scholarly journals Preparation of thermosensitive PNIPAm‐based copolymer coated cytodex 3 microcarriers for efficient non‐enzymatic cell harvesting during 3D culturing.

2021 ◽  
Author(s):  
Haile Fentahun Darge ◽  
Shun‐Hao Chuang ◽  
Juin‐Yih Lai ◽  
Shuian‐Yin Lin ◽  
Hsieh‐Chih Tsai
Keyword(s):  
Cell Harvesting ◽  
Cytodex 3

scholarly journals Preparation of thermosensitive PNIPAm-based copolymer coated cytodex 3 microcarriers for efficient non-enzymatic cell harvesting during 3D culturing

2021 ◽  
Author(s):  
Haile Darge ◽  
Shun-Hao Chuang ◽  
Juin-Yih Lai ◽  
Shuian-Yin Lin ◽  
Hsieh-Chih Tsai
Keyword(s):  
Cell Attachment ◽  
Polymer Surface ◽  
Contact Angles ◽  
Cell Detachment ◽  
Thermosensitive Polymer ◽  
Sem Image ◽  
Cell Harvesting ◽  
Cytodex 3 ◽  
Maximum Cell ◽  
Number Of Cells

Enzymatic detachment of cells might damage important features of cells and could affect subsequent function of cells in various applications. Therefore, non-enzymatic cell detachment using thermosensitive polymer matrix is necessary for maintaining cell quality after harvesting. In this study, we synthesized thermosensitive PNIPAm-co-AAc-b-PS and PNIPAm-co-AAm-b-PS copolymers and LCST was tuned near to body temperature. Then, polymer solutions (5% w/v, 10% w/v, and 20% w/v) were spin coated to prepare films for cell adhesion and thermal-induced cell detachment. The apha-step analysis and SEM image of the films suggested that the thickness of the films depends on the molecular weight and concentration which ranged from 206 nm to 1330 nm for PNIPAm-co-AAc-b-PS and 97.5 nm to 497 nm for PNIPAm-co-AAm-b-PS. The contact angles of the films verified that the polymer surface was moderately hydrophilic at 37°C. From cell attachment and detachment studies, RAW264.7 cells, were convincingly proliferated on the films to a confluent of >80 % within 48 days. However, relatively more cells were grown on PNIPAm-co-AAm-b-PS (5%w/v) films and thermal-induced cell detachment was more abundant in this formulation. As a result, commercial cytodex 3 microcarrier was coated with PNIPAm-co-AAm-b-PS (5%w/v) and interestingly enhanced cell detachment with preserved potential of recovery was observed at low temperature during 3D culturing. Thus, surface modification of microcarriers with PNIPAm-co-AAm-b-PS could be vital strategy for non-enzymatic cell dissociation and able to achieve adequate number of cells with maximum cell viability, and functionality for various cell-based applications. Keywords: surface coated microcarriers; thermosensitive polymer; non-enzymatic cell detachment

scholarly journals Engineering Cyanobacterial Cell Morphology for Enhanced Recovery and Processing of Biomass

2017 ◽  
Vol 83 (9) ◽  
Author(s):  
Adam Jordan ◽  
Jenna Chandler ◽  
Joshua S. MacCready ◽  
Jingcheng Huang ◽  
Katherine W. Osteryoung ◽  
...  
Keyword(s):  
Cell Division ◽  
Cell Morphology ◽  
Dynamic Range ◽  
Enhanced Recovery ◽  
Downstream Processing ◽  
Crop Species ◽  
Content Type ◽  
Alternative Crop ◽  
Cell Harvesting ◽  
Broad Dynamic Range

ABSTRACT Cyanobacteria are emerging as alternative crop species for the production of fuels, chemicals, and biomass. Yet, the success of these microbes depends on the development of cost-effective technologies that permit scaled cultivation and cell harvesting. Here, we investigate the feasibility of engineering cell morphology to improve biomass recovery and decrease energetic costs associated with lysing cyanobacterial cells. Specifically, we modify the levels of Min system proteins in Synechococcus elongatus PCC 7942. The Min system has established functions in controlling cell division by regulating the assembly of FtsZ, a tubulin-like protein required for defining the bacterial division plane. We show that altering the expression of two FtsZ-regulatory proteins, MinC and Cdv3, enables control over cell morphology by disrupting FtsZ localization and cell division without preventing continued cell growth. By varying the expression of these proteins, we can tune the lengths of cyanobacterial cells across a broad dynamic range, anywhere from an ∼20% increased length (relative to the wild type) to near-millimeter lengths. Highly elongated cells exhibit increased rates of sedimentation under low centrifugal forces or by gravity-assisted settling. Furthermore, hyperelongated cells are also more susceptible to lysis through the application of mild physical stress. Collectively, these results demonstrate a novel approach toward decreasing harvesting and processing costs associated with mass cyanobacterial cultivation by altering morphology at the cellular level. IMPORTANCE We show that the cell length of a model cyanobacterial species can be programmed by rationally manipulating the expression of protein factors that suppress cell division. In some instances, we can increase the size of these cells to near-millimeter lengths with this approach. The resulting elongated cells have favorable properties with regard to cell harvesting and lysis. Furthermore, cells treated in this manner continue to grow rapidly at time scales similar to those of uninduced controls. To our knowledge, this is the first reported example of engineering the cell morphology of cyanobacteria or algae to make them more compatible with downstream processing steps that present economic barriers to their use as alternative crop species. Therefore, our results are a promising proof-of-principle for the use of morphology engineering to increase the cost-effectiveness of the mass cultivation of cyanobacteria for various sustainability initiatives.

