cell Density Cultures

Adaptive laboratory evolution of stable insect cell lines for improved HIV-Gag VLPs production

2020 ◽  
Vol 307 ◽  
pp. 139-147 ◽  
Author(s):  
Bárbara Fernandes ◽  
João Vidigal ◽  
Ricardo Correia ◽  
Manuel J.T. Carrondo ◽  
Paula M. Alves ◽  
...  
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
Insect Cell Lines
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