Investigations of high cell density baculovirus infection using Sf9 and High Five insect cell lines in the low-cost SF-1 medium

1995 ◽  
pp. 313-315 ◽  
Author(s):  
E.-J. Schlaeger ◽  
J. Stricker ◽  
J. Wippler ◽  
M. Foggetta
Keyword(s):  
Cell Density ◽  
Cell Lines ◽  
Insect Cell ◽  
High Cell Density ◽  
Low Cost ◽  
High Cell ◽  
Baculovirus Infection ◽  
Insect Cell Lines

scholarly journals Hormonal regulation of the transformation phenotype in simian virus 40-transformed rat embryonic preadipose cell lines.

1984 ◽  
Vol 4 (4) ◽  
pp. 712-721 ◽  
Author(s):  
S Yasumoto
Keyword(s):  
Cell Density ◽  
Cell Lines ◽  
Hormonal Regulation ◽  
Growth Regulation ◽  
High Cell Density ◽  
Calf Serum ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
High Cell

The regulation of transformed phenotypes was studied in newly isolated preadipose cell lines which were established after infection with simian virus 40 tsA58 dl2009. The clonal cell lines isolated exhibited most of the characteristics typical of transformed cells. The transformants, however, were able to differentiate into adipocytes in the presence of low calf serum (0.5%) and a combination of several hormones, including hydrocortisone and insulin. Treatment with insulin alone stimulated the growth of these cells but did not induce lipid accumulation without added hydrocortisone. The effect of hydrocortisone was accompanied by a restoration of growth control in the transformants after they reached high cell density. The blot hybridization analysis of cellular DNAs digested by restriction enzymes revealed that simian virus 40 genomes were integrated at multiple separate sites at which a head-to-tail oligomeric insertion took place. Large T antigen was synthesized in growing cells but was regulated at high cell density when cells were committed to differentiate by glucocorticoids. These results suggest that the glucocorticoid hydrocortisone is capable of restoring growth regulation at high cell densities to simian virus 40-transformed preadipose cell lines.

scholarly journals Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture

2004 ◽  
Vol 44 (3) ◽  
pp. 93-102 ◽  
Author(s):  
Volker M. Gorenflo ◽  
Tom A. Pfeifer ◽  
Gary Lesnicki ◽  
Emily M. Kwan ◽  
Thomas A. Grigliatti ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Cell Line ◽  
Cell Density ◽  
Insect Cell ◽  
High Cell Density ◽  
Insect Cell Line ◽  
Perfusion Culture ◽  
Factor X ◽  
High Cell

HIGH CELL DENSITY IN INSECT CELL CULTURES

1994 ◽  
pp. 494-503 ◽  
Author(s):  
S. Reuveny ◽  
C.W. Kemp ◽  
J. Shiloach
Keyword(s):  
Cell Density ◽  
Cell Cultures ◽  
Insect Cell ◽  
High Cell Density ◽  
High Cell ◽  
Insect Cell Cultures

Hormonal regulation of the transformation phenotype in simian virus 40-transformed rat embryonic preadipose cell lines

1984 ◽  
Vol 4 (4) ◽  
pp. 712-721
Author(s):  
S Yasumoto
Keyword(s):  
Cell Density ◽  
Cell Lines ◽  
Hormonal Regulation ◽  
Growth Regulation ◽  
High Cell Density ◽  
Calf Serum ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
High Cell

The regulation of transformed phenotypes was studied in newly isolated preadipose cell lines which were established after infection with simian virus 40 tsA58 dl2009. The clonal cell lines isolated exhibited most of the characteristics typical of transformed cells. The transformants, however, were able to differentiate into adipocytes in the presence of low calf serum (0.5%) and a combination of several hormones, including hydrocortisone and insulin. Treatment with insulin alone stimulated the growth of these cells but did not induce lipid accumulation without added hydrocortisone. The effect of hydrocortisone was accompanied by a restoration of growth control in the transformants after they reached high cell density. The blot hybridization analysis of cellular DNAs digested by restriction enzymes revealed that simian virus 40 genomes were integrated at multiple separate sites at which a head-to-tail oligomeric insertion took place. Large T antigen was synthesized in growing cells but was regulated at high cell density when cells were committed to differentiate by glucocorticoids. These results suggest that the glucocorticoid hydrocortisone is capable of restoring growth regulation at high cell densities to simian virus 40-transformed preadipose cell lines.

Baculovirus infection influences host protein expression in two established insect cell lines

2010 ◽  
Vol 56 (9) ◽  
pp. 1237-1245 ◽  
Author(s):  
Holly J.R. Popham ◽  
James J. Grasela ◽  
Cynthia L. Goodman ◽  
Arthur H. McIntosh
Keyword(s):  
Protein Expression ◽  
Cell Lines ◽  
Insect Cell ◽  
Host Protein ◽  
Baculovirus Infection ◽  
Insect Cell Lines
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