scholarly journals Autoimmune HIT due to apheresis catheter heparin flushes for stem cell harvesting before autotransplantation for myeloma

Blood ◽  
2017 ◽  
Vol 130 (14) ◽  
pp. 1679-1682 ◽  
Author(s):  
Hira Mian ◽  
Theodore E. Warkentin ◽  
Jo-Ann I. Sheppard ◽  
Anna MacDonald ◽  
Lori-Ann Linkins ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Harvesting

Correlation Coefficients Between Several Parameters and CD34+Cell Yield in Peripheral Blood Stem Cell Harvesting by Apheresis

2001 ◽  
Vol 10 (1) ◽  
pp. 17-18
Author(s):  
José R. Borbolla ◽  
Alejandro Nájera ◽  
Manuel A. López-Hernández ◽  
Mauricio González ◽  
Alberto Siller ◽  
...  
Keyword(s):  
Stem Cell ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cell ◽  
Correlation Coefficients ◽  
Blood Stem Cell ◽  
Cell Yield ◽  
Cell Harvesting

scholarly journals A minimal standardized human bone marrow microphysiological system to assess resident cell behavior during normal and pathological processes

2022 ◽  
Author(s):  
Thibault Voeltzel ◽  
Gaëlle Fossard ◽  
Michaël Degaud ◽  
Kevin Geistlich ◽  
Nicolas Gadot ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Viable Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Behavior ◽  
Cell Harvesting ◽  
Resident Cell ◽  
Level Of Analysis

We provide an easy to access microphysiological standardized system approaching the human bone marrow complexity to a first level of analysis by in situ imaging or by viable cell harvesting of processes taking place within this ecosystem.

EVOLUTION OF THE CAPACITANCE OF CYTODEX 3 MICROBEADS SUSPENDED IN GROWTH MEDIUM

1994 ◽  
pp. 256-258 ◽  
Author(s):  
V. Degouys ◽  
F.D. Menozzi ◽  
D.A. Dubois ◽  
L. Fabry ◽  
A.O.A. Miller
Keyword(s):  
Growth Medium ◽  
Cytodex 3

Normal T, B, and NK cell counts in healthy donors at 1 year after blood stem cell harvesting

Blood ◽  
2000 ◽  
Vol 95 (9) ◽  
pp. 2993-2994 ◽  
Author(s):  
Jan Storek ◽  
Monja A. Dawson ◽  
David G. Maloney
Keyword(s):  
Stem Cell ◽  
Nk Cell ◽  
Blood Stem Cell ◽  
Cell Counts ◽  
Cell Harvesting ◽  
Healthy Donors

Gr/TiO2 Films with Light-Controlled Positive/Negative Charge for Cell Harvesting Application

2020 ◽  
Vol 6 (4) ◽  
pp. 2020-2028 ◽  
Author(s):  
Xiaojun Long ◽  
Yang Yi ◽  
Xiaozhao Wang ◽  
Xiyue Duan ◽  
Yuan Sun ◽  
...  
Keyword(s):  
Negative Charge ◽  
Tio2 Films ◽  
Cell Harvesting

scholarly journals Cost-Effectiveness of the Use of Autologous Cell Harvesting Device Compared to Standard of Care for Treatment of Severe Burns in the United States

2019 ◽  
Vol 36 (7) ◽  
pp. 1715-1729 ◽  
Author(s):  
Stacey Kowal ◽  
Eliza Kruger ◽  
Pinar Bilir ◽  
James H. Holmes ◽  
William Hickerson ◽  
...  
Keyword(s):  
United States ◽  
Cost Effectiveness ◽  
Standard Of Care ◽  
The United States ◽  
Severe Burns ◽  
Autologous Cell ◽  
Cell Harvesting
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