LR12-peptide quantitation in whole blood by RP-HPLC and intrinsic fluorescence detection: Validation and pharmacokinetic study

Abstract Modified AOAC 991.31 and AOAC 2000.03 methods for the simultaneous determination of total aflatoxins (AFs), aflatoxin B1, and ochratoxin A (OTA) in processed cereal-based foods by RP-HPLC coupled with fluorescence detection were validated. A KOBRA® Cell derivatization system was used to analyze total AFs. One of the modifications was the extraction procedure of mycotoxins. Both AFs and OTA were extracted with methanol–water (75 + 25, v/v) and purified with an immunoaffinity column before HPLC analysis. The modified methods were validated by measuring the specificity, selectivity, linearity, sensitivity, accuracy, repeatability, reproducibility, recovery, LOD, and LOQ parameters. The validated methods were successfully applied for the simultaneous determination of mycotoxins in 81 processed cereal-based foods purchased in Turkey. These rapid, sensitive, simple, and validated methods are suitable for the simultaneous determination of AFs and OTA in the processed cereal-based foods.

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Development of validated RP HPLC method with fluorescence detection for simultaneous quantification of sacubitril and valsartan from rat plasma

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1080/10826076.2018.1436070 ◽

2018 ◽

Vol 41 (5) ◽

pp. 246-252 ◽

Cited By ~ 4

Author(s):

Mahesh Attimarad ◽

Sree Harsha Nagaraja ◽

Anroop Balachandran Nair ◽

Bandar Essa Aldhubaib ◽

Venugopala Narayanaswamy Katharigatta

Keyword(s):

Fluorescence Detection ◽

Rat Plasma ◽

Hplc Method ◽

Simultaneous Quantification ◽

Rp Hplc

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Determination of Tofacitinib in Mice Whole Blood on Dried Blood Spots Using LC–ESI–MS/MS: Application to Pharmacokinetic Study in Mice

Drug Research ◽

10.1055/a-0677-3066 ◽

2018 ◽

Vol 69 (06) ◽

pp. 330-336 ◽

Cited By ~ 1

Author(s):

Abhishek Dixit ◽

Sadanand Rangnathrao Mallurwar ◽

Suresh P Sulochana ◽

Mohd Zainuddin ◽

Ramesh Mullangi

Keyword(s):

Pharmacokinetic Study ◽

Whole Blood ◽

Internal Standard ◽

Dried Blood Spots ◽

Assay Method ◽

Blood Spots ◽

Novel Method ◽

Positive Ion ◽

Regulatory Guideline

AbstractA simple, sensitive and rapid assay method has been developed and validated as per regulatory guideline for the estimation of tofacitinib on mice dried blood spots (DBS) using liquid chromatography coupled to tandem mass spectrometry with electro spray ionization in the positive-ion mode. The method employs liquid extraction of tofacitinib from DBS disk of mice whole blood followed by chromatographic separation using 5 mM ammonium acetate (pH 6.5):acetonitrile (20:80, v/v) at a flow rate of 0.60 mL/min on an X-Terra Phenyl column with a total run time 2.5 min. The MS/MS ion transitions monitored were m/z 313→149 for tofacitinib and m/z 316→149 for the internal standard (13C3, 15N-tofacitinib). The assay was linear in the range of 0.99–1980 ng/mL. The intra- and inter-day precision was in the range of 1.17–10.3 and 3.37–10.9%, respectively. Stability studies showed that tofacitinib was stable on DBS cards for one month. This novel method has been applied to analyze the DBS samples of tofacitinib obtained from a pharmacokinetic study in mice.

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